6 results about "Goatpox virus" patented technology

The application belongs to the technical field of virus detection, and discloses a sheep disease virus multiplex PCR detection primer, method and application. The base sequence of the primer FMDV-F of the sheep foot-and-mouth disease virus is SEQ ID NO:1, the base sequence of the primer FMDV-R is SEQ ID NO:2; the base sequence of the primer ORFV-F of the sheep orf virus is SEQ ID NO:3, the base sequence of the primer ORFV-R is SEQ ID NO:4; the base sequence of the primer GTPV-F of the goat pox virus is SEQ ID NO:5, and the base sequence of the primer GTPV-R is SEQ ID NO:6. The application optimizes the multiplex PCR, determines the optimal reaction system and the optimal reaction procedure, and can accurately detect single or mixed infection of the sheep foot-and-mouth disease virus, the sheep orf virus and the goat pox virus, and has an important role in epidemiological investigation and epidemic disease prevention and control.

The application belongs to the technical field of biological detection, and discloses a capripoxvirus specific competitive ELISA detection kit and application thereof. The application discloses a hybridoma cell strain GTPV-N1P-4H6, and realizes high-efficiency soluble expression of GTPV-N1 based on a prokaryotic expression system. After immunizing mice with GTPV-N1 as an immunogen, a GTPV positive hybridoma cell strain GTPV-N1P-4H6 capable of stably secreting antibodies is successfully prepared and screened after cell fusion. The ELISA titer of the antibody obtained after the monoclonal antibody secreted by the cell strain is purified is 1:64000, the heavy chain of the antibody is IgGa2 subtype, and the light chain is Kappa chain. Based on the antibody, high-efficiency detection of capripoxvirus N1 protein can be realized.

The invention discloses a primer probe group and a kit for detecting animal pox viruses and application of the primer probe group and the kit. The invention provides a primer probe set for detecting animal pox viruses. The primer probe set comprises any one of primer probe sets 1-5. The animal pox disease TaqMan qPCR detection method established by the invention has a good linear relationship. The method has no cross reaction with various pathogens such as goat pox virus and the like, and the specificity reaches 100%. The detection lower limits of MPXV, SWPV, GTPV, FWPV and LSDV are respectively 1.84 copies / [mu] L, 2.24 * 10 < 1 > copies / [mu] L, 1.34 copies / [mu] L, 3.70 copies / [mu] L and 3.07 copies / [mu] L, and the sensitivity is improved by one order of magnitude compared with a reference method. The stability and the repeatability are good.

The application discloses a kind of based on single-domain antibody 9-4-1 immunomagnetic beads direct spread detection goat poxvirus kit and its application.The kit includes the single-domain antibody 9-4-1 immunomagnetic beads obtained by the single-domain antibody 9-4-1 shown in SEQ ID NO.1 goat poxvirus is coupled with magnetic bead by streptavidin-biotin.The single-domain antibody 9-4-1 protein purified by gel cutting is coupled with magnetic bead to obtain immunomagnetic beads, and after goat poxvirus is enriched, it can be directly amplified without extracting genomic RNA.The application overcomes the phenomenon that goat poxvirus is not easy to be found at virus replication peak period due to long time of forming sandwich, virus content is low when collecting disease material, detection rate is low, and a series of problems such as easy to miss detection;And it is time-consuming, costly and laborious to extract genomic DNA, and a series of problems such as environmental pollution during operation, and efficient, sensitive, specific, stable and practical, and the application provides an efficient technical means for detection of goat poxvirus disease.

The application discloses a specific goatpox virus P32 truncated protein and application thereof in vaccine efficacy detection. The amino acid sequence of the protein is shown as SEQ ID NO: 3. The specific truncated form (aa1-aa238) has significantly better soluble expression in E. coli than the full-length and other truncated forms (such as aa1-aa277). A competitive ELISA method for quantitatively detecting the efficacy of bovine nodular skin disease inactivated vaccine is established by taking the specific truncated form as a coating antigen. The method is systematically optimized, and has high sensitivity, strong specificity and good repeatability. Key is that the relative efficacy (RP) value measured by the method is highly correlated with animal challenge protection results, and when the RP value is greater than or equal to 1.0, complete immune protection can be predicted, so that the traditional animal challenge test can be reliably replaced. Compared with the immune challenge method, the detection period is greatly shortened (from 63 days to 2 days), target animals are not used, the detection cost is saved, and the method does not need P3 laboratory conditions and has no biosafety risk.

This invention discloses a single-primer dual-fluorescence detection kit for identifying sheep poxvirus and bovine nodular dermatovirus, along with its dedicated primers and probes, belonging to the field of biological technology. The kit provided by this invention includes universal PCR amplification primers for detecting sheep poxvirus, a probe for detecting sheep poxvirus, and a probe for detecting bovine nodular dermatovirus. It can be used for direct qualitative identification and detection of sheep poxvirus and bovine nodular dermatovirus within the genus *Capepoxvirus*, exhibiting advantages such as high sensitivity, strong specificity, and good repeatability, and can also achieve accurate quantification. This invention can provide technical support and assistance for the identification and differentiation of *Capepoxvirus* viruses, epidemiological investigations, virus control, and the purification and screening of vaccine raw materials.