38 results about "Ne carboxymethyl lysine" patented technology

The invention relates to the technical field of analysis and detection, and provides a method for synchronously detecting carboxymethyl lysine and carboxyethyl lysine in green tea in order to solve the problem that an existing method for detecting CML and CEL will be affected by different raw material substrates. d4-CML and d4-CEL are used as interior labels, a multi-choice reaction monitoring mode is adopted, a quantitative analysis method for synchronously detecting CML and CEL in green tea is built through methodological argumentation, and a foundation is provided for monitoring and controlling AGEs in tea. The detection method is good in accuracy, reproducibility and stability, and applicable to practical detection of CML and CEL in green tea.

The invention discloses a detecting method of carboxymethyl lysine (CML). The method is characterized by measuring the content of the CML in food adopting high performance liquid chromatography-tandem mass spectrometry in combination with an HILIC (Hydrophilic Interaction Liquid Chromatography) column for strong-polarity CML separation under a higher organic phase proportion. Crushed food passes through an MCX (Mixed-Mode Cation-Exchange) small column for being extracted and purified after being reduced by sodium borohydride and hydrolyzed by hydrochloric acid; sample liquor is subjected to gradient elution in the HILIC column by taking methanol-water (containing 2 mM ammonium acetate and 0.1% formic acid) as a mobile phase, so that CML is separated from a sample substrate; tandem mass spectrum analysis is carried out by using an electrospray ion source, positive ion scanning and a multi-reaction monitoring mode; quantitative analysis is carried out by using D4-CML as an internal standard substance, wherein analysis time is 12 minutes, and CML has an absorption peak at the retention time of 3.47 minutes. The detecting method disclosed by the invention is high in sensitivity, high in accuracy and good in precision, does not need to carry out derivatization onto samples, simplifies sample pre-treatment, shortens detecting time and saves detecting cost.

The invention provides a distiller's grain extract, a preparation method and application thereof. The extraction method is as follows: in a protective atmosphere, CdSe / ZnS quantum dots modified by trioctylphosphine, glyoxal dimethacrylate, 4-vinylpyridine and gallic acid were induced by azobisisobutyronitrile in two React in toluene, purify and dry to obtain the filler for extracting the antioxidant components of distiller's grains, and prepare it into a solid phase extraction column; add the distiller's grains to the ethanol-water mixture, heat and stir, and collect the extract; use the self-prepared solid phase extraction column Distiller's grain components are extracted, and distiller's grain extract is obtained after drying. The distiller's grain extract obtained by this method contains phenolic acids and can be used as a food additive to inhibit the production of carboxymethyllysine during food processing, expanding the new application of distiller's grains in liquor and providing a new source for resource utilization of distiller's grains way. The method for preparing distiller's grains extract provided by the invention has the advantages of simple and convenient extraction process, low cost, easy industrial application, and large amount of distiller's grains can be processed.

The invention discloses a method for detecting advanced glycation end products (AGEs) in main stream smoke of a cigarette. The method specifically comprises analysis and determination of carboxy methyl lysine (CML) and carboxyethyl lysine (CEL). The method comprises the following steps: trapping total particulate matter of cigarette smoke by virtue of a Cambridge filter; carrying out ultrasonic extraction on the filter with a hydrochloric acid solution; and carrying out analysis by combining a cation exchange solid-phase extraction column purification method with liquid chromatogram-tandem mass spectrometry (LC-MS / MS). The method has the characteristics that (1) a detection method for AGEs in the main stream smoke of the cigarette is built for the first time; (2) a sample pretreatment method is built according to the characteristics of cigarette smoke sample matrix, derivative reaction is not required, and the pretreatment is simple and efficient; and (3) the method is high in sensitivity, good in qualitative accuracy and suitable for analysis of large batches of samples.

The invention relates to a detection method and application of a carboxy methyl lysine ingredient in food. The detection method comprises the following steps of: carrying out derivatization by a derivating agent after protein acid in a sample is hydrolyzed; obtaining solution to be detected from the derived solution by solid-phase extraction; carrying out qualitative and quantitative analysis on the carboxy methyl lysine ingredient in the food by adopting a tandem mass spectrometry of isotope dilution ultra-performance liquid chromatography, wherein the derivating agent is 9-fluorenylmethyl chloroformate.

The invention relates to a method for detecting carboxy methyl lysine (CML) in soybean sauce. The method comprises the following steps: firstly, carrying out detection pretreatment on CML in a soybean sauce system, namely, detection pretreatment on free-state CML and combined-state CML; carrying out detection pretreatment on the free-state CML, namely sampling, adding a methanol solution to dilute, swirling and evenly mixing, purifying through a C18 solid-phase extraction column, adding a methanol solution to elute, filtering through a micro-filtration membrane to obtain a sample 1 to be detected; carrying out the detection pretreatment on the combined-state CML, namely sampling, reducing through sodium borohydride, separating polypeptide and protein through a dialysis bag, hydrolyzing through hydrochloric acid, disacidifying through vacuum, purifying through the C18 solid-phase extraction column, filtering through the micro-filtration membrane, drying in vacuum, re-dissolving to obtain a sample 2 to be detected; and subsequently, carrying out CML detection on the sample 1 and the sample 2 to be detected respectively. A total content of the CML is sum of the content of the free-state CML and the content of the combined-state CML. The sample pretreatment method has the characteristics of elimination of interference, protection of chromatographic column, safety and reliability, and capability of completely analyzing all the CML in the soybean sauce.