31 results about "Artemisia aucheri" patented technology

The invention provides a plant source bacteriostasis and detoxification deodorant. The plant source bacteriostasis and detoxification deodorant is prepared by the following plant materials in parts by weight: 10-15 parts of tobacco stems, 12-20 parts of Ilex latifolia Thunb stems, 2-8 parts of Helianthus tuberosus Linn leaves, 5-15 parts of ginkgo biloba, 8-16 parts of capsaicin, 1-5 parts of tanshinone, 15-25 parts of orange peel, 2-8 parts of paeoniflorin, 1-5 parts of tea seed, 8-15 parts of ginger stems, 15-35 parts of wild chrysanthemum flower, 5-20 parts of garlic, 5-15 parts of ginger, 20-35 parts of sophora alopecuroides, 5-10 parts of pine needle oil, 15-30 parts of artemisia annua, 20-50 parts of green beans, 30-60 parts of barley and 20-35 parts of corn. The plant source bacteriostasis and detoxification deodorant provided by the invention has the advantages of high biological activity, strong broad-spectrum antibacterial property, fast insect and egg killing, good environment-friendly property, safety, high efficiency and the like. The invention also provides a preparation method and application of the plant source bacteriostasis and detoxification deodorant.

The invention belongs to the field of Chinese medicine analysis and particularly relates to a content measurement method for Chinese patent medicine prepared from sweet wormwood, honeysuckle and gardenia jasminoides fruit. The method measures the content of nine ingredients including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, geniposide, secoxyloganin, isochlorogenic acid B, isochlorogenic acid A and isochlorogenic acid C in the Chinese patent medicine by reversed-phase high performance liquid chromatography, wherein the chromatographic conditions include: a chromatographic column is C18 column; methanol or acetonitrile is used as a flowing phase A; 0.05-to-1.0 percent phosphoric acid or 0.05-to-1.0 percent acetic acid is used a flowing phase B; the totalpercentage of flowing phases A and B is 100 percent; and gradient elute is performed. The content measurement method has high repeatability; and the method completely reflects the main ingredients and the change of the main ingredient content of the medicine from a qualitative prospect respectively and thus improves the level of the control over the quality of the medicine.

The invention discloses a method for planting artemisia apiacea. The method comprises the following steps that 1, seedlings are planted, wherein sowing and seedling growing are carried out on a seedbed, and after planting, the seedbed is drenched with five kilograms of decomposed human excreta or compound fertilizer per mu; 2, transplanting is carried out, wherein field pieces are ditched and ridged, two rows of plants are planted in each ridge, and row spacing*plant spacing is 0.8*0.8 m; 3, fertilization is carried out, wherein fertilization is carried out in three stages, according to the first stage, drenching is carried out with 10 to 15 jin of composite fertilizer or 0.3-percent composite fertilizer water per mu one week after transplanting, according to the second stage, fit opening is carried out, 35 jin of composite fertilizer or decomposed farmyard manure is applied per mu 15-20 days after transplanting, and according to the third stage, 50 jin of composite fertilizer or farmyard manure is applied per mu 35-45 days after transplanting; 4, field management is carried out, wherein waterlogging is drained in the rainy season; 5, pest control is carried out, wherein stem rot is prevented and treated through polysulfide suspending agents or thiophanate, and 40 percent of dimethoate and 20 percent of fenvalerate or imidacloprid mist spray are used per mu for preventing or treating aphids. The artemisia apiacea planted through the method is excellent in quality, the content of artemisinin is improved, and medical value is increased.

The invention discloses application of sweet wormwood to cultivation of pseudo-ginseng. In a cultivation process of the pseudo-ginseng, the sweet wormwood is taken as an insecticide and can be used for obviously reducing the danger to the pseudo-ginseng caused by plant diseases and insect pests, and the use amount of pesticides in a growth period of the pseudo-ginseng is reduced, so that the aim of green prevention and control is realized; meanwhile, after stems and leaves of the sweet wormwood are rotted and decomposed, nutritional ingredients directly enter pseudo-ginseng root soil to effectively provide nutrients to the growth of the pseudo-ginseng, so that the utilization of chemical fertilizers is reduced, and environment protection and pollution-free production of the pseudo-ginseng are guaranteed.

