Method for extracting arteannuin B from herba artemisiae annuae

A technology of artemisinin and artemisinin, which is applied in the field of preparation of natural medicinal chemistry, can solve problems such as difficulty in obtaining pure artemisinin, difficulty in industrial production, complex synthesis methods, etc., and achieves suitable for large-scale production and short separation cycle , High extraction efficiency

Active Publication Date: 2017-11-24
武汉天植生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the separation and preparation of artemisinin from Artemisia annua in experiments mostly use silica gel column separation method, etc., which mainly use organic solvents (such as chloroform, acetone, petroleum ether, etc.) for separation, and it is difficult to realize industrial production
Moreover, the scopoletin impurity in Artemisia annua is similar to the polarity of artemisinin, so it is difficult to separate the two, and it is difficult to obtain artemisinin with high purity.
At present, in order to obtain artemisinin with a purity of more than 98%, artemisinin is generally synthesized directly through organic synthesis, but the synthesis method is relatively complicated, and the process is difficult to control and the cost is high

Method used

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  • Method for extracting arteannuin B from herba artemisiae annuae
  • Method for extracting arteannuin B from herba artemisiae annuae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1) Take 10Kg of Artemisia annua medicinal material, crush it with a pulverizer, soak the crushed medicinal material with 20L of 100% methanol for 12 hours to obtain a soaking solution;

[0030] 2) Use the D-101 macroporous adsorption resin column to carry out rough separation of the soaking liquid in step 1), and the rough separation refers to eluting the macroporous adsorption resin with volume fractions of 70%, 80% methanol aqueous solution and 100% methanol successively Columns, the elution volumes are 2, 4 and 3 column volumes respectively, and the eluents with a combined volume fraction of 80% methanol aqueous solution are collected. The flow rate of each eluent is 2 column volumes / hour, and every 500ml is 1 elution fraction, and the target eluent is analyzed and collected by high-performance liquid chromatography (HPLC), and the obtained artemisinin enriched solution . Part of the methanol solvent was removed from the artemisinin-enriched solution by vacuum disti...

Embodiment 2

[0037] 1) Take 10Kg of Artemisia annua medicinal material, crush it with a pulverizer, soak the crushed medicinal material in 15L of 100% methanol for 12 hours to obtain soaking solution 1, then soak in 15L of 100% methanol for 12 hours to obtain soaking solution 2, and combine the soaking solutions 1, 2, to obtain the total soaking solution.

[0038] 2) Use the D-101 macroporous adsorption resin column to carry out rough separation of the soaking liquid in step 1), and the rough separation refers to eluting the macroporous adsorption resin with volume fractions of 70%, 90% methanol aqueous solution and 100% methanol successively Columns, the elution volumes are 2, 4 and 3 column volumes respectively, and the eluents with a combined volume fraction of 90% methanol aqueous solution are collected. The flow rate of each eluent is 2 column volumes / hour, and every 500ml is 1 elution fraction, and the target eluent is analyzed and collected by high-performance liquid chromatography ...

Embodiment 3

[0045] 1) Take 10Kg of Artemisia annua medicinal material, crush it with a pulverizer, soak the crushed medicinal material with 25L of 100% methanol for 24 hours to obtain a soaking solution.

[0046] 2) Use the D-101 macroporous adsorption resin column to carry out rough separation of the soaking liquid in step 1), and the rough separation refers to eluting the macroporous adsorption resin with volume fractions of 70%, 86% methanol aqueous solution and 100% methanol successively Columns, the elution volumes are 2, 4 and 3 column volumes respectively, and the eluents with a combined volume fraction of 86% methanol aqueous solution are collected. The flow rate of each eluent is 2 column volumes / hour, and every 500ml is 1 elution fraction, and the target eluent is analyzed and collected by high-performance liquid chromatography (HPLC), and the obtained artemisinin enriched solution . Dilute the artemisinin enriched solution with pure water (the volume ratio of pure water to the...

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Abstract

The invention discloses a method for extracting arteannuin B from herba artemisiae annuae. The method comprises the following steps: after crushing the herba artemisiae annuae, immersing the herba artemisiae annuae with methanol to obtain an immersing solution; then carrying out crude separation through a macroporous resin column, carrying out secondary separation through a polyamide adsorption resin chromatographic column, carrying out sephadex chromatographic column separation and carrying out high performance liquid chromatographic column separation in sequence. The method disclosed by the invention has a short separation period and used solvents only comprise methanol and pure water; most of solvents and separation columns can be repeatedly utilized so that the separation cost is saved; meanwhile, the method is safe to operate and is environmentally friendly. Meanwhile, the purity of the separated arteannuin B can reach 98 percent or more and scopoletin impurities can be removed. By adopting the method disclosed by the invention, the arteannuin B with high purity is effectively extracted from the herba artemisiae annuae and the extraction efficiency is high, so that the method is suitable for large-scale production and has very strong industrial practicability.

Description

technical field [0001] The invention relates to the field of preparation of natural medicinal chemistry, in particular to a method for extracting artemisinin from Artemisia annua. Background technique [0002] Artemisinin B, also known as artemisinin B, is a white powder, easily soluble in chloroform, dichloromethane, ethyl acetate and other solutions, and its molecular formula is C 15 h 20 o 3 , with a molecular weight of 248.3 and a CAS number of 50906-56-4, which is a sesquiterpene lactone compound that co-exists with artemisinin in the traditional Chinese medicine Artemisia annua. Artemisinin is a highly effective antimalarial drug, but artemisinin B showed a low antimalarial effect in drug screening against malaria in mice. However, in the Artemisia annua produced in some areas of my country, the content of artemisinin B is more than twice that of artemisinin. [0003] In recent years, many documents have indicated that artemisinin, artemisinic acid, and scopoletin ...

Claims

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Application Information

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IPC IPC(8): C07D493/10
CPCC07D493/10
Inventor 李良八吴杰赵苗牛亚丽谢正红
Owner 武汉天植生物技术有限公司
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