Method for treating and increasing arteannuin content in sweet wormwood through carbonyl chloride (CoC12)

Abstract

The invention discloses a method for treating and increasing arteannuin content in sweet wormwood through carbonyl chloride (CoC12). The method is characterized in that seeds of sweet wormwood are planted into a seedling sprouting culture medium for sprouting, and then sweet wormwood seedlings are transplanted into a peat perlite culture medium and are irrigated with a common nutrient solution once per week, in the earlier stage of flowering, a CoC12 solution is used for treating the sweet wormwood, and then the sweet wormwood is collected on schedule. The method can effectively improve the arteannuin content in the sweet wormwood, is simple in operation, and free of higher earlier stage investment demanded by a genetic engineering method, effectively lowers production cost of arteannuin, and has significance for meeting arteannuin medical industry mass production.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, specifically, CoCl 2 Disclosed is a method for increasing the content of artemisinin in Artemisia annua. Background technique [0002] Artemisinin has been recognized worldwide as an effective antimalarial drug. At present, the main source of artemisinin is extracted from the aerial parts of Artemisia annua plants. Artemisia annua L. (Annua), also known as Artemisia annua, is an annual herbal medicinal plant. Due to the relatively low content of artemisinin in Artemisia annua (0.01%-1% dry weight), the large-scale commercial production of this drug is limited. Although artemisinin can be artificially synthesized now, it is not feasible for commercial production due to the difficulty, low yield and high cost. [0003] The use of tissue culture and cell engineering to produce artemisinin is an attractive approach. However, the content of artemisinin in the callus is less than 0.1% of th...

AI Technical Summary

Problems solved by technology

However, the content of artemisinin in the callus is less than 0.1% of the dry weight, and in the shoot, the highest content is only 0.16% of the dry weight, and most studies have not detected artemisinin in the root, so the use of tissue culture and cell engineering technology to produce artemisinin is not feasible

In addition, it is a more feasible method to use genetic engineering technology to obtain new varieties of Artemisia annua with higher artemisinin content. However, the use of genetic engineering to increase the content of artemisinin is still in the experimental stage, and the entire technology is not yet mature.

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