The invention provides a lignin surface sizing agent, and a preparation method of the lignin surface sizing agent. The lignin surface sizing agent is a water dispersion by copolymerizing lignin, alkali, trimethylamine, chloropropylene oxide, acid, a cation monomer, a (methyl)acrylic ester monomer, (methyl)styrene, and an initiator. The lignin surface sizing agent is suitable for surface treatmentof corrugated medium. The product and starch realize surface sizing on the corrugated medium, a Cobb value of the corrugated paper can be improved, the slaking speed is accelerated, the surface ring crush strength of the corrugated paper is greatly increased, the development requirement of the high strength corrugated paper can be adapted, in addition, during the preparation method of the lignin surface sizing agent, the lignin generated during a papermaking process is used as an initial raw material, the cost is low, the operation step is simple, and the preparation method of the lignin surface sizing agent is suitable for large-scale industrial production.