Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Sequencing of nucleic acids

A nucleic acid and nucleic acid base sequence technology, applied in the field of high-throughput parallel DNA sequencing, can solve the problem of large differences in the copy number of captured sequences, and achieve the effects of reducing sequencing redundancy requirements, reducing sequencing time, and high capacity

Active Publication Date: 2010-12-15
杭州联川基因诊断技术有限公司
View PDF12 Cites 25 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The capture nature of this probe is essentially random and the copy number of the captured sequence varies widely

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sequencing of nucleic acids
  • Sequencing of nucleic acids
  • Sequencing of nucleic acids

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0058] The methods of the invention are not limited to the types of molecules already discussed. In a preferred embodiment of the present invention, DNA, RNA, polypeptides, carbohydrates or other molecules can be synthesized in situ on addressable nanobeads in an amendable manner. At the same time, the synthesis method of the present invention is not limited to the number of reaction chambers that can be used to synthesize molecular nanobeads. Such as Figure 5 As shown, when a single reaction chamber is used, it is conceivable that each monomer species is added to multiple reaction chambers. Reaction chambers can also be utilized simultaneously far beyond a single step. Additional synthetic steps include the utilization of dimers and trimers or longer available elements.

[0059] The number of different elements to be added will determine the minimum number of reaction chambers necessary, such that one reaction chamber is necessary for each element. For example, using nat...

Embodiment 1

[0119] Example 1 Sequence in Elution Capillary Bundle

[0120] The sequencing CE block is made of drawn glass, and a capillary with an inner diameter of 100 m is used to form a depressed channel bundle with a channel cross-sectional area of ​​2x3 mm2 and a length of 5 cm. Fill the sequencing channel with 10% PAGE gel by capillary effect, and allow the solution to flow through half of the bottom area (perpendicular to the channel) to load the sample (see below). The samples used contained oligonucleotides of different lengths labeled with four fluorescent dyes. These 4 oligonucleotides are FAM-18mer, Cy3-6mer, Cy3-38mer and FAM-46mer. Afterwards, place the sequencing CE block in the horizontal electrophoresis device for a certain period of time (minutes), take it out and use a fluorescence microscope (Olympus BX41EPI fluorescence research microscope) to obtain images of the outer end surface, and then put it back into the electrophoresis device to continue. Repeating this pro...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
heightaaaaaaaaaa
lengthaaaaaaaaaa
Login to View More

Abstract

The present invention relates to the field of analysis of nucleic acid sequences. More specifically, the present invention relates to the method and instrument for high throughput parallel DNA sequencing. The present invention also provides method for selection of sequences from analyte samples for enrichment of the target sequences or depletion of the selected molecules and in particular undesirable sequence templates from sequencing samples.

Description

[0001] Refer to related applications [0002] This application claims priority from US Provisional Application No. 61 / 012,468, filed October 10, 2007. Its text is incorporated herein by reference. field of invention [0003] The present invention relates to the field of nucleic acid sequence analysis. More specifically, the present invention relates to methods and devices for high-throughput parallel DNA sequencing. The present invention also provides a method for screening sequences in samples for enriching target sequences or removing specific molecules, especially unnecessary sequence templates in sequencing samples. Background technique [0004] The DNA genome provides the code for fundamental biological systems, and the RNA transcriptome provides the footprint of proteins and other functionally scientifically valuable RNA elements. The field of DNA / RNA sequencing is critical to deciphering these systems and thus has experienced exponential growth over the past few de...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q2565/50C12Q2565/518
Inventor 高晓莲周小川
Owner 杭州联川基因诊断技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com