11 results about "Goatpox virus" patented technology

The present invention discloses a kit for direct amplification detection of goat pox virus based on single-domain antibody 9‑4‑2 immunomagnetic beads and its application. The kit includes single-domain antibody 9‑4‑2 immunomagnetic beads obtained by coupling the goat pox virus single-domain antibody 9‑4‑2 shown in SEQ ID NO.1 with streptavidin‑biotin and magnetic beads. The present invention uses the single-domain antibody 9‑4‑2 protein purified by gel cutting to couple with magnetic beads to obtain immunomagnetic beads. After the immunomagnetic beads are enriched with goat pox virus, the genomic RNA does not need to be extracted and can be directly amplified. The proposal of the present invention overcomes the phenomenon that goat pox virus is not easy to be found at the peak of virus replication due to the long time of forming the skin, the virus content of the collected diseased material is low, so the detection rate is low, and it is easy to miss the detection, etc.; as well as a series of problems such as time-consuming, costly, and laborious when extracting genomic DNA, and polluting the environment during operation, and it is efficient, sensitive, specific, stable, and practical. The proposal of the present invention provides an efficient technical means for the detection of goat pox virus disease.

The application belongs to the technical field of virus detection, and discloses a sheep disease virus multiplex PCR detection primer, method and application. The base sequence of the primer FMDV-F of the sheep foot-and-mouth disease virus is SEQ ID NO:1, the base sequence of the primer FMDV-R is SEQ ID NO:2; the base sequence of the primer ORFV-F of the sheep orf virus is SEQ ID NO:3, the base sequence of the primer ORFV-R is SEQ ID NO:4; the base sequence of the primer GTPV-F of the goat pox virus is SEQ ID NO:5, and the base sequence of the primer GTPV-R is SEQ ID NO:6. The application optimizes the multiplex PCR, determines the optimal reaction system and the optimal reaction procedure, and can accurately detect single or mixed infection of the sheep foot-and-mouth disease virus, the sheep orf virus and the goat pox virus, and has an important role in epidemiological investigation and epidemic disease prevention and control.

The invention belongs to the field of biology, and particularly relates to a recombinant goat pox virus as well as a construction method and application thereof. The method comprises the following steps: constructing a donor vector pGTPV-132LSDV-F2A-1322-P2A-GFP by utilizing a DNA (Deoxyribonucleic Acid) recombination technology, and then constructing a recombinant goat pox virus rGTPV-TK-750-1 (rGTPV-132) for stably expressing the LSDV 132 protein by taking a GTPV vaccine strain (AV41) as a framework and a TK gene as an insertion target spot through homologous recombination; the recombinant goat pox virus rGTPV-132 can stably express the LSDV 132 protein, has good hereditary stability and high virus titer, can be used as a strain for vaccine production, and lays a foundation for research, development and application of vaccines for preventing LSDV.

The invention provides an application of ciclopirox amine in preparation of an anti-poxvirus medicine, and belongs to the technical field of biological medicines. The invention relates to an application of ciclopirox olamine in preparation of anti-poxvirus drugs. It is proved for the first time that ciclopirox amine has efficient anti-poxvirus activity, can inhibit orthopoxvirus and goat poxvirus from infecting host cells, and meanwhile inhibits poxvirus replication. Therefore, the ciclopirox olamine can be applied to treatment of diseases related to poxvirus infection and drug development. The invention opens up a new way for the application of ciclopirox olamine, and provides a new means for treating poxvirus infection related diseases in the fields of breeding industry and biomedicine.

The invention relates to the technical field of bioengineering, in particular to a hybridoma cell strain, a goat pox virus G9 protein monoclonal antibody and application. The hybridoma cell strain is named as 9C5, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.46312. Goat pox virus G9 protein immunogen is prepared, hybridoma cells capable of stably secreting antibodies are prepared through cell fusion and multi-time subcloning, a large number of goat pox virus G9 protein monoclonal antibodies are obtained through ascites preparation, the goat pox virus G9 protein monoclonal antibodies can still be effectively combined with goat pox viruses after being diluted by 64K times, and the goat pox virus G9 protein monoclonal antibodies can be used for detecting the goat pox viruses. The method can be used for constructing a goat pox virus detection method and lays a foundation for prevention and treatment of goat pox viruses.

This invention provides the application of ciclopiroxamide in the preparation of anti-poxvirus drugs, belonging to the field of biomedical technology. The application of ciclopiroxamide in the preparation of anti-poxvirus drugs. This invention demonstrates for the first time that ciclopiroxamide possesses highly efficient anti-poxvirus activity, inhibiting the infection of host cells by orthopoxvirus and goatpoxvirus genera, while simultaneously inhibiting poxvirus replication. Therefore, ciclopiroxamide can be used in the treatment and drug development of poxvirus-related diseases. This invention opens up new avenues for the use of ciclopiroxamide, providing a new means for the treatment of poxvirus-related diseases in the livestock industry and biomedical fields.

