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Method of detecting infection with urogenital mycoplasmas in humans and a kit for diagnosing same

A technique for urogenital tract and mycoplasma infection, which is applied in the field of human urogenital tract mycoplasma infection detection and diagnostic kits

Inactive Publication Date: 2010-03-17
MOR RES APPL LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As known to those skilled in the art, available techniques suffer from various drawbacks including, for example, limited sensitivity and limited specificity (occurrence of false positive or false negative results)

Method used

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  • Method of detecting infection with urogenital mycoplasmas in humans and a kit for diagnosing same
  • Method of detecting infection with urogenital mycoplasmas in humans and a kit for diagnosing same
  • Method of detecting infection with urogenital mycoplasmas in humans and a kit for diagnosing same

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Multiple samples were taken from multiple patients with acute or chronic urogenital infections. Mycoplasma strains were grown from each sample and the presence of mycoplasma was verified by PCR. Six samples in which mycoplasma had been confirmed by PCR to belong to U. urealyticum or U. minumum were cultured in 10B liquid medium containing 7.5% FCS (fetal calf serum). Cells were centrifuged at 27,000 xg for 40 minutes and washed twice with phosphate buffered saline. The washed cell pellet was sonicated in the presence of 1 mM benzenemethanesulfonyl fluoride (a protease inhibitor), and the sonicated mass containing all cellular components (i.e., unfractionated mixture of proteins, lipoproteins, and nucleic acids) was washed with As mycoplasma antigen.

Embodiment 2

[0032] A mixture of ureaplasma antigens from 6 different ureaplasma species strains (3 ureaplasma minutiae (Up) and 3 ureaplasma urealyticum (Uu)) was prepared. Strains were isolated from patients as described in Example 1 and designated as "new". The identity of the species was verified by PCR. Serum samples from 113 patients with different diseases, which had been previously analyzed in our laboratory, were tested by ELISA with the "new" pool of antigens. In parallel, U. urealyticum ATCC #27815 from the previously named U. urealyticum serotype 3 (Up) and the U. urealyticum ATCC # previously named U. urealyticum serotype 4 (Uu) were used. 27816 prepared antigens were subjected to ELISA. Use each of them separately, and designate both here as "old". These antigens were used to test the 113 serum samples. Thus, ELISA tests based on three different antigen systems were compared.

[0033] Several serotypes were used, recorded in this laboratory as: a) Up positive, Uu positiv...

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Abstract

A method and a kit are provided for detecting an infection by mycoplasma, particularly an urogenital infection in humans. The presence of specific anti-mycoplasma antibodies in a biological sample ofa diagnosed subject is detected by reaction with an immobilized mixture of various antigenic determinants associated with a variety of pathological states.

Description

technical field [0001] The present invention relates to the detection of infections caused by mycoplasma, especially infections of the human genitourinary tract. The present invention provides kits for diagnosing urogenital tract infections caused by a wide range of Mycoplasma strains. Background technique [0002] Mycoplasma are a unique group of bacteria that often cause insidious infections in humans, animals and plants. In animals, they are notorious as pathogens of morbidity and mortality. In humans, many disorders are associated with mycoplasma infection, an example being mycoplasma pneumonia or common sexually transmitted infections, the exact diagnosis of which remains difficult [see eg Merck Manual, 17th edition, (1999) p. 1326]. An increasing body of evidence related to mycoplasma pathogenesis is accumulating in the literature, but there are no generally available well-established tests that would significantly facilitate the analysis of urogenital mycoplasma dis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
CPCG01N2469/20G01N33/56933
Inventor 舒拉米斯·霍罗威茨
Owner MOR RES APPL LTD
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