The invention discloses a black tomato floral-scented vinegar drink which is prepared from the following raw materials in parts by weight: 150-180 parts of black tomatoes, 30-40 parts of sticky rice, 10-15 parts of water chestnut powder, 20-30 parts of white sesame, 20-30 parts of sweet-scented osmanthus, 10-15 parts of honeysuckle flowers, 7-8 parts of phyllanthi fructus, 4-5 parts of ardisia japonica, 3-4 parts of astragalus membranaceus, 1-2 parts of pumpkin vines, 4-5 parts of reed rhizomes, 2-3 parts of artemisia carvifolia, 1-2 parts of flaccid knotweed herb, 10-15 parts of ferment powder, 40-50 parts of honey and a proper amount of acetic bacteria. A preparation method of the vinegar drink comprises the following steps: fermenting the raw materials such as the black tomatoes the sticky rice and the like by using the acetic bacteria to prepare raw vinegar; fermenting lemon powder, water chestnut leaves and skimmed milk by using lactobacillus acidophilus to prepare fermenting milk; adding the sweet-scented osmanthus and the honeysuckle flowers to obtain the vinegar drink. The prepared vinegar drink is unique in flavor, is sour, sweet and delicious, is rich in nutrition, and has the effects of nourishing yin, tonifying yang, maintaining beauty, keeping young, losing weight, removing fat and pressure, building bodies, improving sleep and preventing and treating cancers.

The invention belongs to the technical field of biological culture and particularly relates to a method for improving artemisinine content in sweet wormwood herb by chitosan treatment. The method comprises the following steps: firstly, planting seeds of the sweet wormwood herb in a sprouting culture medium for sprouting; secondly, transplanting sprouts of the sweet wormwood herb into a peat pearlstone culture medium; and finally, spraying the strains of the sprouts of the sweet wormwood herb by using solution of chitosan one to three days before harvesting. The method can effectively improve the artemisinine content in the sweet wormwood herb, has simple operation, does not need relatively higher early investment required in the process of improving the artemisinine content by utilizing a genetic engineering method, effectively reduces the production cost of the artemisinine and has great significance for meeting the large-scale production of the artemisinine.

The invention relates to a method for using the effective part of sweet wormwood polysaccharide to prepare drug. Wherein, said effective part is extracted from sweet wormwood, whose polysaccharide content is higher than 50% at 5000-50000 molecule weight, as well asisodulcitol, arabinose, xylose, mannose, cerebrose and amylaceum. The invention can be used to prepare the anti-cancer drug or immunity drug.

The invention relates to a heavy ion beam irradiation breeding method of artemisia carvifolia. The method comprises the following steps: (1), planting high quality seeds from Yongzhou of Human in fields of Yuzhou of Henan, and screening and reserving seeds according to yield, disease resistance, cold resistance and artemisinin content; (2), performing irradiation induced mutation on artemisia carvifolia seeds obtained in the first step through heavy ion beam, so as to obtain irradiated seeds; meanwhile taking nonirradiated artemisia carvifolia seeds obtained in the first step as a comparison;(3), plating the irradiated seeds to the fields, so as to form a first-generation group; meanwhile taking the nonirradiated artemisia carvifolia seeds as a comparison to select variant individual plants, and determining an optimal irradiation dosage; (4), selecting beneficial mutation types, and screening excellent variant strains; (5), performing identification test on the yield, quality and resistance of the excellent variant strains, observing and detecting hereditary stability an SRAP molecular marker, performing provincial region production testing and quality detection on stable strains,then performing variety technical evaluation, and declaring new varieties. The method effectively improves the breeding efficiency.

The invention relates to a special fertilizer for wheat. The special fertilizer consists of the following raw materials in parts by weight: 22-30 parts of vermicompost, 3-5 parts of calcium dihydrogen phosphate, 0.5-0.8 part of boric acid, 15-22 parts of pig manure, 12-18 parts of water hyacinth, 9-12 parts of ryegrass, 6-9 parts of fiveleaf akebia fruit, 3-6 parts of artemisia annua, 2-6 parts of euphorbia kansui, 0.5-1 part of EM strain, 2-3 parts of sulfur, 9-15 parts of folium eriobotryae and 0.5-1 part of ammonium molybdate. According to the special fertilizer for wheat, provided by the invention, the application amount of the fertilizer is reduced, and insect damage is reduced.

