82results about How to "Short separation cycle" patented technology

The invention provides a continuous gradient elution system based on a stimulated moving bed (SMB) and a treatment method of the continuous gradient elution system. The continuous gradient elution system comprises the SMB and a matched device connected with a single chromatographic column in an elution area of the SMB, and the matched device comprises five liquid storage tanks, thirteen valves and five pumps. Gradient elution liquid can be continuously and periodically supplied to each chromatographic column in the elution area of the SMB. Each time when a turnplate of the SMB rotates by a cell, and both the gradient elution process and a mode of gradient elution liquid concentration increase with time of each chromatographic column are completely coincident with that of the previous chromatographic column, so that gradient elution of each chromatographic column in the elution area is realized. The continuous gradient elution system has good elution and separation effect; with equal elution flow, single-stage separation factor of a to-be-separated target component is greater than isocratic elution, band migration distance needed for separating to obtain a target product is short, separation period is short, process operation is simple, and programmed operation control can be realized easily.

The invention discloses a method for extracting intravenous injection human immunoglobulin by low temperature ethanol. In the method, FI, FII+III, FIII and FII are sequentially separated; and when FII precipitates are subjected to ultrafiltration and purification, processes of ultrafiltration dealcoholization, Pasteur inactivation, primary precipitation separation, secondary precipitation separation and secondary precipitation ultrafiltration dealcoholization are sequentially adopted. According to the invention, in the filtering process, the temperature of a filter press is controlled in a low-temperature range; the proper electrical conductivity is adopted in the precipitation separation processes; yield of the immunoglobulin is improved; when the immunoglobulin is purified and refined, precipitation separation is carried out for twice; secondary clarification sterilization filtration is adopted in the process of carrying out precipitation separation for twice; and by a four-stage filtering technology, insoluble particles in a product are removed and clarity and yield of the product are improved.

An internal heat exchange method of the production reaction vessel made of magnesium spongy titanium and the equipment of it. The method is as following, the heat exchanger will be inserted into the inner of the reactor, and air-blown medium will be input into the heat exchanger to cool the magnesium spongy titanium in the reactor. The above heat exchanger is tube-in -tube heat exchanger, and the air-blown medium is compressed air and compressed argon. The equipment is tube-in -tube heat exchanger, the inside tube is installed in outer tube by the ring flange, intake tube will be installed on the upside profile of the inside tube, the sihghtglass will be installed on the top of the inside tube, the return tube will be installed on the topside profile of the outer tube. The assemblage ring flange used to connect with the reactor will be installed under the joint between the outer tube and the return tube. The ring shaped charging box will be welded to the outside of the tube-in -tube heat exchanger and the upside of the assemblage ring flange, several feed openings connected with the ring charging box locate uniformly on the ring flange along the circle. The locally high temperature can be banished by this method and this equipment, and the densification of the product can be prevented. And it can also shorten the production cycle, and improve the production energy.

The invention discloses application of Eupalinolide K in preparing anticomplement drugs. The related research indicates that the Eupalinolide K has high anticomplement activity by complement classical pathway and bypass pathway and can be used for preparing anticomplement drugs.

The invention discloses a new Gelsmium elegans Benth. alkaloid compound as well as a preparation method and application thereof. In the preparation method, a target compound is separated from Gelsmium elegans Benth. by using a pH-zone-refining countercurrent chromatography and taking a total Gelsmium elegans Benth. alkaloid crude extract as a raw material and a high-speed counter-current chromatographic instrument as separation equipment; and the method has the advantages of large preparation amount, less sample loss and strong controllability, and is convenient and fast and is suitable for industrial production. By the determination of high-resolution mass spectrometry, ultraviolet spectrum, infrared spectrum, <1>H, <13>C-NMR (nuclear magnetic resonance) spectrum, <1>H-<1>HCOSY (correlation spectrometry), HSQC (heteronuclear single quantum coherence) spectrum and HMBC (heteronuclear multiple-bond correlation) spectrum, the new Gelsmium elegans Benth. alkaloid compound has the structure of a formula (I). A pharmacological test shows that the compound in the invention has dose dependence, powerful analgesic effect, less tolerance, addiction and side effect and treatment index far higher than that of total Gelsmium elegans Benth. alkaloid, can be used for preparing a novel powerful low-toxicity medicament or lead compound used for treating pain, and has definite industrial prospect.

