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A detection method for prepackaged products using unqualified fish meat raw materials

A detection method and pre-packaging technology, which are applied in the direction of chemical reaction of materials for analysis, analysis of materials, measurement devices, etc., can solve problems such as inability to effectively judge pre-packaged food and fish raw materials without perfect detection mechanisms and traceability methods, etc. Achieve the effect of low harm and strong specificity that is conducive to daily supervision and management, experimental environment and personnel

Active Publication Date: 2022-07-15
广东江门中医药职业学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no perfect detection mechanism and traceability method for the fish raw materials of prepackaged fish products. The existing detection methods cannot effectively judge whether the fish raw materials of prepackaged foods are dead fish poisoned by ammonia nitrogen. How to establish a simple, fast and low-cost The method of detection and traceability judgment is the focus and difficulty of current food safety analysis

Method used

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  • A detection method for prepackaged products using unqualified fish meat raw materials
  • A detection method for prepackaged products using unqualified fish meat raw materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] In this example, n-hexane is selected from optically pure n-hexane supplied by Sinopharm Group Chemical Reagent Co., Ltd., hydrochloric acid solution is selected from chemically pure 36% hydrochloric acid solution supplied by Sinopharm Group Chemical Reagent Co., Ltd., and sodium hydroxide is selected from Sinopharm Group Chemical Reagent Co., Ltd. Analytical pure sodium hydroxide, p-dimethylaminobenzaldehyde selected analytical pure p-dimethylaminobenzaldehyde supplied by Shanghai McLean Biochemical Technology Co., Ltd., methanol selected analytical pure methanol supplied by Sinopharm Chemical Reagent Co., Ltd., and mercury potassium iodide selected Chemically pure potassium mercuric iodide supplied by Sinopharm Chemical Reagent Co., Ltd., potassium hydroxide selected from analytical pure potassium hydroxide supplied by Sinopharm Chemical Reagent Co., Ltd., and silver nitrate selected analytically pure silver nitrate supplied by Sinopharm Chemical Reagent Co., Ltd.; lemo...

Embodiment 2

[0056] A method for detecting the use of unqualified fish meat raw materials for a prepackaged product of the present invention includes the following:

[0057] Step a, select the sample to be tested:

[0058] The finished product of canned fish and meat produced within five natural days before the sampling day is selected as the sample to be tested;

[0059] Step b, preparation of sample solution to be tested:

[0060] Weigh 5g ± 0.5g of the homogenized sample to be tested in a 50ml centrifuge tube, homogenize, add 5ml n-ethane, shake and mix for 2min, add 10ml of water, shake for 1min, and centrifuge at 4000rpm for 3min. The upper n-hexane solution layer is used to obtain the first sample solution to be tested, and the lower aqueous solution layer is taken to obtain the second sample solution to be tested;

[0061] Step c, the first test sample liquid detection:

[0062] Dissolve 1 g of p-dimethylaminobenzaldehyde in 25 ml of hydrochloric acid solution, add 75 ml of metha...

Embodiment 3

[0073] A method for detecting the use of unqualified fish meat raw materials for a prepackaged product of the present invention includes the following:

[0074] Step a, select the sample to be tested:

[0075] Select the semi-finished fish balls to be packaged produced within five natural days before the sampling day as the samples to be tested;

[0076] Step b, preparation of sample solution to be tested:

[0077] Weigh 5g ± 0.5g of the homogenized sample to be tested in a 50ml centrifuge tube, homogenize, add 5ml of n-ethane, shake and mix for 2 minutes, add 10ml of water and shake for 1 minute, then centrifuge at 5000rpm for 3 minutes. The upper n-hexane solution layer is used to obtain the first sample solution to be tested, and the lower aqueous solution layer is taken to obtain the second sample solution to be tested;

[0078] Step c, the first test sample liquid detection:

[0079]Dissolve 1 g of p-dimethylaminobenzaldehyde in 25 ml of hydrochloric acid solution, add...

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Abstract

The invention discloses a method for detecting unqualified fish meat raw materials used in prepackaged products, comprising the following steps: step a, selecting a sample to be tested; step b, preparing a sample liquid to be tested; step c, detecting the first sample liquid to be tested; d, the first standard curve is prepared; step e, the second sample liquid to be tested is detected; step f, the second standard curve is prepared; step g, the result is determined. The analysis method of this detection method is simple, rapid, highly specific, high sensitivity, obvious phenomenon, does not need large analytical instruments, does not need to use dangerous reagents such as highly toxic or carcinogenic, and has low harm to the experimental environment and personnel. Whether the raw materials used for packaging fish meat products are qualified, whether it is the fish meat with ammonia nitrogen poisoning or corrupted and stale fish meat, it is convenient to trace the source of the problem products and facilitate the daily supervision and management of prepackaged fish meat products.

Description

technical field [0001] The invention relates to the field of agricultural product detection, in particular to a detection method for prepackaged products using unqualified fish meat raw materials. Background technique [0002] As we all know, two of the detection indicators related to the aquaculture industry are of relatively high concern, namely ammonia nitrogen content and volatile base nitrogen content. [0003] Ammonia nitrogen refers to the compound nitrogen that exists in the form of ammonia or ammonium ions, that is, free ammonia (NH) in water. 3 ) and ammonium ions (NH 4 + ) in the form of nitrogen. When the ammonia nitrogen in the water exists in the form of molecular ammonia and ionic ammonia, molecular ammonia is very toxic, while ionic ammonia is non-toxic. High ammonia nitrogen usually means that the content of molecular ammonia increases. At this time, the pH value of the water body is generally the same. relatively high. If the pH value in the water body...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N1/28G01N21/31
CPCG01N21/78G01N21/31G01N1/286
Inventor 李拥军谢培泉黄伟棠刘崇温宝莹莫婷筠余廉柱
Owner 广东江门中医药职业学院
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