Method for measuring alkaloid in tobacco and tobacco product

Abstract

The invention relates to a method for measuring alkaloid in tobacco and tobacco products, which is characterized by dissociating alkaloid from the tobacco and tobacco products by using sodium hydroxide solution, extracting the alkaloid from samples by using triethylamine/trichloromethane solution, and detecting content of five kinds of alkaloid with GC-MS ( Gas Chromatograph-Mass Spectrum) quantitative analysis. Compared with other methods for analyzing the alkaloid, the invention has the advantages that alkaloid is separated from the tobacco by DB-35MS chromatographic columns so as to separate nornicotine and myosmine in the tobacco and accurately quantify target matters; batch sampling is carried out on the separation of nicotinamide and other alkaloid so as to detect the nicotinamide with high content in the tobacco and meet the quantitative analysis of other alkaloid with low content; the standard working curves of standard matters are separately compounded so as to avoid influence on quantization of other alkaloid by alkaloid catabolite existing in the standard samples. The method is simple and convenient and has the advantages of good repeatability, high analyzing and measuring sensitivity, and accurate quantization.

Description

technical field [0001] The invention belongs to a method for measuring chemical components of tobacco, in particular to a method for measuring alkaloids in tobacco and tobacco products. Background technique [0002] Alkaloids are important components that affect the quality of tobacco leaves. In addition to nicotine, the main alkaloids in tobacco include nornicotine, anatine, mesmin and pseudobasine. Nicotine can directly stimulate the central nervous system of the human body and produce physiological reactions; nornicotine has a negative impact on the taste of cigarettes; mesmin has a mouse smell, which seriously affects the taste of cigarette products; alkali. Alkaloids are one of the direct precursors of tobacco-specific nitrosamines, which are easily metabolized into alkaloids in the process of tobacco preparation and cigarette smoking, and then form carcinogenic tobacco-specific nitrosamines with nitrite, especially The conversion of nicotine to nornicotine is one of ...

AI Technical Summary

Problems solved by technology

Previous literature surveys have shown that there are three problems in the analysis of the main alkaloids in tobacco: 1) The content of nicotine in tobacco is quite different from other alkaloids, and the content of nicotine is usually tens of milligrams per gram. It is a few micrograms per gram to tens of micrograms per gram. When nicotine and mesmin are detected simultaneously, the injection volume is too small, which has a great impact on the detection of mesmin. If the injection volume is too large, the nicotine The detection amount exceeds the corresponding range of the detector; 2) Nornicotine and mesmine in tobacco cannot be separated well. The literature usually uses a DB-5ms capillary column. Due to the peak tailing of nornicotine, It affects the separation and quantification of mesmin, which is very close to its peak; 3) In the quantification of tobacco alkaloids, the solution configuration of the marking curve is defective

Since nicotine is easily converted into nornicotine, mesmin, pseudobasine and other compounds, nornicotine is also easily converted into mesmin, so in the configuration of the alkaloid calibration curve, the nicotine to be analyzed should be , nornicotine and other alkaloid standard solutions are configured separately, if the mixed configuration will affect the accurate quantification of nornicotine and other alkaloids, but the solution configuration of the calibration curve in the literature is the configuration of the standard sample mixed solution