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Effective part of sweet wormwood, its preparation method and application thereof in pharmacy

An effective part, Artemisia annua technology, applied in the effective part of Artemisia annua polysaccharide and its preparation field, can solve the problem that there are no research reports on polysaccharide components, achieve significant immune promotion effect, simple and easy preparation method, and low acute toxicity small effect

Active Publication Date: 2008-09-10
JINLING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on Artemisia annua mainly focuses on the terpenoid components such as artemisinin and its volatile oil, and there is no research report on the polysaccharide components contained in it.

Method used

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  • Effective part of sweet wormwood, its preparation method and application thereof in pharmacy
  • Effective part of sweet wormwood, its preparation method and application thereof in pharmacy
  • Effective part of sweet wormwood, its preparation method and application thereof in pharmacy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0270] Example 1: Preparation of effective fractions of Artemisia annua polysaccharides

[0271] 1 Extraction of crude polysaccharides from Artemisia annua

[0272] Take Artemisia annua and add water to decoct 3 times, each time add 15 times the weight of Artemisia annua, 1.5 hours each time, combine the extracts, concentrate under reduced pressure, add ethanol to precipitate until the alcohol content is 85%, let stand, and filter the precipitate , washed with ethanol, and dried to obtain the crude polysaccharide of Artemisia annua,

[0273] 2 Refining of the effective fraction of Artemisia annua polysaccharide

[0274] Take the crude polysaccharide of Artemisia annua, fully dissolve it in water, the concentration is 07-1%, and use a dialysis membrane with a cut-off value of 5000 to dialyze with pure water for 72 hours to remove small molecular impurities with a molecular weight below 5000. Concentrate the sugar solution at a high speed of 15000 rpm Centrifuge for 18 minutes...

Embodiment 2

[0275] Example 2: Separation and purification of effective fractions of Artemisia annua polysaccharides

[0276] Take the effective part of Artemisia annua polysaccharide prepared according to Example 1, dissolve it in an appropriate amount of water, put it on a DEAE Sepharose FF. The positive eluate was concentrated and dialyzed with pure water for 72 hours using a dialysis membrane with a cut-off value of 5000 to remove small molecular impurities with a molecular weight below 5000. The sugar solution was concentrated under reduced pressure at 48°C and freeze-dried to obtain a powder, which is purified There are 6 effective parts of Artemisia annua polysaccharide, water, 0 1, 0 2, 0 4, 0.6, 1 0mol / L NaCl elution parts. The sugar content was determined by the sulfuric acid-phenol method, and glucose was used as the reference substance. The results showed that the sugar content of each elution site was 958%, 85.0%, 78.5%, 70.1%, 40.1%, and 20.3%. The molecular weight distribut...

Embodiment 3

[0277] Example 3: Preparation of artemisinin polysaccharide injection

[0278] The Artemisia annua polysaccharide HQG prepared according to the method of Example 1 was added into the conventional excipients for injection, prepared into 1000ml with sterilized water for injection according to the conventional process for injection, filtered aseptically, and filled.

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Abstract

The invention relates to a method for using the effective part of sweet wormwood polysaccharide to prepare drug. Wherein, said effective part is extracted from sweet wormwood, whose polysaccharide content is higher than 50% at 5000-50000 molecule weight, as well asisodulcitol, arabinose, xylose, mannose, cerebrose and amylaceum. The invention can be used to prepare the anti-cancer drug or immunity drug.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy, and relates to the effective part of Artemisia annua polysaccharide, its preparation method and its application in pharmacy. Background technique [0002] Artemisia annua is the dry aerial part of Artemisia annua L. It is a commonly used traditional Chinese medicine, which has the functions of clearing away heat and relieving summer heat, removing steam, and cutting off malaria. At present, the research on Artemisia annua mainly focuses on the terpenoid components such as artemisinin and its volatile oil, but there is no research report on the polysaccharide components contained in it. Contents of the invention [0003] The object of the present invention is to provide an effective fraction of artemisinin polysaccharide with anti-tumor effect and immune regulation effect. [0004] Another object of the present invention is to provide a method for preparing the above-mentio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61K36/282A61P35/00A61P37/02C08B37/00
Inventor 薛明田丽娟徐向阳孙晔曹春陵
Owner JINLING PHARMA
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