The invention relates to the field of pesticides and particularly relates to a composition for treating black spot of passion fruits. The composition contains the following raw materials in parts by weight: 20-30 parts of white hyacinth beans, 12-18 parts of cimicifugae foetidae, 10-20 parts of artemisia apiacea, 8-12 parts of aloe, 10-20 parts of cactus juice, 15-25 parts of cephalotaxus mannii, 8-12 parts of seaweeds, 10-20 parts of litsea cubeba, 15-25 parts of Chinese actinidia roots, 8-12 parts of herb of glabrous sarcandra, 12-18 parts of mint, 8-12 parts of paeonia lactiflora, 10-20 parts of shrubby breynia, 8-12 parts of compound amino acids and12-18 parts of compound bacteria. The curative composition provided by the invention has an obvious curative effect on black spot of passion fruits, the average general marked effective rate is greater than 93%, the average general cure rate is greater than 90%, the morbidity of black spot of passion fruits is controlled effectively, and the composition treats the black spot of passion fruits to the maximum extent, and exerts huge important meaning in treatment of black spot of passion fruits, and meanwhile, the composition overcomes defects caused by medicine treatment and medicine residues of the passion fruits are reduced.

The invention discloses an antiviral biological feed for pigs, and relates to the technical field of feed preparation. The antiviral biological feed comprises the following raw materials: 60-80 parts of corn flour, 40-60 parts of wheat bran, 20-40 parts of bean pulp, 8-10 parts of honeysuckle, 8-10 parts of fructus forsythia, 6-8 parts of honeysuckle stem, 8-10 parts of subprostrate sophora, 8-10 parts of houttuynia cordata, 6-8 parts of paris polyphylla, 6-8 parts of artemisia apiacea, 12-14 parts of root of common peony, 12-14 parts of loranthus parasiticus, 8-10 parts of cyrtomium fortune, 10-12 parts of burdock, 20-24 parts of radix isatidis 30-40 parts of raw vegetable added particles. The antiviral biological feed has the following benefits that raw materials are rich and low in price; the preparation technology is simple and convenient; the adding of the traditional Chinese medicine not only guarantees the good assimilation of live pigs in normal eating, but also has the efficacy of resisting viruses and is an exceptional biological feed; if the antiviral biological feed is used for a long time, not only is the growth speed of live pigs improved, but also the situation of diseases of pigs is solved radically at the same time.

The invention discloses a high-yield black fungus cultivation method. The high-yield black fungus cultivation method comprises the steps that ramulus mori, perilla leaves and pitaya peels are smashed to obtain a smashed material; after a cucumber extract, fishbone powder, wormcast and folium artemisiae argyi powder are evenly mixed, milk tree juice is added to obtain a first mixture, water and the smashed material are added to the first mixture, even stirring is performed, a second mixture is obtained after boiling, and then a cultivation medium is obtained through vacuum concentration; the cultivation medium is put into fungus bags, the side faces and the bottom faces of the fungus bags are punctured, the cultivation medium occupies 2 / 5 of the internal space of each fungus bag, artemisia apiacea is laid on the upper surface of the cultivation medium in each fungus bag, and the artemisia apiacea and the cultivation medium occupy 3 / 5 of the internal space of each fungus bag; black fungus strains are inoculated in the fungus bags in a sterile environment of 25 DEG C, the surfaces of the strains are covered with a layer of non-woven fabrics soaked with whitewash, and cultivation is performed in a dark environment. The high-yield black fungus cultivation method has the advantages of being rapid, high in yield, low in heavy metal content and high in quality.

The invention belongs to the technical field of material testing or analysis based on combination of HPLC (high-performance liquid chromatography) and MS (mass spectrometry) and particularly relates to a detection method for effective components in sweet wormwood herb. The detection method comprises the following steps: a sample is crushed and sieved, then ultrasonic extraction is performed, gradient elution is performed with HPLC, detection is performed with MS, and quantitative determination is performed with a peak-area external standard method. Content of artemisinin, arteannuic acid, arteannuin B, dihydroartemisinin, kaempferol, luteolin and quercetin in crude sweet wormwood herb can be measured simultaneously with the method, and the method is short in detection time, high in sensitivity, specificity, accuracy, precision and stability and good in linear relation and repeatability.

