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Extraction method of globefish testis milt components

An extraction method and protamine technology, applied in the field of biological preparations, can solve the problems of limited extraction raw materials, complex protamine and protamine DNA extraction methods, and inability to effectively realize industrialization, etc., so as to improve utilization and reduce waste. effect used

Inactive Publication Date: 2020-02-07
广州市藻德道科技有限公司
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Problems solved by technology

[0007] In view of the above technical problems, the embodiment of the present invention provides a method for extracting protamine components of puffer fish testis, so as to solve the problem that the existing puffer fish protamine and protamine DNA extraction methods are complicated, the extraction raw materials are limited, and industrialization cannot be effectively realized. question

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  • Extraction method of globefish testis milt components
  • Extraction method of globefish testis milt components
  • Extraction method of globefish testis milt components

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Embodiment Construction

[0026] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative efforts fall within the protection scope of the present invention.

[0027] It should be noted that when an element is said to be "fixed" to another element, it may be directly on the other element, or there may be one or more intervening elements therebetween. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or one or more intervening elements may be present therebetween. The terms "vertical", "horizontal", "left", "right", "upper", "lower", "inner", "outer", "bottom", et...

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Abstract

The embodiment of the invention discloses an extraction method of globefish testis milt components. The extraction method of the globefish testis milt components includes the steps: globefish testes are pretreated, and scattered testis cells are obtained; ultrasonication is conducted on the scattered testis cells, and filtration and separation are carried out to obtain white precipitate; a set volume of concentrated hydrochloric acid is added to dissolve the white precipitate, and then a solution is formed; the solution passes through an ultrafiltration membrane, and then a trapped liquid anda filtrate are obtained separately; extraction is conducted on the trapped liquid, and then milt nucleic acids are obtained; and extraction is conducted on the filtrate, and then milt proteins are obtained. According to the extraction method of the globefish testis milt components, by utilizing the globefish testes as an extraction raw material, a large quantity of globefish viscus wastes producedby a globefish culture industry can be effectively utilized. Besides, according to the technical scheme, separation and extraction of the milt DNA and the milt proteins are achieved, thus, the availability of testis tissue can be improved, usage of surface active agents and organic solvents is also reduced, and therefore the extraction method of the globefish testis milt components is more environmentally friendly.

Description

technical field [0001] The invention relates to the technical field of biological preparations, in particular to a method for extracting fish essence from puffer fish testis. Background technique [0002] Pufferfish is the collective name for fish in the family Fuguidae of the bony fish class, commonly known as puffer fish. It is a bottom fish in warm temperate and tropical coastal waters. It mainly inhabits the middle and lower layers of the ocean, and a few species enter freshwater rivers. When encountering external danger, the pufferfish will make the whole body float to the surface of the water in a spherical shape, and at the same time, the small spines on the skin will stand up to defend itself. The common ones are red-finned oriental puffer, dark-patterned puffer, black-gilled puffer, concave-nosed puffer, black-spotted puffer and so on. [0003] The puffer fish has delicious meat and high nutritional value, and has become the "king of fish" in people's mouth. Howev...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10C07K14/46C07K1/34
CPCC07K14/461C12N15/1003
Inventor 黄连生陈静
Owner 广州市藻德道科技有限公司
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