The invention relates to the field of biological medicines, in particular to a formula of a plant insecticidal and bactericidal agent. The plant insecticidal and bactericidal agent is prepared from the following components by weight: 5-30 parts of tobacco leaves, 50-75 parts of peach bark or peach leaves, 2-3 parts of an emulsifier, 2-4 parts of copper sulfate and 15-25 parts of auxiliary materials. A preparation method of the plant insecticidal and bactericidal agent comprises the following steps: S1, weighing; S2, cleaning of raw materials; S3, juicing of the tobacco leaves; S4, juicing of the peach bark or peach leaves; S5, extraction, S6, preparation, S7, uniform spraying of the plant insecticidal and bactericidal agent to surfaces of crops by a spraying device. The formula is simpler and more scientific and reasonable, can play a very good role in insect and bacteria killing, contains no chemical hazard ingredients, is harmless to humans and animals, can avoid chemical residues in crops, can be widely used in the insecticidal and bactericidal process of plants and has broad market prospect.