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Establishment method for HPLC (high performance liquid chromatography) fingerprint of cold treatment medicament

A technology for establishing methods and fingerprints, applied in measuring devices, instruments, scientific instruments, etc., can solve the problem that the content of effective active ingredients cannot represent the curative effect, and achieve the effects of easy realization of chromatographic conditions, good precision, and simple production

Active Publication Date: 2016-09-07
SHANGHAI PHARMA GRP QINGDAO GROWFUL PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For traditional Chinese medicine, the content of any single active ingredient cannot represent the overall curative effect

Method used

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  • Establishment method for HPLC (high performance liquid chromatography) fingerprint of cold treatment medicament
  • Establishment method for HPLC (high performance liquid chromatography) fingerprint of cold treatment medicament
  • Establishment method for HPLC (high performance liquid chromatography) fingerprint of cold treatment medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1 Instruments and reagents

[0044] 1.1 Instrument

[0045] Waters 2695 high performance liquid chromatography, Waters 2998 detector, Waters Empower chemical workstation; Traditional Chinese medicine chromatographic fingerprint similarity evaluation system (version 2004A); BUG25-12 ultrasonic cleaning machine (220W, 50KHZ, Shanghai Branson Ultrasonic Co., Ltd.); AEG-45SM electronic balance (1 / 100,000, Mettler, Switzerland), BP121S (1 / 10,000, Beijing Sartorius Scientific Instrument Co., Ltd.).

[0046] 1.2 Reagent

[0047] All reagents are analytically pure except that the mobile phase is chromatographically pure; glycyrrhizic acid reference substance (Beijing Hengyuan Qitian Chemical Technology Research Institute, batch number MUST-13083101); liquiritin reference substance (Beijing Hengyuan Qitian Chemical Technology Research Institute) Institute, batch number MUST-130020901); chlorogenic acid reference substance (Beijing Hengyuan Qitian Chemical Technology Research I...

Embodiment 2

[0090] A method for establishing the HPLC fingerprint of a cold medicine, the steps of which are the same as in Example 1.

[0091]The raw materials of cold medicine are: ephedra 6g, mint 5g, cicada slough 5g, honeysuckle 15g, scutellaria baicalensis 12g, bitter almond 9g, Fritillaria 6g, bellflower 6g, licorice 5g; preparation method: mint steam distillation extracts volatile oil, distilled The aqueous solution is stored in another container; the dregs and the remaining ephedra and other eight flavors are decocted with water for 1-3 times, each time for 0.5-1.5 hours, adding 6-10 times the weight of water for the first time, adding 5-8 times the weight of water for the second or third time, Combine the decoction, filter, combine the filtrate with the above aqueous solution, and concentrate to a clear paste with a relative density of 1.18 to 1.20 at a relative density of 90 to 95°C; take the clear paste, add 1-3 parts by weight of sucrose, and 1-3 parts by weight of dextrin, T...

Embodiment 3

[0093] A method for establishing the HPLC fingerprint of a cold medicine, the steps of which are the same as in Example 1.

[0094] The raw materials of cold medicine are: ephedra 3g, mint 9g, cicada slough 3g, honeysuckle 17g, scutellaria baicalensis 9g, bitter almond 16g, Fritillaria 3g, bellflower 8g, licorice 4g; The aqueous solution is stored in another container; the dregs and the remaining ephedra and other eight flavors are decocted with water for 1-3 times, each time for 0.5-1.5 hours, adding 6-10 times the weight of water for the first time, adding 5-8 times the weight of water for the second or third time, Combine the decoction, filter, combine the filtrate with the above aqueous solution, concentrate to a clear paste with a relative density of 1.18-1.20 at a relative density of 90-95°C; the obtained volatile oil is encapsulated with an appropriate amount of β-cyclodextrin, and an appropriate amount of clear paste is added for coloring, and sprayed Drying; take the ...

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Abstract

The invention discloses an establishment method for an HPLC (high performance liquid chromatography) fingerprint of a cold treatment medicament. The method comprises the following steps: (1) preparing reference solvents: preparing glycyrrhizic acid, liquiritin, chlorogenic acid and cynaroside reference solvents respectively; (2) preparing a test solvent: weighing bitter sweet granules, performing extraction, and filtering an extracting solvent with a microfiltration membrane to obtain the test solvent; (3) performing measurement to obtain the fingerprint by adopting an HPLC method, wherein a chromatographic condition is gradient elution, a flowing phase A of gradient elution is acetonitrile, B is a 0.05 to 5 percent formic acid aqueous solvent, and the detection wavelength is 254nm; (4) evaluating the similarity: evaluating the fingerprint of a test sample by adopting the Similarity Evaluation System for Chromatographic Fingerprint of TCM (Version 2004 A). the method is high in precision, repeatability and stability, and the quality of the bitter sweet granules can be effectively controlled by adopting an HPLC fingerprint technology.

Description

technical field [0001] The invention relates to a quality control method of a medicine for treating colds, in particular to a method for establishing an HPLC fingerprint of a medicine for treating colds. Background technique [0002] Chinese patent medicine for cold medicine is a complex system composed of multiple components, multiple factors, and multiple targets. The diversity and complexity of its chemical components are the material basis for its curative effect. The problem of analysis and evaluation, improving the existing quality control methods of traditional Chinese medicine is the focus and difficulty of attention in recent years. [0003] At present, most of the quality testing methods of traditional Chinese medicine still use spectral or chromatographic methods to identify or measure one or several active ingredients or index ingredients, as well as the routine inspection items stipulated in the Pharmacopoeia. For traditional Chinese medicine, the content of an...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06G01N30/02
CPCG01N30/02G01N30/06G01N30/88G01N2030/027G01N2030/062G01N2030/8809
Inventor 崔清华姜作玲田景振侯林洪毅高莉
Owner SHANGHAI PHARMA GRP QINGDAO GROWFUL PHARMA CO LTD
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