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Application of betulinic acid serving as body cadmium scavenging agent

A betulinic acid and body technology, applied in the field of betulinic acid, can solve the problems of immune damage, limited efficiency, and inability to reduce cadmium residues in tissues, etc., and achieve the effects of broadening the application range, reducing cadmium residues, and having no toxic side effects in tissues

Active Publication Date: 2016-11-09
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

3) Prevention and treatment of nutrients such as zinc, iron, calcium and selenium, combined with specific proteins to antagonize the toxicity of cadmium, long-term intake of calcium will have mental side effects, excessive zinc will cause immune damage, iron acts on transport proteins, and is affected by the amount of protein Limitations; this method has limited efficiency in antagonizing cadmium and cannot reduce the residual amount of cadmium in tissues
[0005] At present, there is no report on the use of betulinic acid in the treatment of heavy metal cadmium poisoning

Method used

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  • Application of betulinic acid serving as body cadmium scavenging agent
  • Application of betulinic acid serving as body cadmium scavenging agent
  • Application of betulinic acid serving as body cadmium scavenging agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] [Example 1] Effect of Betulinic Acid on Cadmium Chloride-Induced Normal Cell Apoptosis

[0044] Through the CCK-8 cell proliferation experiment, the activity inhibition of cadmium chloride on three kinds of normal cells TM3, 293T and LO-2 cells was detected from the time gradient and concentration gradient, and the optimal dosage and action time of acute low-dose poisoning were determined, and then Use this time gradient to select the optimal concentration of betulinic acid for administration, and then use betulinic acid and cadmium chloride to jointly act on cells and perform flow detection.

[0045] 1. Effects of different doses of cadmium chloride on the proliferation of TM3, 293T and LO-2 cells

[0046] Three kinds of normal cells TM3, 293T and LO-2 cells were incubated with different concentrations of cadmium chloride solutions for 12h, 24h and 48h respectively, and then CCK-8 was used to detect cell viability. The results were as follows: figure 1 . As can be se...

Embodiment 2

[0051] [Example 2] Detection of betulinic acid side effects

[0052] There were 24 Kunming mice in four groups with 6 mice in each group, one group was blank control group, and the other three groups were 3 mg / kg, 10 mg / kg and 30 mg / kg three doses of betulinic acid groups, which were administered orally. Record the body weight of the mice every day, kill the mice 10 days later, weigh the organ weight, and obtain the organ coefficient, organ coefficient = (organ weight / mouse body weight)*1000; analyze biochemical data, detect HE staining, and determine betulinic acid Toxicity in mice at three doses.

[0053] 1. Effects of different doses of betulinic acid (BA) on body weight and organ coefficients of mice

[0054] From Figure 4 It can be seen that betulinic acid only slightly reduces the body weight of mice in the 30mg / kg dose, but has no effect on the organ coefficients of mouse liver, kidney and testis (organ coefficient increases, organ enlargement; organ coefficient dec...

Embodiment 3

[0062] [Example 3] Protective effect of betulinic acid pretreatment on cadmium poisoning mice

[0063] Five groups of 30 Kunming mice, 6 in each group, blank control group, cadmium chloride model group, and three groups of different doses of betulinic acid pretreatment groups, were administered orally. Cadmium chloride was injected intraperitoneally at a concentration of 1 mg / kg. Gastrointestinal treatment was carried out 1 hour in advance every day, followed by intraperitoneal injection for 10 consecutive days, and then starved for 24 hours, the mice were sacrificed, HE staining, graphite atomic absorption spectrometry was used to detect the residual amount of cadmium, and the data were analyzed. To determine the protective effect of betulinic acid pretreatment on cadmium-intoxicated mice.

[0064] 1. Effects of pretreatment with different doses of betulinic acid on body weight and organ coefficients of mice poisoned with cadmium chloride

[0065] From Figure 9 A It can b...

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Abstract

The invention relates to novel application of betulinic acid, in particular to application of betulinic acid in preparing drugs, health-care products and foods for clearing away cadmium in a body. It is verified through in-vitro and in-vivo tests that betulinic acid does not have obvious toxic and side effects on normal cells and mice, residues of cadmium in the mouse liver, kidney and testis are reduced, and discharge of cadmium in urine can be promoted; mouse liver, kidney and testis tissue damage caused by cadmium can be reduced. Betulinic acid has the effect of treating body cadmium accumulation and is safe and effective, betulinic acid is superior to a traditional therapeutic drug, the application range of betulinic acid is widened, and a new way is provided for treating heavy metal cadmium poisoning.

Description

technical field [0001] The invention relates to a new application of betulinic acid, in particular to the application of betulinic acid in the preparation of medicine for eliminating cadmium in the body. Background technique [0002] Cadmium is a silver-white shiny metal, and cadmium is not an essential element for the human body. Cadmium in the human body is absorbed from the external environment after birth. There are four main ways for ordinary people to be exposed to cadmium: air, water, food and tobacco. 80% of food cadmium comes from cereals and vegetables, and these foods come from cadmium-rich soil; in addition, because tobacco can selectively absorb cadmium from soil, the blood cadmium concentration of smokers is significantly higher than that of non-smokers. After entering the body, cadmium is very toxic to the human body due to its biological half-life of 10-30 years and extremely low excretion rate. About 50% of the cadmium in the body is enriched in the liver ...

Claims

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Application Information

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IPC IPC(8): A61K31/56A61P39/02A23L33/00
CPCA23V2002/00A61K31/56A23V2200/30
Inventor 杨芳樊荣罗大级胡鹏超王莹
Owner WUHAN UNIV
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