Method for preparing glyceride rich in conjugated linoleic acid in supercritical state by enzyme method
A technology for the preparation of conjugated linoleic acid glycerides and enzymatic methods, which is applied in the field of preparing glycerides rich in conjugated linoleic acid, which can solve the problems of low transesterification rate in acidolysis, improve solubility and increase enzyme reaction rate , High stability effect
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[0006] Specific embodiment 1: Using free lipase to catalyze the transesterification reaction in a supercritical state is achieved by the following steps: 1. Add CLA and triglycerides to the reactor at a ratio of 1:1 to 5:1, and then add 1 ~10% deionized water, add 5~15% free lipase, seal the reactor and fill with CO 2 Tightly ventilate two to three times to ensure the complete replacement and removal of the air in the reactor, and at the same time put the reactor in water to test for leaks; 2. Fill the reactor to ensure that the pressure in the reactor reaches 7.5MPa~25Mpa while ensuring that the reactor is sealed. Place the reaction kettle in a constant temperature water bath and react for 24 to 72 hours at a temperature of 30 to 80°C and a rotation speed of 30 rpm to obtain acid hydrolysis products; 3. Terminate the reaction and take the reaction kettle out of the water bath to cool to room temperature. Release CO in the kettle 2 To normal pressure, add sodium sulfate to termi...
Example Embodiment
[0007] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in step 1, CLA and triglyceride are added to the reactor at a ratio of 2:1 to 4:1. The other steps are the same as in the first embodiment.
Example Embodiment
[0008] Specific embodiment three: This embodiment is different from specific embodiment one in that in step one, CLA and triglyceride are added to the reactor at a ratio of 1:1 to 5:1, and then 2 to 6% are added to the reactor. Ionized water. The other steps are the same as in the first embodiment.
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