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Method for improving artemisinine content in sweet wormwood herb by chitosan treatment

A technology of artemisinin and chitosan solution, which is applied in the directions of horticultural methods, botanical equipment and methods, fertilizer mixtures, etc. The effect of reducing production costs

Inactive Publication Date: 2010-07-28
SUZHOU TANGJI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the content of artemisinin in the callus is less than 0.1% of the dry weight, and the highest in the shoot is only 0.16% of the dry weight, and most studies have not detected artemisinin in the root, so the use of tissue culture and cell Engineering to produce artemisinin is not feasible
In addition, using genetic engineering technology to obtain new varieties of Artemisia annua with higher content of artemisinin is a more feasible method, but the use of genetic engineering to increase the content of artemisinin is still in the experimental stage

Method used

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  • Method for improving artemisinine content in sweet wormwood herb by chitosan treatment
  • Method for improving artemisinine content in sweet wormwood herb by chitosan treatment

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Embodiment

[0020] Plant the seeds of Artemisia annua (derived from the Artemisia annua species with higher artemisinin content in Youyang, Chongqing) on ​​the seedling medium to germinate the seedlings. The formula of the seedling medium is MS culture fluid, wherein 25-35g / L of Sucrose and 2-3g / L plant gel. When the Artemisia annua seedlings grow to about 5 cm, they are transplanted into the peat-perlite culture medium (the weight ratio of peat and perlite is 1:0.5-1:2), and watered once a week with ordinary nutrient solution. Wherein the common nutrient solution component is calculated by weight percentage: 0.0033% NH 4 NO 3 , 0.0038% KNO 3 , 0.00088% CaCl 2 .2H 2 O, 0.00074% MgSO 4 .7H 2 O and 0.00034% KH 2 PO 4 , and the balance is water. When the plant grows to about 40-60cm (the early flowering stage of the plant), the Artemisia annua plant is treated with chitosan solution, and the Artemisia annua plant treated with water is used as a control. Two days later, the leaves o...

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Abstract

The invention belongs to the technical field of biological culture and particularly relates to a method for improving artemisinine content in sweet wormwood herb by chitosan treatment. The method comprises the following steps: firstly, planting seeds of the sweet wormwood herb in a sprouting culture medium for sprouting; secondly, transplanting sprouts of the sweet wormwood herb into a peat pearlstone culture medium; and finally, spraying the strains of the sprouts of the sweet wormwood herb by using solution of chitosan one to three days before harvesting. The method can effectively improve the artemisinine content in the sweet wormwood herb, has simple operation, does not need relatively higher early investment required in the process of improving the artemisinine content by utilizing a genetic engineering method, effectively reduces the production cost of the artemisinine and has great significance for meeting the large-scale production of the artemisinine.

Description

technical field [0001] The invention belongs to the technical field of biological cultivation, and in particular relates to a method for increasing the content of artemisinin in Artemisia annua. Background technique [0002] Artemisinin has been recognized all over the world as an effective antimalarial drug. At present, the main source of artemisinin is extracted from the aerial parts of Artemisia annua plants. Artemisia annua L. (Qinghao), also known as Artemisia annua, is an annual herb medicinal plant. Due to the very low content of artemisinin in Artemisia annua (0.01%-1% dry weight), the large-scale commercial production of this drug is limited. Although artemisinin can be artificially synthesized now, it is not feasible for commercial production due to the difficulty, low yield and high cost. [0003] The use of tissue culture and cell engineering to produce artemisinin is an attractive approach. However, the content of artemisinin in the callus is less than 0.1% ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06A01G31/00A01G9/10C05G1/00
Inventor 唐罗欧王玥月孙小芬
Owner SUZHOU TANGJI BIOTECH CO LTD
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