The invention discloses a method for preparing cellulose compounds by using a cottonseed hull as a raw material, which is realized by hydrolyzing hemicellulose of cottonseed hull staple by using high-temperature and high-pressure liquid water, removing lignin through supercritical extraction, removing a small amount of impurities by using organic acid and inorganic acid and simultaneously preparing microcrystalline cellulose. The method comprises the following steps: separating a hull wrapped by cottonseed hull staple through ultrasonic treatment, and hydrolyzing hemicellulose in the cottonseed hull staple by using liquid water at high temperature and high pressure; performing supercritical CO2 extraction, and adding a right amount of assistant to remove lignin, thus obtaining coarse cellulose; and removing a small amount of impurities by using organic acid and inorganic acid, and simultaneously preparing microcrystalline cellulose, wherein the prepared cellulose can react with chloroacetic acid, sodium hydroxide, urea, 3-chloro-2-hydroxypropyl trimethyl ammonium chloride and other reagents to be prepared into carbamate cellulose, carboxymethyl cellulose, hydroxyethyl methyl cellulose and other cellulose ether compounds. The method overcomes the defect of using a large amount of acid and alkali in the existing cottonseed hull cellulose recovery, has the advantages of quick and effective hemicellulose separation, low cellulose loss, obviously shortened separation period, low energy consumption and high cellulose product purity, and has remarkable economic benefits and social benefits.

The invention provides a method for separating and preparing robinin monomer from wild chrysanthemum flowers, relates to a method for processing wild chrysanthemum flowers, and provides a high separation efficiency and high recycling rate method for separating and preparing robinin from wild chrysanthemum flowers by adopting the high-speed counter-current chromatography. The method comprises the following steps of: 1) preparing a solvent system which forms a stationary phase and a moving phase, wherein the solvent system consists of normal hexane, ethyl acetate, methanol and water; 2) filling the stationary phase by using high-speed counter-current chromatographic instrument, and adjusting the moving speed of the moving phase and the rotating speed of a host, and pumping the moving phase for balancing; 3) feeding a sample by a feed valve; and 4) collecting a target component, reducing pressure, evaporating to dryness and re-crystallizing to obtain the robinin monomer.

The invention relates to a separation and purification method of canine immunoglobulin and application of the canine immunoglobulin, and the separation and purification method comprises the step of sequentially carrying out cation exchange chromatography and hydrophobic chromatography on a centrifugal supernatant of canine plasma to obtain the canine immunoglobulin. According to the separation andpurification method of the canine immunoglobulin, disclosed by the invention, the purity of the canine immunoglobulin can be improved by creatively carrying out a specific two-step chromatography combination mode, namely cation exchange chromatography and hydrophobic chromatography, on pretreated healthy canine plasma, so that the purity of a product is higher, and the content of impure protein is lower; and the application security is greatly improved. Compared with the traditional low-temperature ethanol precipitation process, the canine immunoglobulin yield loss caused by precipitation, deep filtration and dissolution processes is reduced, and 100-1000L of plasma raw material can be treated on the chromatography scale of 100L of medium, so that the method can be completely amplified toactual production.

The invention relates to an integrated crushing device for medical use. The integrated crushing device for the medical use comprises a crushing chamber, a solid-liquid separating chamber, a grinding chamber, a solid-gas separating chamber and a recycled water treatment chamber. Through detailed design of specific arrangement modes of the crushing chamber, the solid-liquid separating chamber, the grinding chamber and the solid-gas separating chamber, the production efficiency and the quantity of a finished product is guaranteed not to be lost in a medicament crushing process. Through specific arrangement of the recycled water treatment chamber, wastewater produced in a production process is recycled and reutilized, so that zero pollution in a production process is really realized.

