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Method for separating and preparing liquiritin

A technology of liquiritin and grassland is applied in the field of separation and preparation of liquiritin, which can solve the problems of inability to large-scale production, low product purity, poor reproducibility, etc., and is suitable for industrialized large-scale production, high sample purity, and short cycle. Effect

Active Publication Date: 2008-10-22
浙江华谱新创科技有限公司
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  • Summary
  • Abstract
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Problems solved by technology

The literature reports on the extraction, separation and preparation of liquiritin basically adopt traditional methods such as solvent extraction and silica gel chromatography (Zhu Xumin et al. Chinese Herbal Medicine, 2003, 34(3): 199-201; Liu Qin et al. Acta Pharmaceutica Sinica. 1989, 24(7): 525-531; Nakanishi T et al.Phytochemistry, 1985, 24(2): 339), these methods have low product purity, poor reproducibility, long time-consuming, serious organic residues, and cannot be produced on a large scale and other shortcomings

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  • Method for separating and preparing liquiritin
  • Method for separating and preparing liquiritin

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Crush the licorice grass medicinal material, weigh 1 kg quantitatively, put it in a 50-liter extraction tank, add 10 liters of water to decoct for 2 hours, filter, and save the filtrate for later use, add 10 liters of water to the filter residue and decoct for 2 hours, filter, and the filtrate and the second Once combined, the filter residue was discarded. The extract was concentrated by rotary evaporation to obtain 240 ml of extract with a relative density of 1.12. Add 412 milliliters of 95% ethanol to the extract, stir well, leave it in a refrigerator at 0°C for 24 hours, filter, discard the filter residue, and concentrate the filtrate to 160 milliliters, relative density 1.05, add 854 milliliters of 95% ethanol to the concentrated solution, fully Stir, refrigerate and stand at 0°C for 24 hours, filter, discard the filter residue, concentrate the filtrate until it has no alcohol smell, and centrifuge the concentrated solution in a high-speed centrifuge (20,000 rpm) to...

Embodiment 2

[0029]Crush the licorice grass medicinal material, weigh 0.5 kg quantitatively, put it in a 20-liter extraction tank, add 6 liters of water to decoct for 1 hour, filter, save the filtrate for later use, add 5 liters of water to the filter residue and decoct for 3 hours, filter, and the filtrate and the second Once combined, the filter residue was discarded. The extract was concentrated by rotary evaporation to obtain 140 ml of extract with a relative density of 1.10. Add 240 milliliters of 95% ethanol to the extract, fully stir, refrigerate and stand for 24 hours at 0°C, filter, discard the filter residue, and concentrate the filtrate to 40 milliliters, relative density 1.07, add 428 milliliters of 95% ethanol to the concentrated solution, fully Stir, refrigerate and stand at 0°C for 24 hours, filter, discard the filter residue, concentrate the filtrate until it has no alcohol smell, and centrifuge the concentrated solution in a high-speed centrifuge (20,000 rpm) to obtain 140...

Embodiment 3

[0034] Crush the licorice grass medicinal material, weigh 5 kg quantitatively, put it in a 250-liter extraction tank, add 60 liters of water to decoct for 2 hours, filter, save the filtrate for later use, add 60 liters of water to the filter residue and decoct for 2 hours, filter, and the filtrate and the second Once combined, the filter residue was discarded. The extract was concentrated by rotary evaporation to obtain 1.2 liters of extract with a relative density of 1.13. Add 2.1 liters of 95% ethanol to the extract, stir fully, and refrigerate at 0°C for 24 hours, filter, discard the filter residue, and concentrate the filtrate to 0.7 liters, relative density 1.08, add 3.8 liters of 95% ethanol to the concentrated solution, fully Stir, refrigerate and stand at 0°C for 24 hours, filter, discard the filter residue, concentrate the filtrate until it has no alcohol smell, and centrifuge the concentrated solution in a high-speed centrifuge (20,000 rpm) to obtain 1.5 liters of gl...

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Abstract

The invention relates to the separation of natural medicines, in particular to a separation and preparation method of liquiritin; wherein, extracting solution is obtained by decocting the ground licorice plants with ten times of water, and then extract is obtained after the extracting solution is concentrated, and then ethanol is added for alcohol precipitation twice, and then a 6000Da molecular weight membrane separator is used for separating the extract, and then membrane separation permeation liquid is separated by X-5 macro-porous absorption resin and is eluted with the ethanol water, and the obtained macro-porous resin separation components are separated by an efficient industrial chromatographic column and eluted with gradients with the methanol water and then the liquiritin is obtained by collecting, concentrating, freezing and drying the eluting solution. The method is high in product purity, great in preparation amount and stable and reliable in process and especially suitable for separating and preparing great amount of highly purified liquiritin compounds from the traditional Chinese medicine licorice.

Description

technical field [0001] The invention relates to the separation of natural medicines, in particular to a method for the separation and preparation of liquiritin, which is a preparation method for separating liquiritin from traditional Chinese medicine licorice through industrial chromatographic technology. Background technique [0002] Licorice is the roots and rhizomes of Glycyrrhiza Linn. plants of the Leguminosae family Glycyrrhiza Ural, Glycyrrhiza Glabra, and Glycyrrhiza inflate. It is distributed all over the world, mostly in Eurasia. my country is mainly distributed in the provinces and regions north of the Yellow River Basin, and individual species are found in the northwestern part of Yunnan. Licorice roots are flat in nature and sweet in taste, and have the functions of nourishing the spleen, nourishing the lungs, detoxifying, and harmonizing various medicines. In recent years, many new pharmacological effects have been discovered through research. Licorice has bio...

Claims

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Application Information

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IPC IPC(8): C07H15/26A61K36/484
Inventor 梁鑫淼张慧丰加涛李伟徐青
Owner 浙江华谱新创科技有限公司
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