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Mass isolation method of natural sweetener Trilobatin

A natural sweetener, trilobatin technology, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of failure to achieve large-scale preparation and separation, low separation efficiency, etc., and shorten the separation time , large amount of preparation, and simple separation method

Active Publication Date: 2015-10-14
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for the separation of natural sweetener trilobatin in large quantities, aiming to solve the problems of low separation efficiency of existing natural sweeteners and the failure to achieve large-scale preparation and separation

Method used

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  • Mass isolation method of natural sweetener Trilobatin
  • Mass isolation method of natural sweetener Trilobatin
  • Mass isolation method of natural sweetener Trilobatin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Preparation of Duosuike sweet tea extract:

[0039] Take the dried Duosuike sweet tea leaves, and the samples are pulverized by a pulverizer. Weigh 500 g of Duosuike sweet tea powder, add 20 times the weight of ethanol solution with a volume fraction of 50%, reflux for extraction for 3 hours, and filter; repeat the above steps for the filter residue, and then extract twice, combine the filtrates, and concentrate under reduced pressure to extract Shape, Duosuike sweet tea extract 45.6g.

[0040] (2) Further purification of Duosuike sweet tea extract:

[0041] After taking 20 g of the sweet tea extract obtained in step (1) and adding an appropriate amount of water to suspend it, put it into a separatory funnel, add ethyl acetate to extract 500 mL, extract 3 times, collect the ethyl acetate extract, and rotate Concentrate and dry under reduced pressure by evaporation to obtain 8.3 g of ethyl acetate extract.

[0042] (3) trilobatin in the crude ethyl acetate isolate...

Embodiment 2

[0050] (1) Preparation of Duosuike sweet tea extract:

[0051] Take 200 g of Duosuike sweet tea powder pulverized by a pulverizer, add 50 times the weight of the ethanol solution with a volume fraction of 70%, reflux for extraction for 2.5 hours, and filter; repeat the above steps for the filter residue, and then extract twice, combine the filtrate, and depressurize Concentrate to the extract form, Duosuike sweet tea extract 21.4g.

[0052] (2) Further purification of Duosuike sweet tea extract:

[0053] After taking 10 g of the sweet tea extract obtained in step (1) and adding an appropriate amount of water to suspend it, put it into a separatory funnel, add ethyl acetate to extract 400 mL at the same time, extract 3 times, collect the ethyl acetate extract, and rotate Evaporate and concentrate to dry under reduced pressure to obtain 4.2 g of ethyl acetate extract.

[0054] (3) high-speed countercurrent chromatographic separation of trilobatin in the ethyl acetate extract:

...

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Abstract

The invention provides a mass isolation method of a natural sweetener Trilobatin. The method comprises the following steps: adding a crushed Trilobatin raw material into 30-90% (by volume) of ethanol according to the solid-to-liquid mass ratio of 1:10-50, carrying out thermal reflux for separation, filtering a separating medium to obtain filter residue and a filtrate, and carrying out vacuum concentration on the filtrate until there is no alcohol taste so as to obtain an isolate; dissolving the isolate by adding water, extracting with ethyl acetate, and carrying out vacuum concentration on an extract liquor to obtain an ethyl acetate extract; and separating the ethyl acetate extract by high-speed countercurrent chromatography so as to obtain the natural sweetener. The isolation method is simple and fast and has short isolation cycle. Monomers preparation amount is large, and monomers have multiple pharmacological activities.

Description

technical field [0001] The invention belongs to the technical field of monomer separation, and in particular relates to a method for separating a large amount of natural sweetener trilobatin. Background technique [0002] Sweeteners refer to food additives that impart sweetness to food or feed, improve food quality, and meet people's needs for food. Sweeteners generally have the advantages of high safety, good taste, high stability, and good water solubility. In order to improve the mouthfeel of food and medicine, many items must be added with appropriate amount of sweetener. Sucrose is the most commonly used sweetener, but sucrose is the carbohydrate with the highest caloric value. Excessive intake can cause diseases such as obesity, arteriosclerosis, high blood pressure, diabetes, and dental caries. Therefore, actively looking for and developing new sweetener resources has become an important research direction for scientists. [0003] With the continuous deepening of r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/203C07H1/08
Inventor 孙印石张玉伟
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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