The invention relates to a method for preparing cannabidiol. The method comprises the following steps: separating a sample solution containing cannabidiol by adopting supercritical fluid chromatography to obtain high-purity cannabidiol, wherein a mobile phase in the supercritical fluid chromatography is supercritical carbon dioxide added with a modifier. According to the method, the high-purity cannabidiol can be efficiently and simply separated, the content of a main impurity, namely, cannabidiol, can be remarkably reduced, the purity of the cannabidiol is up to 97.59% to 99.89%, the sample loading amount is large, the separation period is short, the comprehensive yield is remarkably increased, and the organic solvent residue is low; and meanwhile, the method is suitable for separation and purification of various samples containing cannabidiol, is also suitable for industrial large-scale production and has a very wide application range.

The invention relates to a washing machine used for summer squashes. The washing machine comprises a stirring tank, a PLC and an ozone generator connected with the stirring tank. A stirring motor anda water inlet pipe are arranged on the upper end portion of a tank body of the stirring tank, and the bottom of the tank body of the stirring tank is of a cambered surface structure and is connected with a material mixing pipe. The output end of the stirring motor is connected with a stirring shaft. Stirring blades are connected to the stirring shaft. Brushes are arranged on the inner wall of thestirring tank in the circumferential direction. An overflow tank is connected to the portion, close to the upper end portion, of the wall of the tank body. A first liquid level sensor is arranged on the portion, corresponding to the inner portion of the stirring tank, of the upper end of the overflow tank. The tail end of the material mixing pipe is connected with a material mixing pump. A discharge port of the material mixing pump is connected with a water draining tank. A water collection tank is arranged under the water draining tank. A second liquid level sensor is arranged in the water collection tank, and the outer end of the bottom of the water collection tank is connected with a circulating pump. The output end of the circulating pump is connected with a circulating pipeline. The tail end of the circulating pipeline communicates with the water inlet pipe. By means of the washing machine, the washing effect and efficiency are improved, the automation degree is high, and labor issaved.

The invention discloses an MPLC (medium-pressure liquid chromatography) separation and purification method for koumine. The method is characterized by comprising the following steps: 1) preparing a loading solution and an elution solvent system; 2) filling a chromatographic column with filler; 3) adding the loading solution, performing gradient elution, collecting eluates of corresponding absorption peaks according to appearance time, detecting the eluates with high-performance liquid chromatography, combining the eluates containing the koumine, and performing reduced-pressure evaporation andethanol recrystallization to obtain a koumine monomer. The loading solution is prepared through the following steps: total gelsemium alkaloids or low-purity koumine are or is dissolved in an organic solvent, and a sample solution is filtered by an organic filter membrane. The filler of the chromatographic column is normal-phase or reverse-phase silica gel filler. Compared with the traditional column chromatography and high-speed counter-current methods, the separation and purification method for the koumine has the advantages of being simple and convenient to operate and good in controllability. Medium-pressure preparative chromatographs with different specifications can be selected for separation, the preparation amount can reach hectogram scale, and industrial manufacture requirements can be met.

The invention relates to a preparation method of high-purity paeoniflorin and albiflorin. The method includes the steps of firstly, adding smashed paeoniaceae plant raw materials containing paeoniflorin and albiflorin to an ethanol solution, conducting ultrasonic extraction, concentrating an extracting solution to obtain extract, dissolving extract with water, and conducting extraction and pressure reduction concentration to obtain extract; secondly, eluting extract with an alcohol-water solution through column chromatography with macroporous resin as filler, collecting flow components containing paeoniflorin and albiflorin, and conducting concentrating and drying to obtain coarse extract; thirdly, separating out the coarse extract through a high-speed countercurrent chromatography method, conducting online monitoring through an ultraviolet detector, collecting flow components and pressure reduction concentration, and conducting crystallizing and drying to obtain paeoniflorin and albiflorin, wherein a solvent system of the high-speed countercurrent chromatography is prepared from carbon trichloride, butyl alcohol, methyl alcohol and water. Paeoniflorin and albiflorin prepared through the method are high in product purity and good in quality, are suitable for high-speed countercurrent chromatographs of various types and can be easily and industrially amplified.

