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Composite nanometer oxygen sensing material capable of performing efficiently energy transfer, preparation method and applications thereof

An energy transfer and oxygen sensing technology, applied in the field of composite nano-oxygen sensing materials, can solve the problems of weak detection fluorescence and low sensitivity, and achieve the effect of improving sensitivity and great application value

Inactive Publication Date: 2018-01-19
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at present, its shortcomings in application are weak detection of fluorescence and low sensitivity, so the development of high-intensity fluorescent oxygen sensors is the direction of most scientific researchers in recent years.

Method used

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  • Composite nanometer oxygen sensing material capable of performing efficiently energy transfer, preparation method and applications thereof
  • Composite nanometer oxygen sensing material capable of performing efficiently energy transfer, preparation method and applications thereof
  • Composite nanometer oxygen sensing material capable of performing efficiently energy transfer, preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Composite nano-oxygen sensing material (Fe 3 o 4 @PtTFPP / C6@silane) Preparation

[0026] (1) Iron ferric oxide nanoparticles were synthesized according to the co-precipitation method. In order to increase its stability, the surface was coated with oleic acid (Wang, J., et al. Remote manipulation of micronomachines containing magnetic nanoparticles. Optics Letters 34,581 -583 (2009)).

[0027] (2) The prepared oily iron ferric oxide nanoparticles are formulated into 5 mg / mL tetrahydrofuran solution, n-octyltrimethoxysilane (purchased from sigma company) is formulated into 7.5 mg / mL tetrahydrofuran solution, platinum ( Ⅱ) MESO-tetrakis(pentafluorobenzene) porphine (PtTFPP) (purchased from sigma company), coumarin 6 (C6) (purchased from sigma company) were formulated into 1mg / mL tetrahydrofuran solution respectively; Platinum (II) MESO-tetrakis(pentafluorobenzene) porphine (PtTFPP, 1mg / mL), coumarin 6 (C6, 1mg / mL), and amphiphilic silane (7.5mg / mL) were placed on a magn...

Embodiment 2

[0031] Embodiment 2 comparative example

[0032] Composite nano-oxygen sensing material (Fe 3 o 4 @PtTFPP@silane) Preparation

[0033] Repeat Example 1, the difference is that the functional oily molecule loaded this time does not contain coumarin 6 (C6) with fluorescent labeling function (purchased from sigma company). All the other steps are the same as in Example 1. The resulting product concentration was 0.168 mg / mL.

[0034] like figure 1 (a)(b):Fe 3 o 4 @PtTFPP / C6@silane NPs are well dispersed, with a thin transparent coating on the outer layer, and individual nanoparticles are spherical in shape with a diameter of about 100 nanometers. It can be seen that it is composed of many small ferric oxide nanoparticles. This is because ferric oxide nanoparticles will attract each other and agglomerate.

[0035] like figure 2 , according to the preparation method in Example 1, first determine the optimum concentration of PtTFPP, based on four different concentrations o...

Embodiment 3

[0043] Fe 3 o 4 @PtTFPP / C6 @silane and Fe 3 o 4 @PtTFPP @silane oxygen sensing test

[0044] Excite Fe using 458nm excitation light 3 o 4 When @PtTFPP / C6@silane composites nanomaterials, because it contains platinum (Ⅱ) MESO-tetrakis(pentafluorobenzene) porphine, it will emit red light, and its peak position is at 652nm, and the intensity value of the emitted light varies with changes with the surrounding oxygen content. However, coumarin 6 (C6) emits green light, and its peak position is at 500nm, and the intensity of the emitted light does not change with the change of the surrounding oxygen content. When using a gas mixer to configure mixed gases with different oxygen contents (volume fractions of oxygen are 0, 20, 40, 60, 80, 100% respectively), pass it into the sealed cuvette, each time Continue for 20 minutes, so that the gas in the cuvette reaches an equilibrium state. Finally, the composite nanomaterial in the cuvette is tested for fluorescence emission spectru...

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Abstract

The invention discloses a composite nanometer oxygen sensing material capable of performing efficiently energy transfer, a preparation method and applications thereof, and belongs to the technical field of biological dissolved oxygen content fluorescence detection materials. According to the present invention, an amphiphilic silane-coated composite nanometer material loading platinum (II) MESO-tetra(pentafluorophenyl)porphine (PtTFPP), coumarin 6 (C6) and Fe3O4 nanoparticlses is prepared, wherein the red light emission intensity of the oxygen probe PtTFPP is increased through the resonance-based energy transfer due to the loading of the coumarin 6 (C6), such that the oxygen sensitivity is improved compared to the absence of the coumarin 6 (C6); by using the excitation of the light with thelong wavelength of 458 nm, the disadvantages of high noise, biological tissue injury and the like of the ultraviolet excitation can be reduced; the emission peak of C6 at 500 nm is not changed alongwith the change of the oxygen content while the emission peak of PtTFPP at 652 nm is weakened along with the increase of the oxygen content, such that the oxygen monitoring accuracy can be improved byusing the ratio fluorescence method; and by loading the Fe3O4 nanoparticlses, the functions of magnetic navigation, magnetic enrichment and the like can be additionally provided.

Description

technical field [0001] The invention belongs to the technical field of fluorescence detection materials for biological dissolved oxygen, and in particular relates to a composite nanometer oxygen sensing material with energy transfer phenomenon, a preparation method and an application thereof. Background technique [0002] Oxygen content at the body fluid and cell levels is an important basis for understanding the metabolism of cells and tissues, and is of great significance in the field of biomedicine. The amount of dissolved oxygen in the body usually fluctuates within a certain range. If the oxygen content in the body is too low for a long time, it will lead to the occurrence of some chronic diseases, such as cancer. Therefore, the level of oxygen in human cells is regarded as an information sign of whether the physiological process and metabolism of the human body are normal or not, and its quantitative detection is of great significance for the prevention and timely dete...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 董彪林艳霞孙娇李春赫徐琳白雪宋宏伟
Owner JILIN UNIV
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