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Heavy ion beam irradiation breeding method of artemisia carvifolia

A heavy ion beam and seed technology, applied in the field of Chinese herbal medicine biotechnology breeding, can solve the problems of low efficiency, long cycle, limited variation types, etc., and achieve the effect of improving a single genetic structure

Active Publication Date: 2018-10-19
INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The innovation of germplasm resources is mostly based on conventional methods, which have a long cycle and low efficiency, and the types of variations created are very limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 A method for breeding Artemisia annua heavy ion beam irradiation, comprising the following steps:

[0021] (1) The seeds of Artemisia annua with high artemisinin content, large leaves and good plant shape were collected in Yongzhou, Hunan, and the imported seeds were planted in the field of Yuzhou, Henan. The results showed that the disease resistance, yield, and artemisinin content of this variety were better than those of the local planting varieties. According to the plant type and growth performance of individual plants in the field, bad individual plants were eliminated, good individual plants were retained, and seeds with full grains were selected to be used as heavy ion beam irradiation breeding materials.

[0022] ⑵ Using the National Laboratory Lanzhou Heavy Ion Accelerator, using a heavy ion beam with an energy of 80 MeV / u and a linear energy transfer density LE T=28.12 keV / μm 20 Ne 10+ , with an irradiation dose of 10-20 Gy and a dose rate of 2 Gy...

Embodiment 2

[0027] Example 2 A method for breeding Artemisia annua heavy ion beam irradiation, comprising the following steps:

[0028] (1) The seeds of Artemisia annua with high artemisinin content, large leaves and good plant shape were collected in Yongzhou, Hunan, and the imported seeds were planted in the field of Yuzhou, Henan. The results showed that the disease resistance, yield, and artemisinin content of this variety were better than those of the local planting varieties. According to the plant type and growth performance of individual plants in the field, bad individual plants were eliminated, good individual plants were retained, and seeds with full grains were selected to be used as heavy ion beam irradiation breeding materials.

[0029] ⑵Using the National Laboratory Lanzhou Heavy Ion Accelerator, using a heavy ion beam with an energy of 120 MeV / u and a linear energy transfer density LET=28.12 keV / μm 12 C 6+ , with an irradiation dose of 60-80 Gy and a dose rate of 10 Gy / m...

Embodiment 3

[0034] Example 3 A method for breeding Artemisia annua heavy ion beam irradiation, comprising the following steps:

[0035] (1) The seeds of Artemisia annua with high artemisinin content, large leaves and good plant shape were collected in Yongzhou, Hunan, and the imported seeds were planted in the field of Yuzhou, Henan. The results showed that the disease resistance, yield, and artemisinin content of this variety were better than those of the local planting varieties. According to the plant type and growth performance of individual plants in the field, bad individual plants were eliminated, good individual plants were retained, and seeds with full grains were selected to be used as heavy ion beam irradiation breeding materials.

[0036] ⑵ Using the National Laboratory Lanzhou Heavy Ion Accelerator, using a heavy ion beam with an energy of 100 MeV / u and a linear energy transfer density of LET=28.12 keV / μm 16 o 8+ , with an irradiation dose of 20-60 Gy and a dose rate of 6 G...

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Abstract

The invention relates to a heavy ion beam irradiation breeding method of artemisia carvifolia. The method comprises the following steps: (1), planting high quality seeds from Yongzhou of Human in fields of Yuzhou of Henan, and screening and reserving seeds according to yield, disease resistance, cold resistance and artemisinin content; (2), performing irradiation induced mutation on artemisia carvifolia seeds obtained in the first step through heavy ion beam, so as to obtain irradiated seeds; meanwhile taking nonirradiated artemisia carvifolia seeds obtained in the first step as a comparison;(3), plating the irradiated seeds to the fields, so as to form a first-generation group; meanwhile taking the nonirradiated artemisia carvifolia seeds as a comparison to select variant individual plants, and determining an optimal irradiation dosage; (4), selecting beneficial mutation types, and screening excellent variant strains; (5), performing identification test on the yield, quality and resistance of the excellent variant strains, observing and detecting hereditary stability an SRAP molecular marker, performing provincial region production testing and quality detection on stable strains,then performing variety technical evaluation, and declaring new varieties. The method effectively improves the breeding efficiency.

Description

technical field [0001] The invention relates to the field of biotechnology breeding of Chinese herbal medicines, in particular to a heavy ion beam irradiation breeding method for Artemisia annua. Background technique [0002] Artemisia annua (alias: Artemisia annua, Latin name: Artemisia annua L.) is a widely distributed species in the world, but its artemisinin content varies greatly with different places of origin. Except for a few southern regions such as Guangxi, Chongqing, and Hunan in my country, the content of artemisinin in Artemisia annua grown in most parts of the world is very low (1% or less). According to reports, increasing the content of artemisinin in Artemisia annua by 1% will reduce the industrial cost by 60%~70%. Therefore, screening germplasm resources with high content is an important way to improve the quality of drug sources and reduce the cost of pharmaceuticals, and it is the material basis for breeding of Artemisia annua. [0003] The innovation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06C12Q1/6895
CPCA01H1/06C12Q1/6895
Inventor 李雪虎梁剑平陆锡宏辛志君周翔张梅彭学东
Owner INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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