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Isolation medium of genitourinary tract mycoplasma Un and Mh and application of Un and Mh isolation medium

A genitourinary tract, separation medium technology, applied in the direction of microorganisms, microorganism-based methods, biochemical equipment and methods, etc., can solve problems such as different components, achieve the effect of preventing harmful effects, easy comparison, and intuitive gold standard results

Active Publication Date: 2015-06-10
众爱生河北生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0020] Since the growth environment of the Ureaplasma urealyticum medium and the Mycoplasma hominis medium are different, and the components required for growth are different, there is currently no medium that can simultaneously cultivate Ureaplasma urealyticum and Mycoplasma hominis

Method used

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  • Isolation medium of genitourinary tract mycoplasma Un and Mh and application of Un and Mh isolation medium
  • Isolation medium of genitourinary tract mycoplasma Un and Mh and application of Un and Mh isolation medium
  • Isolation medium of genitourinary tract mycoplasma Un and Mh and application of Un and Mh isolation medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A kind of urogenital mycoplasma Uu, Mh separation culture medium, every 1000ml culture medium contains following composition:

[0052]

[0053] Additives: DMEM 50ml, self-made biological factor 5ml;

[0054] Pure water was added to make up to 1000ml.

[0055] Among them, the self-made biological factor is poultry egg yolk extract, which contains vitamin A, organic selenium, trace amounts of unsaturated fatty acids and phospholipids, and 10-25v / v% ethanol, of which vitamin The content of A is 2-10u / ml, and the content of organic selenium is 0.2-2.0ppm.

[0056] Poultry egg yolk extract The preparation method of the poultry egg yolk extract is as follows: (1) take fresh selenium-enriched eggs and soak them in 70v / v% ethanol solution for 30 minutes; Put the egg yolk in a sterile flask; (3) Add 90v / v% ethanol to the sterile flask containing the egg yolk at a weight ratio of 1:2, stir and mix well, and place it at 4°C to 8°C after closing the bottle mouth. Store at ℃ f...

Embodiment 2

[0059] A kind of urogenital mycoplasma Uu, Mh separation culture medium, every 1000ml culture medium contains following composition:

[0060]

[0061]

[0062] Additives: DMEM 150ml, self-made biological factor 15ml; ampicillin 1 million units, natamycin 50mg;

[0063] Pure water was added to make up to 1000ml.

[0064] The preparation of self-made biological factors is the same as that in Example 1.

[0065] The pH of the medium is adjusted by dilute acid and alkali, so that the pH of the medium is 6.0-6.2, and the medium is stored at 7°C-8°C.

Embodiment 3

[0067] A kind of urogenital mycoplasma Uu, Mh separation culture medium, every 1000ml culture medium contains following composition:

[0068]

[0069] Additives: DMEM 100ml, self-made biological factor 10ml, ampicillin 1 million units, natamycin 50mg;

[0070] Pure water was added to make up to 1000ml.

[0071] The preparation of self-made biological factors is the same as that in Example 1.

[0072] The pH of the medium is adjusted by dilute acid and alkali so that the pH of the medium is 6.1-6.2, and the medium is stored at 9°C-10°C.

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Abstract

The invention relates to an isolation medium of genitourinary tract mycoplasma Un and Mh and application of the isolation medium. Each 1,000ml of culture medium contains the following components: 15g of peptone, 15g of tryptone, 5g of sodium chloride, 5g of urea, 5g of arginine, 10ml of 0.4% phenol red, 50ml of bovine serum, 50-150ml of DMEM, 5-15ml of self-made biological factor and pure water added to a constant volume of 1,000ml. Compared with the prior art, according to the culture medium disclosed by the invention, due to the addition of an additive, the performance and the selectivity of the culture medium are improved; the nutrients are relatively abundant; the growth and reproduction requirements of Uu and Mh are easily met; and the result is observed and reported a day earlier in comparison with a traditional culture medium in general condition.

Description

technical field [0001] The invention relates to a culture medium, in particular to a culture medium for separating Uu and Mh of urogenital mycoplasma and its application. Background technique [0002] Mycoplasma (Mycoplasma) is a class of minimal prokaryotic microorganisms that lack cell walls, are highly pleomorphic, can pass through 0.22um bacteria filters, and can grow and reproduce in non-living media. Mycoplasma was first isolated by Nocard et al. in 1898, officially named Mycoplasma in 1956, established a new independent class in microbial classification - Mollicutes, and has rapidly become An independent branch of microbiology, namely Mycoplasmology (Mycoplasmology). [0003] Mycoplasma is a microorganism between viruses and bacteria, and more than 150 species have been isolated so far. The main ones related to human diseases are Mycoplasma pneumoniae (Mp), Ureaplasma urealyticum (Uu for short) and Mycoplasma hominis (Mh for short). Uu and Mh are internationally re...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/35
CPCC12N1/20
Inventor 文凤岐石楠武济民孙亚洲肖家祁
Owner 众爱生河北生物科技有限公司
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