Preparation method of traditional Chinese medicine chemical component

A technology of licorice medicinal material, liquiritin, which is applied in the field of medicine to achieve the effects of easy standardization, low production cost and strong practicability

Inactive Publication Date: 2014-06-04
TIANJIN INSTITUTE OF PHARMA RESEARCH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The invention is novel in technology, easy to operate, energy-saving and environment-friendly, and has good practicability. At present, there is no relevant patent or literature report

Method used

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  • Preparation method of traditional Chinese medicine chemical component
  • Preparation method of traditional Chinese medicine chemical component
  • Preparation method of traditional Chinese medicine chemical component

Examples

Experimental program
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Effect test

Embodiment 1

[0044] (1) Take 15Kg of licorice, pulverize it, use 60% ethanol aqueous solution, heat and reflux to extract twice, add 120L for the first time, extract for 2 hours, and add 90L for the second time, reflux for 1 hour, filter, and depressurize the extract Concentrate to a relative density of 1.05 (60°C) clear paste, weighing 10.5 kg, add 40 L of water to the clear paste, heat to dissolve, filter to obtain the filtrate.

[0045] (2) Pass the filtrate through a YDW-04 macroporous adsorption resin column with a diameter of 20cm, and elute with water until the eluate is nearly colorless, then elute with 30% ethanol solution 5 times the column volume, and collect 30% The ethanol eluate was concentrated to a small volume (8L) under reduced pressure, left at room temperature for 30 hours, white crystals precipitated, filtered, rinsed with a small amount of 20% ethanol water, and dried to obtain 140.6g of crude liquiritin.

[0046] (3) Take the above crude liquiritin, add 3L of 60% ace...

Embodiment 2

[0049] (1) Take the licorice medicinal material, crush it, heat and reflux with 15 times of 60% ethanol aqueous solution to extract twice, the first time is 8 times the amount, extract for 2 hours, the second time is 7 times, extract for 1 hour, filter, and the extract is decompressed Concentrate to a relative density of 1.09 (60°C) clear paste, weighing 11.5kg, add 4 times the amount of water to the clear paste, heat to dissolve, filter to obtain the filtrate.

[0050] (2) Pass the filtrate through a HPD300 macroporous adsorption resin column, elute with water until the eluate is nearly colorless, then elute with 50L of 40% ethanol solution, collect the 40% ethanol eluate, and Concentrate under reduced pressure to a small volume (6 L), leave at room temperature for 20 hours, white crystals precipitate out, filter, rinse with a small amount of 20% ethanol in water, and dry to obtain crude liquiritin.

[0051] (3) Take the above crude product of liquiritin, add 5L of 60% ethano...

Embodiment 3

[0054] (1) Take 15Kg of licorice, pulverize it, use 30% ethanol aqueous solution, heat and reflux to extract twice, add 120L for the first time, extract for 2 hours, and add 90L for the second time, reflux for 1 hour, filter, and decompress the extract Concentrate to a relative density of 1.15 (60°C) clear paste, weighing 10.5 kg, add 40 L of water to the clear paste, heat to dissolve, filter to obtain the filtrate.

[0055] (2) Pass the filtrate through a YDW-04 macroporous adsorption resin column with a diameter of 20cm, and elute with water until the eluate is nearly colorless, then elute with 40L of 25% ethanol solution, and collect 25% ethanol to elute solution, concentrated under reduced pressure to a small volume (8 L), left at room temperature for 30 hours, white crystals precipitated, filtered, rinsed with a small amount of 20% ethanol water, and dried to obtain 140.6 g of crude liquiritin.

[0056] (3) Take the above crude liquiritin, add 3L of 40% acetone-water mixe...

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Abstract

The invention relates to a separation of a natural chemical component, and in particular to a method for extracting high-purity liquiritin from licorice medicinal material. The method mainly comprises the following steps: adding a proper amount of dilute ethanol into a licorice powder for extraction; concentrating the extract or percolating liquid; adding a proper amount of water for dissolution; filtering; separating by macroporous adsorption resin to obtain a liquiritin crude product; adding a proper amount of a solvent; heating for dissolving; filtering; standing for crystallization; filtering; and drying to obtain high-purity liquiritin. The product is determined by high performance liquid chromatography to have purity higher than 95%. The liquiritin produced by the technical scheme of the invention has high purity and large preparation volume; and the method has low cost, and stable and reliable process, and is especially suitable for separating and preparing of a large amount of high-purity liquiritin compound from the traditional Chinese medicine licorice.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for extracting and preparing high-purity liquiritin from licorice. Background technique [0002] Licorice is the roots and rhizomes of Glycyrrhiza Linn. in the family Leguminosae, Glycyrrhiza glabra, Glycyrrhiza glabra, and Glycyrrhiza inflate, which are distributed all over the world. Licorice has a long history of being used as medicine, and "Shen Nong's Materia Medica" listed it as the best medicine. There are many types of chemical components in licorice, mainly containing triterpenoid saponins and flavonoids. Liquiritin is the main component of total flavonoids in licorice and the main active ingredient in licorice. It has anti-depression, anti-oxidation, anti-arrhythmia, anti-ulcer, anti-cancer, anti-cancer, anti-tumor effects, and has a good development prospect . At present, there are many patents on the preparation methods of flavonoids and liqui...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/26C07H1/08
Inventor 刘素香陈常青刘毅白雪
Owner TIANJIN INSTITUTE OF PHARMA RESEARCH
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