Kit for detecting orf virus of sheep and detection method thereof

Abstract

The invention provides a detection method for detecting the orf virus of sheep. Real-time fluorescent quantitative PCR (polymerase chain reaction) detection is performed on a DNA (deoxyribonucleic acid) template of a sample by designing a pair of primers and a probe. The invention further provides a kit for detecting the orf virus of the sheep by utilizing the detection method. The real-time quantitative method which is established against H2L genes in a conserved region of the ORFV (orf virus) is high in specificity and good in stability. The kit for detecting the orf virus of the sheep, provided by the invention, is simple and convenient to operate, a user only needs to add DNA of the sample to be detected into a reaction tube, detection results can be obtained according to an amplification curve, the operation is time-saving and labor-saving, the cost is lower, and the time is shortened from original 3-4 hours to 1-2 hours; and furthermore, the sensitivity is higher than that of the ordinary PCR, and the detection method and the kit can be used for detecting the low content of the orf virus of the sheep, can be suitable for all levels of prevention and control units, basic-level veterinary stations, large and medium-sized breeding farms and the like in China, and has better application prospects.

Description

Technical field [0001] The invention relates to a TaqMan Real-time kit for detecting sheep infectious pustular dermatitis virus and a detection method thereof, in particular to a kit for detecting sheep infectious pustular dermatitis virus by TaqMan real-time fluorescent quantitative PCR and a detection method thereof. Background technique [0002] Sheep infectious impetigo dermatitis virus is abbreviated as "Orf Virus" (Orf Virus, ORFV), which can cause contact infections between sheep and humans, and even cause the development of sheep breeding and environmental safety issues. The environment contaminated by ORFV poses a high risk of infection for young sheep and lactating ewes. They mainly attack the skin or mucosa of the host, and can lead to serious persistent infections and secondary infections. ORFV has distinct species characteristics and a wealth of functional genes. Its genome is a double-stranded DNA with closed ends, about 138 kb in length, a core region (88 kb) in th...

AI Technical Summary

Problems solved by technology

From an economic point of view, the price of foreign testing reagents for testing a sample is often tens of yuan or even hundreds of yuan, which is too high for sheep farmers to accept

At present, there is only a common PCR kit for the detection of sheep infectious pyoderma virus. The operation of this kit is relatively complicated, and the results must be determined by electrophoresis strips, which is time-consuming and laborious, and the ethidium bromide added to the gel during electrophoresis is harmful to human health. , take extra care when operating

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