The invention discloses a composite nanometer
oxygen sensing material capable of performing efficiently
energy transfer, a preparation method and applications thereof, and belongs to the technical field of biological dissolved
oxygen content
fluorescence detection materials. According to the present invention, an amphiphilic
silane-coated composite nanometer material loading
platinum (II) MESO-
tetra(pentafluorophenyl)porphine (PtTFPP),
coumarin 6 (C6) and Fe3O4 nanoparticlses is prepared, wherein the
red light emission intensity of the
oxygen probe PtTFPP is increased through the
resonance-based
energy transfer due to the loading of the
coumarin 6 (C6), such that the oxygen sensitivity is improved compared to the absence of the
coumarin 6 (C6); by using the excitation of the light with thelong
wavelength of 458 nm, the disadvantages of high
noise,
biological tissue injury and the like of the
ultraviolet excitation can be reduced; the emission peak of C6 at 500 nm is not changed alongwith the change of the
oxygen content while the emission peak of PtTFPP at 652 nm is weakened along with the increase of the
oxygen content, such that the
oxygen monitoring accuracy can be improved byusing the ratio
fluorescence method; and by loading the Fe3O4 nanoparticlses, the functions of magnetic navigation, magnetic enrichment and the like can be additionally provided.