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2results about How to "Short separation cycle" patented technology

Method for efficient isolation of human parainfluenza virus based on suspension MDCK cells

PendingCN122278778Ahigh titergood repeatabilityVaccine ProductionHuman Parainfluenza Virus
This invention discloses a method for efficiently isolating human parainfluenza virus based on suspended MDCK cells. By using the fully suspended passaged MDCK cell line for human parainfluenza virus isolation and culture and optimizing the inoculation process, single-generation virus amplification can be completed within 3 days, significantly shortening the cycle, improving virus isolation efficiency and success rate compared to traditional adherent culture methods, and enhancing experimental reproducibility. This method not only demonstrates good isolation and culture capabilities for the major subtypes of human parainfluenza virus, but also shows good adaptability to clinical samples from different sources and with different initial loads. In addition, this culture system has linear scale-up potential, is not limited by the surface area of ​​adherent culture, and can meet the large-scale virus seed preparation needs from clinical testing to vaccine mass production, providing key technical support for the integrated process from human parainfluenza virus isolation to vaccine production.
Owner:武汉市疾病预防控制中心(武汉市卫生监督所)

Liquid metal battery electrode material separation device and use method

PendingCN121951633Areduce edge effectsUniform electric field distributionTransportation and packagingSolid waste disposalElectrolysisElectrical battery
The invention discloses a liquid metal battery electrode material separation device, and relates to the technical field of energy storage batteries, the liquid metal battery electrode material separation device comprises a fixed seat, a reaction cavity, a corundum crucible, a temperature control assembly, an electrolysis structure, an anti-dissipation structure, a collection structure, a negative electrode extraction pipe and a positive electrode extraction pipe; the fixed seat comprises a bottom plate and a heat insulation supporting seat; the heat insulation supporting seat is mounted at the top of the bottom plate; the invention also discloses a use method of the liquid metal battery electrode material separation device. According to the liquid metal battery electrode material separation device, through cooperation of the annular anode basket and the cathode rod in the center, the electric field edge effect can be eliminated, and the electrolysis uniformity is improved; in addition, directional separation of the dual-electrode metal is achieved through efficient conduction of the Mg-stabilized zirconia diaphragm on lithium ions and efficient blocking of the Mg-stabilized zirconia diaphragm on antimony and bismuth ions, and efficient and directional separation and recovery of the high-purity positive and negative electrode metal can be achieved by combining the density difference of the positive and negative electrode metal and a designed bimetal exclusive extraction path.
Owner:WUHAN JIZHAO ENERGY STORAGE TECH CO LTD