Mass isolation method of natural sweetener Trilobatin

Abstract

The invention provides a mass isolation method of a natural sweetener Trilobatin. The method comprises the following steps: adding a crushed Trilobatin raw material into 30-90% (by volume) of ethanol according to the solid-to-liquid mass ratio of 1:10-50, carrying out thermal reflux for separation, filtering a separating medium to obtain filter residue and a filtrate, and carrying out vacuum concentration on the filtrate until there is no alcohol taste so as to obtain an isolate; dissolving the isolate by adding water, extracting with ethyl acetate, and carrying out vacuum concentration on an extract liquor to obtain an ethyl acetate extract; and separating the ethyl acetate extract by high-speed countercurrent chromatography so as to obtain the natural sweetener. The isolation method is simple and fast and has short isolation cycle. Monomers preparation amount is large, and monomers have multiple pharmacological activities.

Description

technical field

[0001] The invention belongs to the technical field of monomer separation, and in particular relates to a method for separating a large amount of natural sweetener trilobatin. Background technique

[0002] Sweeteners refer to food additives that impart sweetness to food or feed, improve food quality, and meet people's needs for food. Sweeteners generally have the advantages of high safety, good taste, high stability, and good water solubility. In order to improve the mouthfeel of food and medicine, many items must be added with appropriate amount of sweetener. Sucrose is the most commonly used sweetener, but sucrose is the carbohydrate with the highest caloric value. Excessive intake can cause diseases such as obesity, arteriosclerosis, high blood pressure, diabetes, and dental caries. Therefore, actively looking for and developing new sweetener resources has become an important research direction for scientists.

[0003] With the continuous deepening of r...

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