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Continuous gradient elution system based on stimulated moving bed and treatment method of continuous gradient elution system

A technology for simulating moving bed and elution system, which is applied in the field of chemical and chemical separation, and can solve problems such as unavailable supply, long separation cycle, and small single-stage separation factor.

Active Publication Date: 2016-05-25
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But, when this invention carries out gradient elution, be to regard all chromatographic columns in the whole elution zone of simulated moving bed as a whole, carry out gradient elution to whole elution zone, promptly all chromatographic columns in elution zone Only one gradient eluent whose concentration changes with time is supplied for gradient elution, and each column in the elution area cannot be continuously and periodically repeatedly supplied with a gradient eluent whose concentration changes with time, and the separation cycle is still Longer, lower single-stage separation factor

Method used

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  • Continuous gradient elution system based on stimulated moving bed and treatment method of continuous gradient elution system
  • Continuous gradient elution system based on stimulated moving bed and treatment method of continuous gradient elution system
  • Continuous gradient elution system based on stimulated moving bed and treatment method of continuous gradient elution system

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Embodiment 1

[0065] Such as figure 1 As shown, this embodiment provides a continuous gradient elution system based on a simulated moving bed, and the system includes a simulated moving bed and an associated device connected to a single chromatographic column in the elution zone of the simulated moving bed. The supporting device comprises a first eluent storage tank 13, a second eluent storage tank 17, a pure water storage tank 15, a first eluent buffer tank 14 and a second eluent buffer tank 16, the first eluent The liquid outlet of the dehydration storage tank 13 is connected with the liquid inlets of the first eluent buffer tank 14 and the second eluent buffer tank 16 respectively through pipelines, and the liquid outlet of the pure water storage tank 15 is connected with the first eluent buffer tank 15 respectively. The liquid inlet of the eluent buffer tank 14 and the second eluent buffer tank 16 is connected by a pipeline, and the liquid outlet of the second eluent storage tank 17 is...

Embodiment 2

[0067] In this embodiment, the system described in Embodiment 1 is adopted, and the aqueous hydrochloric acid solution whose concentration increases linearly with time is used as the gradient eluent, and is combined with a simulated moving bed SMB device to realize boron isotope separation. First prepare two kinds of hydrochloric acid with different concentrations as the eluent of the experiment, wherein the hydrochloric acid with relatively high concentration is represented by eluent A, and the hydrochloric acid with relatively low concentration is represented by eluent B. The concentration of hydrochloric acid in eluent A is 1mol / L, and the concentration of hydrochloric acid in the initial state of eluent B is 0.1mol / L. Take 2F 1 =F 2 , the flow rate F of the eluent A 1 =15mL / min; flow rate F of eluent B 2 = 30 mL / min. Liquid flow F from the pure water storage tank 15 to the first eluent buffer tank 14 or the second eluent buffer tank 16 3 =30mL / min; the liquid flow F o...

Embodiment 3

[0070] In this embodiment, the system described in Embodiment 1 is adopted, and the aqueous hydrochloric acid solution whose concentration gradually increases in a concave curve with time is used as the gradient eluent, and a simulated moving bed device is used to realize boron isotope separation. First prepare two kinds of hydrochloric acid with different concentrations as the eluent of the experiment, wherein the hydrochloric acid with relatively high concentration is represented by eluent A, and the hydrochloric acid with relatively low concentration is represented by eluent B. The concentration of hydrochloric acid in eluent A is 1mol / L, and the concentration of hydrochloric acid in the initial state of eluent B is 0.1mol / L. Take 3F 1 =F 2 , the flow rate F of the eluent A 1 =10mL / min; flow rate F of eluent B 2 = 30 mL / min. Liquid flow F from the pure water storage tank 15 to the first eluent buffer tank 14 or the second eluent buffer tank 16 3 =30mL / min; the liquid f...

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Abstract

The invention provides a continuous gradient elution system based on a stimulated moving bed (SMB) and a treatment method of the continuous gradient elution system. The continuous gradient elution system comprises the SMB and a matched device connected with a single chromatographic column in an elution area of the SMB, and the matched device comprises five liquid storage tanks, thirteen valves and five pumps. Gradient elution liquid can be continuously and periodically supplied to each chromatographic column in the elution area of the SMB. Each time when a turnplate of the SMB rotates by a cell, and both the gradient elution process and a mode of gradient elution liquid concentration increase with time of each chromatographic column are completely coincident with that of the previous chromatographic column, so that gradient elution of each chromatographic column in the elution area is realized. The continuous gradient elution system has good elution and separation effect; with equal elution flow, single-stage separation factor of a to-be-separated target component is greater than isocratic elution, band migration distance needed for separating to obtain a target product is short, separation period is short, process operation is simple, and programmed operation control can be realized easily.

Description

technical field [0001] The invention belongs to the field of chemical and chemical separation, and relates to a continuous gradient elution system and a treatment method thereof, in particular to a simulated moving bed-based continuous gradient elution system and a treatment method thereof. Background technique [0002] The separation process of preparative chromatography involves two types of elution operations: isocratic elution and gradient elution. Isocratic elution means that during the elution separation process, the composition ratio and flow rate of the eluent remain constant, and the elution rates of the different components to be separated that are adsorbed on the stationary phase of the chromatographic column by the eluent are different, so that A method of eluting and separating different target components. Isocratic elution has the advantage of simple operation process, and is currently the most widely used chromatographic separation method. However, when the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D15/42B01D15/18
CPCB01D15/1821B01D15/424
Inventor 黄焜郑翰曹国祥左武斌刘会洲
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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