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A kind of heavy ion beam irradiation breeding method of Artemisia annua

A heavy ion beam and seed technology, applied in the field of Chinese herbal medicine biotechnology breeding, can solve the problems of low efficiency, long cycle, limited variation types, etc., and achieve the effect of improving a single genetic structure

Active Publication Date: 2021-11-16
INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The innovation of germplasm resources is mostly based on conventional methods, which have a long cycle and low efficiency, and the types of variations created are very limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 A artemisian heterogeneous beam irradiation breeding method, including the following steps:

[0021] (1) Acquired Artemisiain seeds in Yongzhou, Hunan Province, and the seeds of artemisiain seeds were collected, and the seeds will introduce seeds in the fields of Henan Province. The results show that the variety of disease resistance, production, and artemisinin content is superior to local Planting varieties. According to the single plant type and long-term performance, the fine single strain is eliminated, and the fine single strain is retained, and the seeds full of seed grains are selected, as the hembladed irradiation breeding material.

[0022] (2) Using the national laboratory of Lanzhou Heavy ion plus accelerator, energy is 80 meV / U, the transfer of the transmission line density LE T = 28.12 keV / μm heavy ion beam 20 NE 10+ The irradiation of the artemon seeds obtained by the suction of Artemisia-1 GY / min for the seeding of the artemisusian seeds obta...

Embodiment 2

[0027] Example 2 An article of irradiation breeding of artemisiac ionion, including the following steps:

[0028] (1) Acquired Artemisiain seeds in Yongzhou, Hunan Province, and the seeds of artemisiain seeds were collected, and the seeds will introduce seeds in the fields of Henan Province. The results show that the variety of disease resistance, production, and artemisinin content is superior to local Planting varieties. According to the single plant type and long-term performance, the fine single strain is eliminated, and the fine single strain is retained, and the seeds full of seed grains are selected, as the hembladed irradiation breeding material.

[0029] (2) Use the national laboratory of Lanzhou Heavy ion plus accelerator, the energy of 120 meV / U, the transmission line density LET = 28.12 KEV / μm is used. 12 C 6+ The irradiation of the artemisus seeds obtained by the sediment obtained by 10 gy / min for the amount of irradiated seeds were carried out by the irradiated...

Embodiment 3

[0034] Example 3 A artemisiac ionion beam irradiation breeding method, including the following steps:

[0035] (1) Acquired Artemisiain seeds in Yongzhou, Hunan Province, and the seeds of artemisiain seeds were collected, and the seeds will introduce seeds in the fields of Henan Province. The results show that the variety of disease resistance, production, and artemisinin content is superior to local Planting varieties. According to the single plant type and long-term performance, the fine single strain is eliminated, and the fine single strain is retained, and the seeds full of seed grains are selected, as the hembladed irradiation breeding material.

[0036] (2) Using the National Laboratory Heavy ion plus accelerator, energy is 100 meV / U, transmission line density LET = 28.12 keV / μm heavy ion beam 16 O 8+ The amount of irradiation mutagenesis obtained by the artemisiain seed obtained by 6 gy / min in the expiration dose is 6 Gy / min to obtain irradiation seeds; the cym is ...

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Abstract

The present invention relates to a heavy ion beam irradiation breeding method of Artemisia annua. The method comprises the following steps: (1) The high-quality seeds introduced from Yongzhou, Hunan are planted in the field of Yuzhou, Henan, and the yield, disease resistance, cold resistance and artemisinin are combined. (2) use heavy ion beams to carry out irradiation mutagenesis on the seeds of Artemisia annua obtained in step (1) to obtain irradiated seeds; meanwhile, use the seeds of Artemisia annua obtained in step (1) without irradiation as a control; (3) irradiate The seeds were planted in the field to form the first-generation population; at the same time, using unirradiated Artemisia annua seeds as a control, the mutant individual plants were selected, and the optimal irradiation dose was determined; (4) beneficial mutation types were selected, and excellent mutant lines were screened; (5) The yield, quality, and resistance of excellent mutant strains are identified by identification tests, SRAP molecular markers are observed to detect genetic stability, and stable strains are subjected to provincial-level regional production tests and quality inspections. After that, technical identification of varieties is carried out, and new varieties are declared. The invention effectively improves the breeding efficiency.

Description

Technical field [0001] The present invention relates to the field of Chinese herbal biological breeding, and in particular, therefore, there is a method of irradiation of artemisiac ionion beam irradiation. Background technique [0002] Artemisia (alias: Yellow Artemisia, Latin Name: Artemisia Annua L.) It is the world-wide distribution, but the content of artemisinin is very different from the place where the origin is different. In addition to my country's Guangxi, Chongqing, Hunan and other small southern regions, the content of Artemisiain in the vast majority of the world is very low (1% or less). According to reports, each percentage of artemisinin content in the artemisia will reduce costs in the industry. Therefore, the modeling of screening high content is an important way to improve the quality of the drug, reducing the cost of pharmaceutical cost, and is the material foundation of artemisia. [0003] Most germplasm resources are dominated by conventional methods, long ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/06C12Q1/6895
CPCA01H1/06C12Q1/6895
Inventor 李雪虎梁剑平陆锡宏辛志君周翔张梅彭学东
Owner INST OF MODERN PHYSICS CHINESE ACADEMY OF SCI
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