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Detection method for effective components in sweet wormwood herb

A detection method and technology for active ingredients, applied in the field of detection of active ingredients in Artemisia annua, can solve the problems of long detection time, low sensitivity, inability to measure artemisinin at the same time, etc., and achieve the effects of short detection time and high sensitivity

Active Publication Date: 2018-08-03
CHONGQING ACAD OF CHINESE MATERIA MEDICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, pre-column derivatization-HPLC and HPLC-UV can not simultaneously determine the content of artemisinin, artemisinin, artemisinin, dihydroartemisinin, kaempferol, luteolin and quercetin; HPLC-ELSD and The detection time of HPLC-UV-ELSD is long, it takes 30-60min, the sensitivity is low, and the components with content below 1 / 100,000 cannot be detected

Method used

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  • Detection method for effective components in sweet wormwood herb
  • Detection method for effective components in sweet wormwood herb
  • Detection method for effective components in sweet wormwood herb

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The detection method of active ingredient in the Artemisia annua of embodiment 1, concrete steps are:

[0043] A. After pulverizing samples of Artemisia annua medicinal materials (as shown in Table 1) from different places of production in various districts and counties in Chongqing, pass through No. 5 sieve of "Pharmacopoeia of the People's Republic of China" (2015 edition), and then accurately weigh 3 parts of Artemisia annua samples of 0.50g, Wrapped in filter paper, placed in 25mL measuring bottles, add 50% petroleum ether (the boiling point of petroleum ether is 60-90°C) -50% acetone (both volume ratio) extraction solvent 20mL, ultrasonic extraction under the condition of power 300W 45min, continuous ultrasonic extraction twice, combined extracts, then dilute to 100mL with 50% petroleum ether (the boiling point of petroleum ether is 60-90°C)-50% acetone (both volume ratio) extraction solvent, shake well, Take 0.1mL, put it in a 100mL measuring bottle, evaporate to ...

Embodiment 2

[0068] Embodiment 2 specificity analysis

[0069] A. After pulverizing samples of Artemisia annua from Xinli in Zhongxian County, pass through No. 5 sieve of "Pharmacopoeia of the People's Republic of China" (2015 Edition), then accurately weigh 3 parts of Artemisia annua samples of 0.50g, wrap them in filter paper, and place them in 25mL volumes respectively. In the bottle, add 50% petroleum ether (the boiling point of petroleum ether is 60-90 ℃)-50% acetone (both volume ratio) extraction solvent 20mL, under the condition of power 300W ultrasonic extraction 45min, continuous ultrasonic extraction 2 times, Combine the extracts, then use 50% petroleum ether (the boiling point of petroleum ether is 60-90°C)-50% acetone (both in volume ratio) as an extraction solvent to set the volume to 100mL, shake well, take 0.1mL, and put it in a 100mL volume In the bottle, evaporate to dryness, dissolve with methanol, and adjust the volume to the mark, shake well, filter with a 0.22 μm filte...

Embodiment 3

[0074] Embodiment 3 precision test

[0075] Draw the obtained artemisinin reference substance solution, artemisinin reference substance solution, artemisinin reference substance solution, dihydroartemisinin reference substance solution, kaempferol reference substance solution, luteolin reference substance solution and quercetin reference substance solution obtained in Example 2. Each 2 μ L of corticosteroid reference substance solution was injected into an ultra-high performance liquid chromatograph for gradient elution, and then detected by mass spectrometry. The chromatographic conditions and mass spectrometry conditions were the same as in Example 1. Continuous sampling was performed for 6 times, and the concentration of each reference substance was measured. Peak area, and calculate the relative standard deviation, the results are shown in Table 5.

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Abstract

The invention belongs to the technical field of material testing or analysis based on combination of HPLC (high-performance liquid chromatography) and MS (mass spectrometry) and particularly relates to a detection method for effective components in sweet wormwood herb. The detection method comprises the following steps: a sample is crushed and sieved, then ultrasonic extraction is performed, gradient elution is performed with HPLC, detection is performed with MS, and quantitative determination is performed with a peak-area external standard method. Content of artemisinin, arteannuic acid, arteannuin B, dihydroartemisinin, kaempferol, luteolin and quercetin in crude sweet wormwood herb can be measured simultaneously with the method, and the method is short in detection time, high in sensitivity, specificity, accuracy, precision and stability and good in linear relation and repeatability.

Description

technical field [0001] The invention belongs to the technical field of combining ultra-high performance liquid chromatography and mass spectrometry to test or analyze materials, and in particular relates to a detection method for effective components in Artemisia annua. Background technique [0002] Artemisia annua is the dried aboveground part of the annual herb Artemisia annua L. of Compositae. It is bitter in nature and pungent in coldness. Pharmacopoeia (Part One), edited by the National Pharmacopoeia Committee, Beijing: China Medical Science and Technology Press, 2015, p. 198, published on December 31, 2015). Artemisia annua is widely distributed, and it is distributed in Chongqing, Sichuan, Guizhou, Guangxi, Guangdong, Jiangxi, Hubei, Hunan, Jiangsu, Hebei, Henan, Shandong and Shaanxi ("Discussion on the analysis and evaluation methods of suitable habitats of Artemisia annua", Liu Junming et al. Agricultural Resources and Regional Planning in China, Vol. 27, No. 4, 20...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8809
Inventor 丁刚宋旭红李隆云谭均崔广林
Owner CHONGQING ACAD OF CHINESE MATERIA MEDICA
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