Method for measuring interaction efficiency of quinones substances and carboxymethyl lysine in solution

Abstract

The invention discloses a method for measuring the interaction efficiency of quinone substances and carboxymethyl lysine (CML) in a solution. The method comprises the following steps: respectively recording peak current and voltage characteristic values of the catechol substance solution before and after the CML is added by measuring a cyclic voltammetry curve of the catechol substance solution before and after the CML is added; and calculating the reduction rate of reduction peak current to obtain the interaction efficiency of the quinone substance in the solution and the CML. In the method,after the phenolic substance is oxidized to form the quinone substance, the quinone substance can rapidly interact with CML in a system, so that the side reaction of the quinone substance is reduced;the amount of the substance attached to the surface of an electrode is small, and the detection accuracy is high; the interference of factors such as the attachment of polyphenol and oxidation products thereof on a working electrode, the related side reaction of quinone substances and the like on the interaction of the quinone substances and CML in the prior art is reduced; and meanwhile, the method for measuring the interaction efficiency of quinone substances and carboxymethyl lysine in the solution is simple, short in analysis time, good in repeatability and convenience, low in use cost andwide in application.

Description

technical field [0001] The invention relates to the technical field of food safety detection and analytical chemistry, and more specifically relates to a method for measuring the interaction efficiency of quinones and carboxymethyllysine (CML) in a solution. Background technique [0002] CML (carboxymethyllysine) is the most widely studied type of typical advanced glycation end products (AGEs), which widely exists in various foods. After being digested and absorbed, CML can accumulate in tissues, and existing studies have proved that it is potentially harmful to human health. Therefore, research on the means of reducing CML content can effectively reduce its potential harm to human health. [0003] Catechols will be oxidized to form corresponding catechols while exerting anti-oxidation effect. As strong electrophiles, catechols are easy to undergo Michael addition reaction with amino groups on CML. So as to achieve the purpose of eliminating the system CML. At present, th...

AI Technical Summary

Problems solved by technology

Therefore, it is impossible to use existing means to measure the interaction between the quinones obtained from the oxidation of the above-mentioned polyphenols in the solution and the CML.

Images