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Orf F1L vaccine based on ferritin nanoparticles and preparation method of orf F1L vaccine

A nanoparticle, aphthous canker technology, applied in the field of immune vaccines, can solve the problems of low antibody level, short immune protection period, inability to completely eliminate and control, and achieve the effects of improving immune function, rapid preparation and easy preparation

Active Publication Date: 2019-11-22
SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

The main means of clinical prevention of aphthous ulcers is to vaccinate. Commonly used are inactivated vaccines and cell attenuated vaccines. However, there are disadvantages such as low antibody level and short immune protection period, so it is not suitable for mass production and storage.
At present, there is no effective treatment for the disease, and only clinical trauma treatment and supportive therapy can be adopted. In terms of prevention and control, at home and abroad, the main method of prevention is to inoculate the attenuated live oral vaccine, but this vaccine still cannot completely eliminate and control the disease. There are certain defects in immunity and safety, therefore, it is imperative to create safe and efficient new vaccines, and the research of new genetic engineering vaccines is of great significance

Method used

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  • Orf F1L vaccine based on ferritin nanoparticles and preparation method of orf F1L vaccine
  • Orf F1L vaccine based on ferritin nanoparticles and preparation method of orf F1L vaccine
  • Orf F1L vaccine based on ferritin nanoparticles and preparation method of orf F1L vaccine

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Embodiment 1

[0037] This embodiment relates to a kind of oral ulcer F1L vaccine based on ferritin nanoparticles and its preparation method; the specific steps are as follows:

[0038] Step 1), construction of pET32a-F1L / Fe expression engineering bacteria:

[0039] According to the published aphthus F1L gene sequence on GenBank, remove its transmembrane region (TMH) and design PCR primers for amplifying the F1L truncated gene. The amplified fragment size is 873bp. Fusion PCR primers were designed to link F1L and Fe through the linker protein (SGG), and EcoR I and EcoR V restriction sites were added to both ends of the upstream and downstream primers, respectively. Primer sequences are shown in Table 1:

[0040] Table 1

[0041]

[0042] Primers were synthesized by Shanghai Qingke Biotechnology Co., Ltd.

[0043] First, use the ORFV genome as a template to perform PCR amplification with the first pair of primers, recover the PCR amplified fragment, connect it to the PMD-19T vector, obt...

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Abstract

The invention discloses an orf F1L vaccine based on ferritin nanoparticles and a preparation method of the orf F1L vaccine. According to the invention, a fusion protein is characterized in that an orfF1L protein is externally connected to the surface of ferritin through a genetic engineering method to form nanoparticles, namely the orf F1L / Fe fusion protein. The target protein provided by the invention has good immunogenicity and can be used for preventing infection of the orf virus.

Description

technical field [0001] The invention belongs to the technical field of immune vaccines, and in particular relates to a mouth ulcer F1L vaccine based on ferritin nanoparticles and a preparation method thereof. Background technique [0002] The physical and chemical characteristics of nanoparticle materials endow them with many unique biological properties, which are widely used in the research and development of vaccines and drugs. Ferritin is an iron storage protein widely present in organisms, which can self-assemble into nanoparticles and is a hotspot in the field of bionanomaterials research. In vaccine development, ferritin is an ideal antigen carrier. Masaru Kanekiyo et al. fused and expressed the HA protein of influenza virus H1N1 at the N-terminus of Helicobacter pylori ferritin to form a nano-vaccine with 8 HA trimers on the surface, which can induce 10 times the inactivated trivalent influenza virus vaccine. The enhancement of the above antibody response can also ...

Claims

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Application Information

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IPC IPC(8): A61K39/275A61K39/385A61P31/20C07K14/065C12N15/66C12N15/70
CPCA61K39/12A61K39/385A61P31/20C07K14/005C12N15/66C12N15/70C12N2710/24222C12N2710/24234A61K2039/552A61K2039/6031
Inventor 刘光清邢雪缪秋红朱杰李传锋陈宗艳
Owner SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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