Method of separating preparation of volatile components from acorus tatarinowii

Abstract

The invention relates to a method of separating preparation of volatile components from acorus tatarinowii, wherein the volatile components can include monomer compounds or a composition thereof. The method comprises following steps: (A) with the volatile components as a raw material, extracting a crude volatile extract from the acorus tatarinowii; (B) dissolving the crude volatile extract in an organic solvent to prepare a sample solution for separating preparation; and (C) performing separating preparation to the solution through super-critical fluid chromatography technology to obtain the acorus tatarinowii volatile components. In the method, the acorus tatarinowii volatile components are separated and prepared through the super-critical fluid chromatography technology, and at the same time, a new approach of preparing [alpha]-asarone, [beta]-asarone and [gamma]-asarone is developed. Through the super-critical fluid chromatography technology, the [alpha]-asarone, the [beta]-asarone and the [gamma]-asarone are prepared synchronously just through simple sample injection and separation at a high purity. The method is high in yield of products, is short in separation time and is high in efficiency.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a method for separating and preparing volatile components of calamus calamus. Background technique [0002] Shichangpu is the dried rhizome of Acorusstatarinowii Schott, a perennial herb of the Araceae family. It was first recorded in "Shen Nong's Herbal Classic" and listed as a top grade. It is used for epilepsy, convulsions, febrile coma, forgetfulness, senile dementia and other diseases. It is one of the few good medicines for cardiovascular and cerebrovascular diseases. [0003] Modern pharmacological research has found that the volatile oil of Shichangpu has various pharmacological effects such as calming the nerves, anti-convulsions, improving intelligence and anti-aging, and is one of the main effective parts of Shichangpu. At present, there are few studies on the separation and preparation of volatile components of Shichangpu, especially its monomer compounds are diffic...

AI Technical Summary

Problems solved by technology

However, in view of the difficulty in preparing volatile monomeric compounds, so far, the China Institute for the Control of Pharmaceutical and Biological Products has not yet provided β-asarone and γ-asarone reference substances, and there are β-asarone reference substances sold by individual companies on the market The price of the product is high, the purity is low, and γ-asarone has not been sold. This situation directly affects the research on the medicinal materials of Shichangpu

[0005] In the prior art, there is a method of gradient elution on a silica gel column to prepare β-asarone, but the steps are cumbersome, the yield is low, and the cost is high; the prior art also discloses a "method for refining β-asarone", which requires The β-asarone with a purity of 97.89% to 99.45% can be obtained by repeated crystallization three times at different temperatures, but this method takes a long time, the operation is complicated, and the β-asarone used for refining the volatile oil of Acorus calamus is required to be relatively high. The content is not less than 60%; and the preparation method of γ-asarone has not yet been seen

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