Method for rapidly detecting benzopyrene in soil

A detection method, the technology of benzopyrene, applied in the field of environmental monitoring, can solve problems such as waste of time and mobile phase, threat to the safety of experimenters, complicated pretreatment methods, etc., to achieve simple operation, reduce inhalation or skin contact with benzopyrene The probability of detection and the effect of low detection cost

Inactive Publication Date: 2016-04-20
SUZHOU GUOHUAN ENVIRONMENT DETECTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to different detection objects, separation, extraction, and enrichment methods are also different. At present, my country has already had national standard determination methods in air, water and food, but there is no detection method for benzopyrene in soil. Establish a Accurate, fast and simple method for detecting benzopyrene in soil is of great significance to the ecological environment and human health
[0004] In addition, in the current benzopyrene detection method, the sample pretreatment method is complex and the steps are cumbersome, and it is easy to inhale or skin contact with benzopyrene due to careless operation during the pretreatment sample process, which brings safety hazards to the experimenters. great threat
In addition, the current benzopyrene detection method generally takes a long time to detect, wastes time and mobile phase, and the detection cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Instrument: Agilent high performance liquid chromatography, type 1200; chromatographic column is ODS column (5 μm, 4.6mm*150mm); mobile phase: acetonitrile-water; injection volume: 10 μL; detector is fluorescence detector, excitation wavelength 290nm, The emission wavelength is 430nm.

[0021] Reagents: The reagents used in the experiment are of analytical grade or superior grade, and the water used is Millipore ultrapure water.

[0022] Benzopyrene standard substance: purchased from the Research Center of Traditional Chinese Medicine Standard Reference Substance.

[0023] Soil reference materials: GSBZ50012-88 (ESS-2) was purchased from China National Environmental Monitoring Station and GBW07453 (GSS-24) was purchased from National Research Center for Reference Materials (Beijing).

[0024] 1# sample and 2# sample: collected from the soil near the refinery and the soil polluted by coal tar and asphalt respectively.

[0025] A kind of rapid detection method of benzop...

Embodiment 2

[0038] Instrument: Agilent high performance liquid chromatography, type 1200; chromatographic column is ODS column (5 μm, 4.6mm*150mm); mobile phase: acetonitrile-water; injection volume: 10 μL; detector is fluorescence detector, excitation wavelength 290nm, The emission wavelength is 430nm.

[0039] Reagents: The reagents used in the experiment are of analytical grade or superior grade, and the water used is Millipore ultrapure water.

[0040] Benzopyrene standard substance: purchased from the Research Center of Traditional Chinese Medicine Standard Reference Substance.

[0041] Soil reference materials: GSBZ50012-88 (ESS-2) was purchased from China National Environmental Monitoring Station and GBW07453 (GSS-24) was purchased from National Research Center for Reference Materials (Beijing).

[0042] 1# sample and 2# sample: collected from the soil near the refinery and the soil polluted by coal tar and asphalt respectively.

[0043] A kind of rapid detection method of benzop...

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Abstract

The invention discloses a method for rapidly detecting benzopyrene in soil. The method is characterized by comprising the following steps of S01, preparation of a test article solution; S02, preparation of a standard article solution; S03, detection: sequentially injecting the standard article solution and the test article solution of the benzopyrene into HPLC (high performance liquid chromatography), wherein the detection conditions of the HPLC include an ODS (octadecylsilyl) column; column temperature: 30 DEG C; a moving phase: trapezoidal elution: 0-3min, and 0-20% of acetonitrile; 3-10min, and 20-25% of acetonitrile; 10-13min, and 25-60% of acetonitrile; 13-20min, and 60-100% of acetonitrile; a fluorescent detector: laser wavelength of 390nm, and transmitting wavelength of 430nm. The method for rapidly detecting the benzopyrene in the soil has the advantages that the elution time is short, the complete elution of the benzopyrene is guaranteed, the time and the moving phase are saved, and the detection cost is low.

Description

technical field [0001] The invention relates to a rapid detection method for benzopyrene in soil, belonging to the technical field of environmental monitoring methods. Background technique [0002] Benzopyrene, also known as benzo(α)pyrene, is a common highly active indirect carcinogen. After it enters the body through inhalation or diet, it can easily combine with human DNA and disrupt the protein synthesis program of the human body. It plays the role of a "trigger" in the activity of DNA: as long as a very small amount of benzopyrene at nanogram level can change the structure, direction and function of DNA; DNA combined with benzopyrene, synthesized cells No longer normal cells, but tumors, which lead to cancer. It is easy to remain in water, soil and crops. Many countries have conducted investigations on the content of benzopyrene in soil. The residual concentration depends on the nature and distance of the source of pollution. Benzopyrene is found in the soil on both ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/08G01N30/14
CPCG01N30/02G01N30/08G01N30/14G01N2030/027G01N2030/062
Inventor 崔晓晓熊维维李慧
Owner SUZHOU GUOHUAN ENVIRONMENT DETECTION
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