Class-I carboxymethyl lysine removing agent and application thereof

A carboxymethyllysine and elimination agent technology, which is applied in the field of carboxymethyllysine elimination preparations, can solve the problems of inability to eliminate and completely inhibit AGEs, and achieve the reduction of CML levels, compensation for the ineffectiveness of AGEs, and good elimination The effect of action

Active Publication Date: 2016-09-07
广东中旭农业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In actual production, the food system often already contains a certain level of AGEs before the inhibitor is added, and the existing inhibitors cannot completely inhibit the production of AGEs in the system (the inhibition rate is ...

Method used

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  • Class-I carboxymethyl lysine removing agent and application thereof
  • Class-I carboxymethyl lysine removing agent and application thereof
  • Class-I carboxymethyl lysine removing agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Identification of 4-methyl-phthaloquinone (4MBQ) capture CML ability and identification of the structure of the additive product, specifically including the following steps:

[0073] (1) Prepare 4MBQ solution with 0.2M phosphate buffer (pH 4.5) so that its concentration is 0.08mM;

[0074] (2) Prepare a solution of 1-10 mM CML with 0.2 M phosphate buffer, so that after mixing with the above (1) solution in equal volume, the pH is 5.0, 7.0 or 8.0;

[0075] (3) Use Stopped-flow to measure the reaction kinetics of 4MBQ and CML by monitoring the absorbance change of the reaction system in the ultraviolet-visible region (200-750nm) at 25°C;

[0076] (4) Prepare a solution in which CML is 10 mM and 4MBQ is 1 mM with 0.2 M phosphate buffer (pH 7.0);

[0077] (5) Stir the mixed liquid described in (4) at room temperature for 1 min, and process it through a 0.22 μm microfiltration membrane to obtain the test solution;

[0078] Finally, LC-ESI-MS / MS was used to detect the prepa...

Embodiment 2

[0097] The structural identification of epicatechin (EC) quinones capturing CML addition products specifically includes the following steps:

[0098] (1) Use 0.2M phosphate buffer (pH 8.0) to prepare solutions with 0.1M lysine (Lys) and 0.1M glucose (Glu) respectively to simulate the food system;

[0099] (2) Take 1mL of the simulated food system described in (1), and add 2mM EC quinone;

[0100] (3) Heat the sample in a water bath at 80°C for 1 hour;

[0101] (4) Take 1 mL of the heated sample, add distilled water to make up to 5 mL;

[0102] (5) Take 1 mL of the diluted solution and pass it through a 0.22 μm microfiltration membrane, and use UPLC-ESI-MS / MS to carry out structural identification of the adduct.

[0103] Described UPLC adopts Agilent SB1290 model, and detection condition is:

[0104] Column: Agilent SB-C 18 Chromatographic column 2.1×50mm, 1.8μm

[0105] Column temperature: 25°C

[0106] Mobile phase: A) formic acid: water = 1:1000 (v:v), B) acetonitrile ...

Embodiment 3

[0119] The structural identification of epicatechin (EC) quinones capturing CML addition products specifically includes the following steps:

[0120] (1) Use 0.2M phosphate buffer (pH 8.0) to prepare solutions with 0.1M lysine (Lys) and 0.1M glucose (Glu) respectively to simulate the food system;

[0121] (2) Take 1mL of the simulated food system described in (1), and add 2mM EC quinone;

[0122] (3) Heat the sample in a water bath at 100°C for 1 hour;

[0123] (4) Take 1 mL of the heated sample, add distilled water to make up to 5 mL;

[0124] (5) Take 1 mL of the diluted solution and pass it through a 0.22 μm microfiltration membrane, and use UPLC-ESI-MS / MS to carry out structural identification of the adduct.

[0125] Described UPLC adopts Agilent SB1290 model, and detection condition is:

[0126] Column: Agilent SB-C 18 Chromatographic column 2.1×50mm, 1.8μm

[0127] Column temperature: 25°C

[0128] Mobile phase: A) formic acid: water = 1:1000 (v:v), B) acetonitrile...

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PUM

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Abstract

The invention discloses a class-I carboxymethyl lysine removing agent and application thereof. The removing agent is a quinones substance formed by the oxidization of polyphenol containing a catechol or pyrogallol structure unit. The removing agent is applied to the reduction of the content of carboxymethyl lysine in a food system. The quinones substance has stronger capability of removing AGEs (Advanced Glycation End Products) in the food system of which the pH is neutral or alkaline, and when the quinones substance is added after the food system generates the CML or before the CML is generated, the level of the CML in the system can be effectively reduced.

Description

technical field [0001] The invention relates to the field of food processing, in particular to a class of carboxymethyllysine (CML) elimination preparations, which can reduce the content of CML produced in food systems by capturing CML, and are widely used as food additives. Background technique [0002] Advanced glycation end products (AGEs) are a class of highly oxidized compounds produced by foods or organisms in the late stage of the Maillard reaction. Existing studies have shown that AGEs may be related to the occurrence and development of diseases such as diabetes, chronic heart failure, and atherosclerosis. Food-derived AGEs are absorbed into the human body through the digestive tract, which will increase the level of AGEs in the human body. Long-term intake of excessive food-derived AGEs, especially free AGEs (glycosylated amino acids), will cause potential health hazards to the human body. [0003] Existing means of reducing AGEs content mainly is to suppress the ...

Claims

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Application Information

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IPC IPC(8): A23L5/20A23C7/04
CPCA23C7/043A23V2002/00A23V2250/2132A23V2250/21166A23V2250/21A23V2250/21168A23V2250/2117A23V2250/2116
Inventor 李琳李玉婷李冰梁毅张霞梁志理苏健裕徐振波
Owner 广东中旭农业股份有限公司
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