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Method for detecting advanced glycation end-products in aquatic product

An end-product, middle-late technology, applied in the field of detection of advanced glycation end-products in aquatic products, can solve problems such as low efficiency, and achieve the effect of improving detection efficiency and shortening detection time.

Active Publication Date: 2020-04-10
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the prior art, the method for detecting AGEs by LC-MS / MS can only detect one or two AGEs components, and the efficiency is low

Method used

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  • Method for detecting advanced glycation end-products in aquatic product
  • Method for detecting advanced glycation end-products in aquatic product
  • Method for detecting advanced glycation end-products in aquatic product

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Experimental program
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Effect test

Embodiment

[0050] 1. Instruments and reagents

[0051] 6410Triple Quad Mass Spectrometer Agilent Technologies, USA

[0052] 1260Infinity Liquid Chromatograph Agilent Technologies, USA

[0053] Analytical balance Beijing Sartorius Scientific Instrument Co., Ltd.

[0054] XW-80A Micro Vortex Mixer Shanghai Huxi Analytical Instrument Factory Co., Ltd.

[0055] KQ-300VDE Dual Frequency CNC Ultrasonic Cleaner Kunshan Ultrasonic Instrument Co., Ltd.

[0056] N-20 multifunctional nitrogen blowing instrument Shandong Yunwang Data Technology Co., Ltd.

[0057] BCD-190TMPK Refrigerator Qingdao Haier Co., Ltd.

[0058] CML standard, CEL standard, CML-d 4 Standard, CEL-d 4 Standard Canada Toronto Research Chemicals

[0059]MG-H1 standard product American International Laboratory

[0060] MG-H1-d 3 Standard American BIOBERRY

[0061] Poly-Sery CNW MCX solid phase extraction column Shanghai Anpu Experimental Technology Co., Ltd.

[0062] LC-CQ-24F solid phase extraction device Shanghai Lich...

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Abstract

The invention provides a method for detecting advanced glycation end-products (AGEs) in an aquatic product by using liquid chromatography-tandem mass spectrometry. The method comprises the following steps of: 1, extracting AGEs; 2, making a standard curve: drawing the standard curve by taking the concentration value of a standard substance as the abscissa and taking the standard substance area*(internal standard substance concentration / internal standard substance area) as the ordinate to obtain an equation; and 3, subjecting the treated sample to HPLC-MS / MS detection in a detection mode of MRMto obtain the AGEs peak area in the sample, and acquiring the AGEs concentration in the sample. The invention aims to establish the liquid chromatography-tandem mass spectrometry method, which can detect three AGEs (carboxymethyl lysine (CML), carboxyethyl lysine (CEL) and methyl glyoxal hydroimidazolone MG-H1) at one time and has the advantages of good separation degree, good linear relationship, wide linear range and the like.

Description

technical field [0001] The invention belongs to the field of detection of food harmful substances, in particular to a method for detecting advanced glycation end products in aquatic products. Background technique [0002] Advanced Glycation End-products (Advanced Glycation End-products, AGEs) are produced under non-enzymatic conditions by a series of reactions between the free amino groups of macromolecular substances such as proteins, lipids or nucleic acids, and the aldehyde groups of reducing sugars. stable compound. Medical research results show that AGEs are closely related to the occurrence of many human diseases, and dietary intake is an important source of AGEs in the body. Due to the difficult handling of food samples and the variety and complex structure of AGEs, there is a lack of general and effective methods for the detection of AGEs in food. At present, the methods for measuring AGEs at home and abroad are divided into instrumental method and immunochemical m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/54G01N30/96G01N30/72G01N30/88
CPCG01N30/02G01N30/06G01N30/14G01N30/54G01N30/96G01N30/72G01N30/88G01N2030/027G01N2030/062G01N2030/146G01N2030/8813Y02A40/81
Inventor 董士远曾名湧安婧张琪赵元晖周鹏程
Owner OCEAN UNIV OF CHINA
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