The invention discloses a method for preparing high-purity cannabidiol by combining macroporous resin enrichment with a dynamic axial compression column system, and belongs to the technical field of natural medicine preparation. The method comprises the following steps: drying floccules and leaves of industrial hemp, crushing and sieving; obtaining industrial hemp powder, heating, refluxing and extracting to prepare an upper column solution; passing through a macroporous adsorption resin chromatographic column; performing post-water elution, recovering the solvent from the eluent, concentrating to obtain a cannabidiol primary extract, adding methanol and crude silica gel, uniformly stirring, drying to obtain a loading sample, treating the loading sample by using a dynamic axial compressioncolumn system, carrying out gradient elution by using a mobile phase, concentrating the eluent, filtering, recovering the mobile phase solvent, and drying to obtain cannabidiol crystals. The method has the advantages of high yield, high cannabidiol purity and short extraction time, solves the problems of low production efficiency, low purity and long extraction time in the existing cannabidiol extraction technology, and is suitable for industrial extraction of cannabidiol.

The invention discloses a method for extracting arteannuin B from herba artemisiae annuae. The method comprises the following steps: after crushing the herba artemisiae annuae, immersing the herba artemisiae annuae with methanol to obtain an immersing solution; then carrying out crude separation through a macroporous resin column, carrying out secondary separation through a polyamide adsorption resin chromatographic column, carrying out sephadex chromatographic column separation and carrying out high performance liquid chromatographic column separation in sequence. The method disclosed by the invention has a short separation period and used solvents only comprise methanol and pure water; most of solvents and separation columns can be repeatedly utilized so that the separation cost is saved; meanwhile, the method is safe to operate and is environmentally friendly. Meanwhile, the purity of the separated arteannuin B can reach 98 percent or more and scopoletin impurities can be removed. By adopting the method disclosed by the invention, the arteannuin B with high purity is effectively extracted from the herba artemisiae annuae and the extraction efficiency is high, so that the method is suitable for large-scale production and has very strong industrial practicability.

The invention discloses a method for separating platinum and rhodium from an alkaline cyanide solution by using polystyrene-tributyl quaternary phosphonium resin (PS-BQP for short), and belongs to thetechnical field of platinum group metal separation. The method comprises the following steps: by taking the PS-BQP as an adsorbent, jointly adsorbing Pt(CN)42<-> and Rh(CN)63<-> in an alkaline cyanide solution medium with a pH being equal to 9.0 to 11.0 and at a solid-liquid ratio being 1:100 (g/mL); performing step-by-step elution on the adsorbed Pt(CN)42<-> and Rh(CN)63<-> according to a two-step elution method: firstly, using a KCI solution at a concentration of 0.5 to 1 mol/L to desorb the Rh(CN)63<-> adsorbed on the PS-BQP and then using an NH4SCN solution at a concentration of 1.0 to 1.5 mol/L to desorb the Pt(CN)42<-> adsorbed on PS-BQP, thereby achieving separation of the Pt(CN)42<-> and the Rh(CN)63<->. The method is simple in operation flow and short in separation period; the recovery rate of platinum is higher than 96.0%, and the recovery rate of ruthenium is higher than 95.0%; the adsorption capacity of the PS-BQP adsorbent is high, and the stability is good; the PS-BQP adsorbent can be repeatedly used after being regenerated by the saturated KCl solution.