Method for simultaneous determination of six active components in Niuhuang Ninggong tablet
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of active ingredients and determination methods, applied in the field of medicine, can solve problems such as difficult to truly control the quality of drugs, illegal production, and incomplete control, and achieve the effects of good reproducibility and stability, high separation, and simple methods
Inactive Publication Date: 2015-09-09
JILIN NORMAL UNIV
View PDF4 Cites 9 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
At present, it is difficult to truly control the quality of medicines due to the control of single active ingredients or index ingredients in Chinese patent medicines
In order to reduce costs, some pharmaceutical factories make use of the loopholes in drug inspection quality standards that cannot completely control the taste of all medicines, and illegally produce
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0030] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments, so that those skilled in the art can better understand the present invention, but the present invention is not limited thereto.
[0031] Instruments and reagents
[0032] Instruments: Agilent 1100 high performance liquid chromatograph in the United States; Shimadzu UV spectrophotometer UV2550 in Japan; GWA-UN4 series ultrapure water device (Beijing General Instrument Co., Ltd.); FA1104N 1 / 10,000 analytical balance (Shanghai Precision Science Instrument Co., Ltd. Tianping Instrument Factory); SK5200H ultrasonic cleaner (200W 59Hz Shanghai Kedao Instrument Co., Ltd.).
[0033] Reagents: chrysophanol, emodin, liquiritin, forsythin, baicalin and berberine hydrochloride reference substances (China Institute for the Control of Biological Drugs); Niuhuang Ninggong Tablets (Shenyang Hongyao Pharmaceutical Co., Ltd.); , the water is ultrapure water.
[...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses a method for simultaneous determination of six active components consisting of chrysophanol, emodin, liquiritin, forsythin, baicalin and berberine hydrochloride in a Niuhuang Ninggong tablet through HPLC. Chromatographic conditions employed in the invention are as follows: a chromatographic column is TC-C18 (4.6 mm * 250 mm, 5 [mu]m); detection wavelength is 280 nm; a mobile phase is methanol-0.05% phosphoric acid; gradient elution comprises three parts, i.e., elution with methanol with a concentration varying in a range of 10 to 80% in the time period from 0 min to 35 min, then elution with methanol with a concentration of 80% in the time period from 35 to 50 min, and finally elution with methanol with a concentration varying in a range of 80 to 10% in the time period from 50 to 60 min; flow velocity is 1.0 mL / min; column temperature is 25 DEG C; and sample size is 10 [mu]L. Under the above-mentioned chromatographic conditions, chromatographic peaks are perfectly separated, and concentrations and peak areas of chrysophanol, emodin, liquiritin, forsythin, baicalin and berberine hydrochloride show good linear relation. The method is simple, rapid and accurate, has good repeatability and can provide quality bases for comprehensive evaluation and control of the Niuhuang Ninggong tablet.
Description
technical field [0001] The invention relates to a method for detecting active components in the Chinese patent medicine Niuhuang Ninggong Tablet, specifically a method for simultaneously measuring six active components in the Niuhuang Ninggong Tablet, which belongs to the technical field of medicine. Background technique [0002] Niuhuang Ninggong Tablets are used for exogenous febrile diseases, high fever, coma, convulsions, dizziness due to liver yang, tinnitus, headache, restlessness, insomnia, and epilepsy. It has a certain anti-relapse effect on schizophrenia. [0003] Niuhuang Ninggong Tablets is a medicine made by extracting and processing 27 herbs such as bezoar, dandelion, realgar, forsythia, rhubarb, borneol, honeysuckle, licorice, coptis, scutellaria, uncaria, rhubarb magnetite (calcined), scrophulariaceae, and kudzu root. . The preparation is included in WS3-B-2852-98 on the 36th page of the 15th volume of the Chinese Ministry of Health's drug standard tradition...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more