Method for efficiently and accurately measuring glycidyl esters in grease

A technology for accurate determination of glycidyl esters, used in measuring devices, instruments, scientific instruments, etc., can solve the problems of long detection time and efficient detection of unfavorable samples, and achieve the effect of shortening detection time, saving elution time, and efficient detection.

Inactive Publication Date: 2019-12-03
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current direct method takes too long to detect, which is not conducive to the efficient detection of samples.

Method used

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  • Method for efficiently and accurately measuring glycidyl esters in grease
  • Method for efficiently and accurately measuring glycidyl esters in grease
  • Method for efficiently and accurately measuring glycidyl esters in grease

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0064] This example provides an efficient and accurate method for the determination of GEs in oils. The determination of the content of glycidyl palmitate (C16:0-GE) in palm oil includes the following steps:

[0065] (1) Prepare the standard solution: Dilute the glycidyl palmitate standard with methanol to obtain a standard solution with a C16:0-GE concentration of 1000 ng / mL, and further dilute with methanol to obtain 5, 10, 50, 100, 200, 400 ng / mL mL standard solution, ready for use;

[0066] (2) Pretreatment of the sample to be tested: Accurately weigh 0.500g of palm oil sample, dissolve it with methyl tert-butyl ether / ethyl acetate (3:1, V / V), so that the concentration of the sample to be tested is 240mg / mL , C18 solid-phase extraction cartridge (500 mg) was activated with 2 mL of methanol for 3 times, pipetted 100 μL of the sample solution to be tested onto the C18 solid-phase extraction cartridge, eluted the sample with 2 mL of methanol for 3 times, and added 2 mL of n-h...

Embodiment 2

[0082] This example provides an efficient and accurate method for the determination of GEs in oils and fats to determine the content of glycidyl stearate (C18:0-GE) in rice bran oil:

[0083] (1) Prepare the standard solution: Dilute the standard glycidyl stearate with methanol to obtain a standard solution with a C18:0-GE concentration of 1000ng / mL, and further dilute with methanol to obtain 5, 10, 50, 100, 200, 400ng / mL standard solution, ready for use;

[0084] (2) Pretreatment of the sample to be tested: Accurately weigh 1.000g of rice bran oil sample, dissolve it with methyl tert-butyl ether / ethyl acetate (4:1, V / V), so that the concentration of the sample to be tested is 250 mg / mL, C18 solid-phase extraction cartridge (500mg) was activated with 3 mL of methanol for 3 times, pipetted 100 μL of sample solution onto the C18 solid-phase extraction cartridge, eluted the sample with 3 mL of methanol for 3 times, and added 2 mL of n-hexane / ethyl acetate after blowing with nit...

Embodiment 3

[0100] This example provides an efficient and accurate method for the determination of GEs in fats and oils to determine the content of glycidyl oleate (C18:1-GE) in rapeseed oil, including the following steps:

[0101] (1) Configuration of the standard solution: dilute the glycidyl oleate standard product with methanol to obtain a standard solution with a concentration of 1000ng / mL of C18:1-GE, and further dilute with methanol to obtain 5, 10, 50, 100, 200, 400ng / mL standard solution, ready for use;

[0102] (2) Sample pretreatment: Accurately weigh 1.250 g of rapeseed oil sample, dissolve it with methyl tert-butyl ether / ethyl acetate (5:1, V / V), so that the concentration of the sample to be tested is 255 mg / mL , C18 solid phase extraction cartridge (1000 mg) was activated with 3 mL of methanol for 3 times, absorbed 200 μL of sample solution onto the C18 solid phase extraction column, eluted the sample with 3 mL of methanol for 3 times, and added 3 mL of n-hexane / ethyl aceta...

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Abstract

The invention discloses a method for efficiently and accurately measuring glycidyl esters in vegetable oil. The method comprises the following steps of (1) the pretreatment of a sample to be measuredincluding the steps of dissolving a vegetable oil sample to be measured by using a mixed solvent consisting of methyl tert-butyl ether and ethyl acetate, feeding the dissolved vegetable oil sample tobe measured into a solid phase extraction cartridge, eluting, blowing nitrogen and redissolving to obtain a solution to be measured, and (2) UPLC-MS/MS determination including the steps of carrying out liquid chromatography and mass spectrometry determination on the solution to be measured to obtain the content of the glycidyl esters (GEs). The detection method provided by the invention has strongoperability, good sensitivity and accurate and good reproducibility, compared with the prior art, the accurate and quantitative detection of the GEs in an actual edible vegetable oil sample can be met and the method is more suitable for application of university researchers and industrialization.

Description

technical field [0001] The invention belongs to the field of food detection, and in particular relates to a method for detecting glycidyl ester GEs in vegetable oil. Background technique [0002] Glycidyl fatty acid esters (referred to as glycidyl esters, Glycidyl Esters, GE or GEs), as a new class of chemical hazards associated with oil thermal processing, are esterification products of the reaction between glycidol and fatty acids. These substances contain a common terminal epoxy groups, but due to the different types of fatty acids, there are many different GEs. GEs is a chemical hazard associated with thermal processing. It has been determined that GEs are mainly formed in the refining process of edible oils, especially in the high-temperature deodorization process. In addition, high-temperature conditions such as frying and baking can also promote the formation of GEs. GEs widely exist in refined edible oils and oil-based foods (including infant formula, etc.). [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/34G01N30/726G01N2030/062
Inventor 刘国琴迟海李嘉辉
Owner SOUTH CHINA UNIV OF TECH
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