The invention discloses a method for extracting anthocyanidin from Canna edulis ker peel. The method comprises the following steps: weighing 70 to 80 parts by weight of Canna edulis ker peel, cleaningthe Canna edulis ker peel, then dehydrating the Canna edulis ker peel, and filling a fermentation tank with the dehydrated Canna edulis ker peel; adding 8 to 12 parts by weight of sweet wormwood powder and then adding 10 to 15 parts by weight of medical hydrochloric acid; carrying out a reaction for 20 to 30 h, then adding 1 to 3 parts by weight of licorice root powder, 10 to 15 parts by weight of a leaf-greening agent and 6 to 8 parts by weight of sodium sulfate dodecahydrate, carrying out uniform mixing under stirring, and then performing distillation; carrying out cooling to 15 to 40 DEG Cand adding edible alcohol for point coagulation; and extracting waste water at an upper layer after 20 to 30 h, wherein bottom precipitate juice is anthocyanidin. The method of the invention can expand the application range of the Canna edulis ker peel and increase the added value of the Canna edulis ker peel, and has good economic prospects.

The invention relates to a special fertilizer for gastrodia elata. The special fertilizer comprises the following raw materials: 2-5 parts of goat blood, 3-5 parts of phosphate diamine, 0.5-0.8 part of boric acid, 15-22 parts of agaricus bisporus, 3-5 parts of argyreia acuta, 12-15 parts of elm bark, 6-9 parts of rubia cordifolia, 3-6 parts of artemisia annua, 2-6 parts of viscum articulatum, 0.5-1 part of EM microbial strain, 2-3 parts of sulphur and 9-15 parts of mulberry leaves. The special fertilizer for gastrodia elata provided by the invention can reduce the fertilizer application dose and improve the health-care value of gastrodia elata.

The invention discloses a method for extracting a green ecological non-toxic pesticide from Canna edulis ker wastewater. The method comprises the following the steps: adding, by weight, 2 to 3 parts of zinc sulfate, 1 to 3 parts of ferrous sulfate, 1 to 2 parts of the powder of Capsicum frutescens, 3 to 5 parts of brown sugar, 6 to 8 parts of pine needle powder and 3 to 5 parts of puffed sweet wormwood powder into 90 to 100 parts of Canna edulis ker wastewater produced in the process of production of Canna edulis ker starch; carrying out uniform mixing under stirring and then performing decocting; performing residue removal via a centrifuge; and allowing juice to enter a dissolving machine for purification. The method of the invention can realize effective recycling of the Canna edulis kerwastewater, prepares the non-toxic pesticide with high added value from the Canna edulis ker wastewater and has good economic benefits; and the prepared non-toxic pesticide is applicable to killing of pests attacking plants, promotion of plant growth, etc.

The invention discloses a safe, efficient and energy-saving technological process for extracting artemisinin or artemisia annua extract. The technological process comprises the following steps: adding the leaves of sweet wormwood into a percolation tank; adding dichloromethane to completely immerse the leaves of sweet wormwood; opening a steam valve for heating; opening an ultrasonic source for ultrasonic treatment; after half hour of ultrasonic treatment, enabling the percolate in the percolation tank to enter a concentration tank for concentration. According to the technological process disclosed by the invention, by adopting non-flammable dichloromethane as a solvent and performing the ultrasonic treatment, percolation and concentration at the same time, the production safety is ensured while the solvent can be remarkably saved, and the consumption of steam and power is substantially reduced.

The invention discloses Artemisia annua L. PDR sub-family transport protein. Amino acid sequences of the protein comprise the amino acid sequence shown as SEQ ID NO:2 or the amino acid sequence of the protein is shown as SEQ ID NO:2 or nucleic acid codes crossed by the protein and complementary chains of nucleic acid of protein with the coded amino acid sequence shown in SEQ ID NO:2 under the highly-strict condition are achieved. Furthermore, the protein is named AaPDR1 by the inventor and is transport protein specifically expressed by Artemisia annua L. secreting type gladular trichomes, and it is proved through a yeast transport experiment and interference of Artemisia annua L. in the transport protein that the AaPDR1 participates in transporting an artemisinin synthetic route intermediate product dihydroartemisinic acid in Artemisia annua L.. The Artemisia annua L. PDR sub-family transport protein has great significance in providing high-yield and stable new drug resources for large-scale production of artemisinin.

