Detection method of free and combined carboxy methyl lysine in milk and dairy products

A technology of carboxymethyl lysine and a detection method, which is applied in the field of isotope dilution high performance liquid chromatography tandem ion trap mass spectrometry, can solve the problems of increasing retention time, damage to mass spectrometry instruments, narrow use range and the like, so as to improve quantitative accuracy , the effect of increasing the linear range and saving usage

A technology of carboxymethyl lysine and a detection method, which is applied in the field of isotope dilution high performance liquid chromatography tandem ion trap mass spectrometry, can solve the problems of increasing retention time, damage to mass spectrometry instruments, narrow use range and the like, so as to improve quantitative accuracy , the effect of increasing the linear range and saving usage

CN104965040AActive Publication Date: 2015-10-07WUHAN POLYTECHNIC UNIVERSITY
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  • Detection method of free and combined carboxy methyl lysine in milk and dairy products
  • Detection method of free and combined carboxy methyl lysine in milk and dairy products
  • Detection method of free and combined carboxy methyl lysine in milk and dairy products

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[0028] In order to better understand the technical solution of the present invention, the technical solution provided by the present invention is described in detail below in conjunction with examples, but the present invention is not limited in any way.

[0029] 1. Sample pretreatment: Accurately weigh 0.1g of solid milk powder sample, add 1mL of ultrapure water and vortex to mix to make a sample solution, directly measure 1mL of liquid dairy products; add 2-3mL of n-hexane to the sample preparation solution , after shaking well, centrifuge at 4000r / min for 10min, discard the n-hexane layer, weigh 2-3 times to completely remove fat, skim milk and milk powder can skip this step; add 1.5mL of 0.2mol / L, pH9.4 Sodium borate buffer solution, 1mL sodium borohydride solution, react at 4°C for 6h; add 60% trichloroacetic acid solution twice the volume of the sample, centrifuge at 8000r / min, 4°C for 10min, separate supernatant and protein precipitate, collect separately and wait for F...

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Abstract

The invention discloses a detection method of free and combined carboxy methyl lysine in milk and dairy products. According to the detection method, isotope dilution high performance liquid chromatography- tandem ion trap mass spectrometry is adopted to detect free carboxy methyl lysine and carboxy methyl lysine combined with protein in milk and dairy products. According to the detection method, using of ion-pairing agent is avoided; no derivatization pretreatment is needed; efficiency, accuracy, sensitivity, and reproducibility are high; linearity range is wide; the detection method possesses unique features in analysis of the content of free carboxy methyl lysine and carboxy methyl lysine combined with protein in milk and dairy products, can be used for rapid and accurate qualitative and quantitative analysis on free carboxy methyl lysine and carboxy methyl lysine combined with protein in milk and dairy products, and can be used for detection of free and combined carboxy methyl lysine in milk and dairy products preferably.

Description

technical field [0001] The invention relates to the technical field of food detection and analysis, in particular to an isotope dilution high performance liquid chromatography tandem ion trap mass spectrometry for detecting free and protein-bound carboxymethyllysine in milk and dairy products. Background technique [0002] During food processing, the Maillard reaction is prone to produce harmful substances represented by glycation end products (AGEs). Carboxymethyllysine, as a well-defined marker of AGEs, widely exists in food. [0003] Lysine has an ε-amino group, which can easily become a highly reactive carbonyl target, so foods and biological tissues rich in lysine are more prone to Maillard reactions. As a protein-rich food, dairy products are not only rich in lysine, but also high in reducing lactose (lactose is more likely to react with lysine than glucose and maltose to form carboxymethyl lysine. acid). Numerous studies have shown that carboxymethyl lysine produce...

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Application Information

Patent Timeline
07 Oct 2015
Publication
CN104965040A
IPC
G01N30/88; G01N30/06
Inventors
宫智勇; 柳鑫