The application belongs to the technical field of biological detection, and discloses a capripoxvirus specific competitive ELISA detection kit and application thereof. The application discloses a hybridoma cell strain GTPV-N1P-4H6, and realizes high-efficiency soluble expression of GTPV-N1 based on a prokaryotic expression system. After immunizing mice with GTPV-N1 as an immunogen, a GTPV positive hybridoma cell strain GTPV-N1P-4H6 capable of stably secreting antibodies is successfully prepared and screened after cell fusion. The ELISA titer of the antibody obtained after the monoclonal antibody secreted by the cell strain is purified is 1:64000, the heavy chain of the antibody is IgGa2 subtype, and the light chain is Kappa chain. Based on the antibody, high-efficiency detection of capripoxvirus N1 protein can be realized.

The invention discloses a primer probe group and a kit for detecting animal pox viruses and application of the primer probe group and the kit. The invention provides a primer probe set for detecting animal pox viruses. The primer probe set comprises any one of primer probe sets 1-5. The animal pox disease TaqMan qPCR detection method established by the invention has a good linear relationship. The method has no cross reaction with various pathogens such as goat pox virus and the like, and the specificity reaches 100%. The detection lower limits of MPXV, SWPV, GTPV, FWPV and LSDV are respectively 1.84 copies / [mu] L, 2.24 * 10 < 1 > copies / [mu] L, 1.34 copies / [mu] L, 3.70 copies / [mu] L and 3.07 copies / [mu] L, and the sensitivity is improved by one order of magnitude compared with a reference method. The stability and the repeatability are good.

The application discloses a kind of based on single-domain antibody 9-4-1 immunomagnetic beads direct spread detection goat poxvirus kit and its application.The kit includes the single-domain antibody 9-4-1 immunomagnetic beads obtained by the single-domain antibody 9-4-1 shown in SEQ ID NO.1 goat poxvirus is coupled with magnetic bead by streptavidin-biotin.The single-domain antibody 9-4-1 protein purified by gel cutting is coupled with magnetic bead to obtain immunomagnetic beads, and after goat poxvirus is enriched, it can be directly amplified without extracting genomic RNA.The application overcomes the phenomenon that goat poxvirus is not easy to be found at virus replication peak period due to long time of forming sandwich, virus content is low when collecting disease material, detection rate is low, and a series of problems such as easy to miss detection;And it is time-consuming, costly and laborious to extract genomic DNA, and a series of problems such as environmental pollution during operation, and efficient, sensitive, specific, stable and practical, and the application provides an efficient technical means for detection of goat poxvirus disease.

The application discloses a specific goatpox virus P32 truncated protein and application thereof in vaccine efficacy detection. The amino acid sequence of the protein is shown as SEQ ID NO: 3. The specific truncated form (aa1-aa238) has significantly better soluble expression in E. coli than the full-length and other truncated forms (such as aa1-aa277). A competitive ELISA method for quantitatively detecting the efficacy of bovine nodular skin disease inactivated vaccine is established by taking the specific truncated form as a coating antigen. The method is systematically optimized, and has high sensitivity, strong specificity and good repeatability. Key is that the relative efficacy (RP) value measured by the method is highly correlated with animal challenge protection results, and when the RP value is greater than or equal to 1.0, complete immune protection can be predicted, so that the traditional animal challenge test can be reliably replaced. Compared with the immune challenge method, the detection period is greatly shortened (from 63 days to 2 days), target animals are not used, the detection cost is saved, and the method does not need P3 laboratory conditions and has no biosafety risk.

This invention discloses a single-primer dual-fluorescence detection kit for identifying sheep poxvirus and bovine nodular dermatovirus, along with its dedicated primers and probes, belonging to the field of biological technology. The kit provided by this invention includes universal PCR amplification primers for detecting sheep poxvirus, a probe for detecting sheep poxvirus, and a probe for detecting bovine nodular dermatovirus. It can be used for direct qualitative identification and detection of sheep poxvirus and bovine nodular dermatovirus within the genus *Capepoxvirus*, exhibiting advantages such as high sensitivity, strong specificity, and good repeatability, and can also achieve accurate quantification. This invention can provide technical support and assistance for the identification and differentiation of *Capepoxvirus* viruses, epidemiological investigations, virus control, and the purification and screening of vaccine raw materials.