The invention discloses heat clearing aloe, mung bean and red jujube wine. The heat clearing aloe, mung bean and red jujube wine comprises, by weight, 98-108 parts of red jujube, 8-10 parts of mung beans, 4-5 parts of celery, 2-3 parts of aloe, 2-3 parts of fresh citrus peel, 3-4 parts of flos sophorae, 0.7-0.9 part of sweet wormwood herb, 0.9-1.1 parts of bamboo shaving, 0.7-0.8 part of radix scutellariae, 0.4-0.5 part of bitter oranges, an appropriate quantity of whisky, an appropriate quantity of honey of lychee flowers, an appropriate quantity of pectinase and an appropriate quantity of dry yeast. The heat clearing aloe, mung bean and red jujube wine has the advantages that the red jujube is used as a main material, the mung beans and the like are used as auxiliary materials, the red jujube and the mung beans contain diversified components beneficial to human bodies, and accordingly the heat clearing aloe, mung bean and red jujube wine has excellent nutritional and medicinal value; the whisky is added into red jujube raw wine in primary fermentation and secondary fermentation procedures, accordingly, fruity flavor and wine aroma harmoniously coexist in the finished wine, and the heat clearing aloe, mung bean and red jujube wine tastes mellow, is full-bodied and has clear and transparent colors; diversified substances are soaked in the whisky, so that the heat clearing aloe, mung bean and red jujube wine contains comprehensive nutrition and has heat clearing and dryness eliminating healthcare functions.

The invention discloses antibacterial blood-pressure-reducing sea buckthorn-flavor pectin vinegar and a preparation method thereof. The pectin vinegar is prepared from the raw materials including, by weight, 1000-1100 parts of sea buckthorn fruit, 200-230 parts of soybean milk, 300-350 parts of liquor, 120-130 parts of rice bran peptide, 30-35 parts of matcha powder, 10-12 parts of flowers of kudzuvine, 10-12 parts of radix ophiopogonis, 6-7 parts of tea seed oil, 25-30 parts of stir-fried Chinese yam, 30-35 parts of dried apples, 10-12 parts of cortex moutan, 20-23 parts of edible pectin, 10-12 parts of artemisia apiacea, 10-12 parts of concha haliotidis, 10-12 parts of cape jasmine, and 30-35 parts of Chinese date honey. The antibacterial blood-pressure-reducing sea buckthorn-flavor pectin vinegar has the effects of bacteria resistance, blood pressure reduction, immunity enhancement, fatigue alleviation and heat dissipation.

The invention relates to a detecting method for a traditional Chinese medicinal preparation for treating cold. The detecting method comprises the steps of identifying characters, checking, identifying components and determining the content, wherein the component identifying step comprises the following specific steps: identifying grape-leaved anemone roots, identifying radix scutellariae, identifying radix glycyrrhizae, identifying sun-dried radix ginseng, identifying herba artemisiae scopariae and identifying herba artemisiae apiaceae; the content identifying step means content determination of baicalin. Aiming at a current detecting method that more comprehensive monitoring of effective components of the traditional Chinese medicinal preparation cannot be realized, and interfusion of the grape-leaved anemone roots as a counterfeit drug and the herba artemisiae scopariae as a confused material cannot be monitored, the invention formulates a component identifying and content determining method with scientificity, reasonability and feasibility; by using the component identifying and content determining method, clinical curative effect of a medicine is ensured, several medicinal materials are enabled to have specific quality indexes, and use of all the medicinal materials in the medicine is ensured, thereby ensuring the curative effect of the medicine; besides, the grape-leaved anemone roots as the counterfeit drug of radix bupleuri and the herba artemisiae scopariae as the confused material of the herba artemisiae apiaceae can be effectively detected, and further the harm to the health of patients caused by toxic and harmful ingredients is avoided.

The invention relates to a method of utilizing the pts gene and antisense ads to increase patchouli alcohol content in Artemisia annua L. plants. Using transgenic Artemisia annua L. plants, the method of the invention consistently increases the patchouli alcohol content in those plants, thus laying down a solid foundation for large-scale production of patchouli alcohol and other secondary metabolites such as terpenes other than artemisinin.

