Method for simultaneous determination of six active components in Niuhuang Ninggong tablet

A technology of active ingredients and determination methods, applied in the field of medicine, can solve problems such as difficult to truly control the quality of drugs, illegal production, and incomplete control, and achieve the effects of good reproducibility and stability, high separation, and simple methods
CN104897787AInactive Publication Date: 2015-09-09JILIN NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JILIN NORMAL UNIV
Publication Date
2015-09-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for simultaneous determination of six active components consisting of chrysophanol, emodin, liquiritin, forsythin, baicalin and berberine hydrochloride in a Niuhuang Ninggong tablet through HPLC. Chromatographic conditions employed in the invention are as follows: a chromatographic column is TC-C18 (4.6 mm * 250 mm, 5 [mu]m); detection wavelength is 280 nm; a mobile phase is methanol-0.05% phosphoric acid; gradient elution comprises three parts, i.e., elution with methanol with a concentration varying in a range of 10 to 80% in the time period from 0 min to 35 min, then elution with methanol with a concentration of 80% in the time period from 35 to 50 min, and finally elution with methanol with a concentration varying in a range of 80 to 10% in the time period from 50 to 60 min; flow velocity is 1.0 mL / min; column temperature is 25 DEG C; and sample size is 10 [mu]L. Under the above-mentioned chromatographic conditions, chromatographic peaks are perfectly separated, and concentrations and peak areas of chrysophanol, emodin, liquiritin, forsythin, baicalin and berberine hydrochloride show good linear relation. The method is simple, rapid and accurate, has good repeatability and can provide quality bases for comprehensive evaluation and control of the Niuhuang Ninggong tablet.
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Description

technical field

[0001] The invention relates to a method for detecting active components in the Chinese patent medicine Niuhuang Ninggong Tablet, specifically a method for simultaneously measuring six active components in the Niuhuang Ninggong Tablet, which belongs to the technical field of medicine. Background technique

[0002] Niuhuang Ninggong Tablets are used for exogenous febrile diseases, high fever, coma, convulsions, dizziness due to liver yang, tinnitus, headache, restlessness, insomnia, and epilepsy. It has a certain anti-relapse effect on schizophrenia.

[0003] Niuhuang Ninggong Tablets is a medicine made by extracting and processing 27 herbs such as bezoar, dandelion, realgar, forsythia, rhubarb, borneol, honeysuckle, licorice, coptis, scutellaria, uncaria, rhubarb magnetite (calcined), scrophulariaceae, and kudzu root. . The preparation is included in WS3-B-2852-98 on the 36th page of the 15th volume of the Chinese Ministry of Health's drug standard tradition...

Claims

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