A method for rapid determination of hyaluronic acid content in fermented broth
A hyaluronic acid, rapid determination technology, applied in the direction of measuring device, color/spectral characteristic measurement, material analysis by optical means, etc., to achieve the effect of overcoming band overlapping, simple and easy method, and strengthening band characteristics
Active Publication Date: 2011-12-28
BLOOMAGE BIOTECHNOLOGY CORP LTD
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[0004] In order to solve the above-mentioned prior art methods for measuring the content of hyaluronic acid in the fermented liquid consume reagents, complex operation, fermentation substrates seriously interfere with the measurement results, etc., the present invention Provided is a method capable of rapidly determining the content of hyaluronic acid in a fermented liquid. The method of the present invention is green, pollution-free, simple and easy to implement, and can quickly and reliably measure the content of hyaluronic acid in a fermented liquid
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Embodiment 1
[0029] (1) First, take 7 batches of hyaluronic acid fermentation broth with the same fermentation conditions. The fermentation broth contains 2wt% peptone, 1.5wt% yeast powder, 6wt% glucose, 0.02wt% dipotassium hydrogen phosphate, and 0.1wt% magnesium sulfate %, sodium glutamate 0.1wt%, fermented in a 5-ton fermenter under neutral conditions at 37°C, and the fermentation period was about 24 hours;
[0030] (2) According to the hyaluronic acid fermentation cycle, the fermentation broth was collected every 2 hours starting from the 10th hour. Under the condition of 4°C, the original near-infrared spectrum of the above fermentation broth was measured by the Antaris II near-infrared spectrometer of Thermo Fisher Company. Spectrum, such as figure 1 shown, and adopt the carbazole colorimetric method to measure the actual content of hyaluronic acid wherein, see Table 1 for details,
[0031] Table 1 Hyaluronic acid content in fermentation broth
[0032]
[0033] (3) In order to...
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The invention relates to a method for rapidly determining the content of hyaluronic acid in fermentation liquor by using near infrared spectroscopy in the technical field of determining the hyaluronic acid content in hyaluronic acid. The method comprises the following steps: selecting the hyaluronic acid fermentation liquor with same fermentation condition; determining the practical content of hyaluronic acid at regular intervals, determining the original near infrared spectroscopy of different batches of fermentation liquor by using a near infrared spectroscopy analyzer under the same temperature condition; pretreating the original near infrared spectroscopy by using a first order derivative+Norris5 site smooth method, in a wave band scope of 7312.7cm<-1>-10000cm<-1>, associating the practical content of hyaluronic acid in fermentation liquor with pretreated near infrared spectroscopy and establishing a mathematics model by using a partial least square method; taking the fermentationliquor with the hyaluronic acid content to be detected, determining the spectra of the near infrared spectroscopy and predicting the hyaluronic acid content according to the mathematics model. The method of the invention has the advantages of environmental protection without pollution, simple operation, and enables a rapid and reliable determination of the content of hyaluronic acid in fermentation liquor; the error is small.
Description
technical field [0001] The invention relates to the technical field of hyaluronic acid content determination in fermented liquid, in particular to a method for rapidly measuring hyaluronic acid content in fermented liquid by using near-infrared spectroscopy. Background technique [0002] Hyaluronic acid (HA), also known as hyaluronic acid, is an acidic mucopolysaccharide with unique viscoelasticity and physiological functions. It is the main component of extracellular matrix and is widely used in industries such as medicine, beauty and health food . [0003] Content is an important parameter in the fermentation process of hyaluronic acid, which has important guiding significance for the selection of fermentation strains, process optimization and fermentation process control. At present, the content determination method of hyaluronic acid is mainly through color development after degradation, and the content of glucuronic acid or glucosamine in it is measured according to ...
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IPC IPC(8): G01N21/35G01N21/3577G01N21/359
Inventor 臧恒昌董芹刘爱华郭学平栾贻宏石艳丽张惠
Owner BLOOMAGE BIOTECHNOLOGY CORP LTD
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