The invention belongs to the field of an organic fertilizer, and particularly relates to a special organic fertilizer for passion flower upgrading and a preparation method thereof. The organic fertilizer is prepared from the following ingredients in parts by weight: 60 to 100 parts of rosemary herb and sweet wormwood herb medicine preparation slag, 40 to 70 parts of mushroom slag, 30 to 62 parts of bean cake, 20 to 32 parts of oil tea cake, 12 to 30 parts of rapeseed meal, 14 to 18 parts of turf, 2 to 8 parts of humic acid, 3 to 6 parts of ammonium nitrate, 2 to 5 parts of potassium sulphate,6 to 11 parts of ammonium phosphate, 6 to 16 parts of lime, 8 to 26 parts of biological strains and 4 to 14 parts of trace element mixtures. The organic fertilizer has the advantages that the sugar degree of the passion flowers can be effectively improved; the fragrance is enhanced; the acidity is weakened; the plant disease resistance is enhanced.

The invention relates to a method suitable for cultivating artemisia annua in the northern plain area. The method comprises the following steps of (1) selecting a high-yield, high-quality, high-resistance artemisia annua variety as a good seed; (2) fully fertilizing in a field in the northern plain area with farmyard manure thoroughly decomposed, a compound fertilizer, mixing with a soil conditioner to improve the soil, and then deeply ploughing to 20-30 cm; (3) in early March each year, firstly evenly mixing mud, decomposed farmyard manure and Chinese medicine residue dry powder to form the soil, and then laying the soil into a seeding bed; (4) pouring the seeding bed with water, then mixing the good seed in step (1) with the fine soil, and spreading evenly on the seeding bed; (5) erecting a heat preservation shed on the seeding bed and covering the heat preservation shed with a plastic film; (6) waiting for that the seedling height is 10-15cm, and transplanting the seedlings in a oneridge and one row mode and conducting field planting in the field obtained in step (2); (7) when the height of the artemisia annua is 0.5-0.8 m, removing a main bud, and then applying a NPK fertilizer and spraying a borax solution when the growth of the artemisia annua is the strongest; (8) carrying out field management according to conventional methods. The method is simple and efficient.

The invention relates to a method for improving the content of artemisinin in sweet wormwood herb through transferring F3H gene. The method comprises the following steps: cloning flavanone-3-hydroxylase (F3H) gene in sweet wormwood herb to construct an F3H gene-containing plant expression vector, mediating with Agrobacterium tumefaciens, transferring the F3H gene to the sweet wormwood herb, and regenerating a plant, carrying out PCR detection on the integration condition of the exogenous target gene F3H, carrying out HPLC-ELSD detection on the content of artemisinin in transgenic sweet wormwood herb, and screening to obtain transgenic sweet wormwood herb plants with improved content of artemisinin. The content of artemisinin in the obtained transgenic sweet wormwood herb is substantially improved, and the highest content of artemisinin is 1.95 times the content of artemisinin in a nontransgenic control plant, so the method for improving content of artemisinin in sweet wormwood herb lays a foundation for large-scale production of artemisinin through using the transgenic sweet wormwood herb.

The invention relates to a hybridization breeding method of a traditional Chinese medicinal material-sweet wormwood and belongs to the technical field of genetic breeding of plants. The hybridization breeding method of the traditional Chinese medicinal material-sweet wormwood comprises the following steps: collecting different sweet wormwood sources and selecting a single plant with the high yield of sweet wormwood leaves and the high content of artemisinin; carrying out insulation and autocopuation by using a breeding bag after the pregnancy of buds and in ahead of the blooming stage, and continuously carrying out autocopuation for more than three generations; carrying out single plant tissue culture by starting from the fourth generation and carrying out hybridization combining ability tests to select male parents and female parents; and finally carrying out expanding propagation on the selected single plants obtained from tissue culture, inserting and planting the male parents and the female parents in an isolation area, carrying out natural pollination and respectively collecting seeds of the male parents and the female parents. The hybridization breeding method of the traditional Chinese medicinal material-sweet wormwood is capable of culturing different generations of sweet wormwood autocopuation systems, culturing and producing sweet wormwood hybrid seeds with the stable yield of the sweet wormwood leaves and the high content of the artemisinin; the method is simple to operate, mild in conditions and suitable for agricultural industrialization promotion.