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Takifugu flavidus protamine extraction method

An extraction method and protamine technology are applied in the biological field to achieve the effects of improving the extraction rate and increasing the extraction purity

Inactive Publication Date: 2019-06-21
FISHERIES RES INST OF FUJIAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The commonly used protamine extraction method is mainly to use NaCl solution to dissolve, extract with sulfuric acid or hydrochloric acid, and then use cold ethanol precipitation to obtain it, but there is no report on extracting protamine from pufferfish.

Method used

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  • Takifugu flavidus protamine extraction method
  • Takifugu flavidus protamine extraction method
  • Takifugu flavidus protamine extraction method

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Experimental program
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Embodiment Construction

[0019] ,experimental method

[0020] 1.1 Composition and safety determination of fugu testis

[0021] Moisture is determined by direct drying method according to GB / T 5009.236-2016; crude protein is determined by Kjeldahl method according to GB 5009.5-2016; crude fat is determined by Soxhlet extraction method according to GB / T 5009.6-2016; ash content is determined by reference to GB / T5009. 4—2016 Determination by ignition weighing method. Tetrodotoxin (TTX) was tested according to the instruction manual of tetrodotoxin ELISA detection kit, and the evaluation criteria were: TTX ≥ 1 000 MU / g was highly toxic, recorded as grade A; 100~999 MU / g was highly toxic, recorded as grade B ; 10~99MU / g is mildly toxic, graded as C grade; <10 MU / g is non-toxic, graded as D grade, 1MU=0.18 μg / g TTX.

[0022] 1.2 Protamine extraction process

[0023] Weighed 10.00 g of dried testis of chrysanthemum yellow oriental puffer fish, added 100 mL of NaCl solution with different pH and concentrat...

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Abstract

The invention provides a takifugu flavidus protamine extraction method. Extraction process conditions include that pH of 0.14mol / L of NaCl solution is 8.5, the concentration of sulfuric acid solutionis 0.6mol / L, consumption of the sulfuric acid solution is 4.0 volumetric times of a precipitant, an extraction temperature is 45 DEG C, extraction time is 2.4h, consumption of ethyl alcohol is 4 volumetric times of supernatant, and the actual extraction rate of protamine is 19.07%. Extracting protamine from takifugu flavidus testes provides an experimental foundation for research on physicochemical properties and related functional characteristics of protamine, and a function of effective utilization of takifugu flavidus testis tissues can be achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting protamine from puffer chrysanthemum. Background technique [0002] Pufferfish (Tetraodontidae), commonly known as "puffer fish", belongs to Chordate Phylum, Fuguformes, Fuguidae. It is a high-protein, low-fat fish with high edible value. Wild pufferfish is highly poisonous. The testis (commonly known as milt) at the sexually mature stage of male puffer fish accounts for more than 7% of the whole male fish body mass. Except for the testis of Takifugu vermicularis which contains tetrodotoxin, the testes of other species basically do not contain it. In 2016, the Ministry of Agriculture and others jointly issued the "Notice on Conditionally Liberating the Processing and Operation of Redfin Pufferfish and Pufferfish Obscura"; Artificial culture of yellow puffer (Takifugu Flavidus) and two-spot puffer (Takifugubimaculatus). According to statistics, th...

Claims

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Application Information

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IPC IPC(8): C07K14/46C07K1/14
Inventor 刘淑集许旻苏永昌刘智禹
Owner FISHERIES RES INST OF FUJIAN
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