Detection method of cassia twig, peony and anemarrhena decoction

A detection method, peony technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem that cinnamic acid cannot be detected, cannot effectively reflect the quality of Guizhi Shaoyao Zhimu Decoction, and cannot effectively reflect the quality of Guizhi Shaoyao Zhimu Decoction Effective control and other issues to achieve the effect of broad application prospects

Active Publication Date: 2019-05-31
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE +1
View PDF3 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the research on Guizhi Shaoyao Zhimu Decoction mainly focuses on its pharmacodynamics research, and there are few reports on its material basis research, such as Dong Yunzhuo. HPLC simultaneously determines the content of 7 active ingredients in Guizhi Shaoyao Zhimu Decoction, Onl

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method of cassia twig, peony and anemarrhena decoction
  • Detection method of cassia twig, peony and anemarrhena decoction
  • Detection method of cassia twig, peony and anemarrhena decoction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The characteristic spectrum of embodiment 1 Guizhi Shaoyao Zhimu Decoction

[0029] 1) Preparation of reference solution: take gallic acid, mangiferin, paeoniflorin, acteoside, liquiritin, 5-O-methylvisamidol glycoside, cinnamic acid and ammonium glycyrrhizinate reference substances respectively, and accurately Weigh and add methanol to make 2.342, 1.048, 0.561, 1.857, 0.945, 1.086, 0.503, 1.114g L -1 Reference substance stock solution. Accurately draw the appropriate amount of the above 8 reference substance stock solutions, mix them, and add methanol to make the concentrations respectively 0.1171, 0.1048, 0.0561, 0.1857, 0.0945, 0.1086, 0.0503, 0.1114g L -1 mixed reference solution.

[0030] 2) Preparation of the test solution:

[0031] Guizhi Shaoyao Zhimu Decoction Material Standard Freeze-dried Powder: According to the original prescription of Guizhi Shaoyao Zhimu Decoction in "Synopsis of the Golden Chamber" (namely: four liang of Guizhi, three liang of peony, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a detection method of cassia twig, peony and anemarrhena decoction, where detecting is achieved by means of high-performance liquid chromatography. The detection method includesthe steps of 1) preparing reference solutions, to be specific, collecting controls of gallic acid, mangiferin, paeoniflorin, prim-o-glucosylcimifugin, liquiritin, 5-O-methylvisammioside, cinnamic acid and ammonium glycyrrhetate, and adding methanol solution respectively to obtain the reference solutions; 2) preparing a test solution, to be specific, adding methanol to dissolve lyophilized powderof cassia twig, peony and anemarrhena decoction to obtain the test solution; 3) sucking the reference solutions and the test solution, and injecting them into a liquid chromatograph. The detection method is suitable for detecting gallic acid, mangiferin, paeoniflorin, prim-o-glucosylcimifugin, liquiritin, 5-O-methylvisammioside, cinnamic acid and ammonium glycyrrhetate, allows overall quality of the cassia twig, peony and anemarrhena decoction to be controlled, also helps provide methodological basis for the study on the material basis of the cassia twig, peony and anemarrhena decoction, and is worthy of popularization and application.

Description

technical field [0001] The invention specifically relates to a detection method of Guizhi Shaoyao Zhimu Decoction. Background technique [0002] Guizhi Shaoyao Zhimu Decoction comes from "Synopsis of the Golden Chamber · Stroke, Lijie, Syndrome, Syndrome and Treatment". It has the functions of expelling wind and dehumidification, warming meridians and dispelling cold, nourishing yin and clearing heat. Rheumatoid arthritis (RA) belongs to the category of arthralgia in traditional Chinese medicine. Modern clinical and pharmacological studies have found that Guizhi Shaoyao Zhimu Decoction has definite curative effect on RA. The pathogenesis of RA is not yet clear, but studies have shown that the level of free radicals in the serum and joint fluid of RA patients and model animals increases, and free radicals play an important role in the damage of articular cartilage in RA diseases. Guizhi Shaoyao Zhimu Decoction can Effectively scavenge free radicals. [0003] At present, th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02
Inventor 黄勤挽高永翔谭瑾覃艺耿福能
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap