Dry chemical quantitative test strip for removing interference, gluten or aspartate aminotransferase quantitative test strip and detection method

An aspartate aminotransferase and dry chemical technology, which is applied in the field of dry chemical test strips and detection fields of aminotransferase, can solve problems such as easy occurrence of false positive results and the like

Active Publication Date: 2011-12-21
SINOCARE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using the rate method (that is, testing the change rate of color development), removing endogenous interference requires more complicated optical instruments, often using optical devices such as integrating spheres, and the normal value of transaminase is generally very low, and alanine aminotransferase is 5U/L ~40U/L, aspartate aminotransferase is 8

Method used

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  • Dry chemical quantitative test strip for removing interference, gluten or aspartate aminotransferase quantitative test strip and detection method
  • Dry chemical quantitative test strip for removing interference, gluten or aspartate aminotransferase quantitative test strip and detection method
  • Dry chemical quantitative test strip for removing interference, gluten or aspartate aminotransferase quantitative test strip and detection method

Examples

Experimental program
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Embodiment 1

[0056] a kind of like figure 2 The shown dry chemical quantitative test strip for removing interference, the test strip includes a substrate 7, on which a sample application area 13 is fixed with an interference-removing reagent and a signal-providing reagent (in this embodiment, specifically includes a diffusion Layer 1, sample pad 2, blood filter membrane 3, enzyme binding pad 4 and flow pad 5), the sample loading area 13 is directly fixed on the substrate 7; the substrate 7 is also provided with a starting area fixed with a test reaction initiation reagent 14. The activation area 14 includes a reagent layer 6, the reagent layer 6 is connected to one end of the substrate 7 through an adhesive 8, and the adhesive 8 has a sufficient height to allow most of the reagent layer 6 to be suspended in the sample loading area. Zone 13 above. The sample application area 13 and the activation area 14 remain non-contact under normal conditions, and the activation area 14 (reagent layer...

Embodiment 2

[0060] a kind of like image 3 The shown dry chemical quantitative test strip for removing interference, the test strip includes a substrate 7, on which a sample application area 13 is fixed with an interference-removing reagent and a signal-providing reagent (in this embodiment, specifically includes a diffusion Layer 1, sample pad 2, blood filter membrane 3, enzyme binding pad 4 and flow pad 5), the sample loading area 13 is directly fixed on the substrate 7; the substrate 7 is also provided with a starting area fixed with a test reaction initiation reagent 14. The activation area 14 includes a reagent layer 6, the reagent layer 6 is connected to one end of the substrate 7 through an adhesive 8, and the adhesive 8 has a sufficient height to allow most of the reagent layer 6 to be suspended in the sample loading area. Zone 13 above. The sample application area 13 and the activation area 14 remain non-contact under normal conditions, and the activation area 14 (reagent layer ...

Embodiment 3

[0064] a kind of like Figure 4 Compared with the test strip provided in Example 1, the shown dry chemical quantitative test strip for removing interference does not have an enzyme binding pad 4, and the rest of the structure and application methods are the same as in Example 1 . Since the enzyme-binding pad 4 is not provided, substances originally immobilized on the enzyme-binding pad 4 (such as various catalytic reaction enzymes) can be directly immobilized on other structures in the sample loading area 13 (such as the flow pad 5 ).

[0065] The test strips of the above several embodiments, when cut into strips, may make the activation area contact with the sample application area, and the reagents fixed in the activation area to initiate the reaction may partially adhere to the sample application area. On the flow pad, the initiation reaction is uncontrollably advanced. This kind of problem can be solved by various methods, for example, an interlayer structure is provided...

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Abstract

The invention discloses an interference-removing dry chemical quantitative test strip. The strip comprises a bottom liner, wherein the bottom liner is provided with a sample addition region and also a starting region; the sample addition region is fixed with interference-removing reagents and signal providing reagents; the starting region is fixed with test reaction starting reagents; and the sample addition region and the starting region are arranged on the test strip in a mode of maintaining a non-contact under a normal state and mutually contacting under a test state. The test method comprises the following steps of: adding a sample solution to be tested into the sample addition region, re-dissolving materials fixed on the sample addition region into the sample solution to be tested, and starting to perform a signal reaction to obtain a background signal value; and then enabling the starting region to contact with the sample addition region, re-dissolving materials fixed on the starting region, starting a test reaction, performing a second-time signal reaction on a product again, and estimating out a content of a biological enzyme to be tested according to a tested second-time characteristic value and the background signal value. The test method provided by the invention has the advantages of convenience for operation, low test cost, accurate test result and high test precision and can specifically be used in the alanine and aspartate aminotransferase quantitative test.

Description

technical field [0001] The invention relates to a dry chemical test strip and a test method, in particular to a dry chemical test strip and a detection method for transaminase. Background technique [0002] There are many interferences in dry chemical tests, such as the interference of uric acid and ascorbic acid in the determination of redox reaction, the interference of pyruvate in the detection of transaminase, especially the interference of pyruvate in transaminase is more serious. [0003] There are many kinds of transaminases, among which alanine aminotransferase (GPT / ALT) and aspartate aminotransferase (GOT / AST) are the most important. Alanine aminotransferase, also known as alanine aminotransferase, catalyzes the transamination between glutamic acid and pyruvate; of transamination. Clinically, the determination of these two transaminases in whole blood, serum, plasma, and interstitial fluid is a very important indicator in the diagnosis of heart disease, muscle dis...

Claims

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Application Information

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IPC IPC(8): G01N33/52G01N33/558G01N21/31G01N21/76G01N21/64
Inventor 吉翔谢光李宗祥车宏莉
Owner SINOCARE
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