Mycoplasma bovis diagnosis reagent and its application

Abstract

The invention relates to the diagnostic medicine of animals, especially relates to a mycoplasma bovis diagnosis technology, and concretely relates to a multi-epitope fusion antigen having an amino acid sequence represented by SEQ ID NO:1 or SEQ ID NO:2, and its application in the preparation of a mycoplasma bovis diagnosis reagent. The diagnosis reagent can be used as a solid phase vector coating antigen of an indirect ELISA kit and is combined with its specific antibody, a horseradish peroxidase coupled anti-cattle IgG antibody is added and incubated, and a color development reaction is carried out, and the color development degree is proportional to the amount of the anti-mycoplasma bovis antibody in a sample to be measured. The technology has the advantages of simple operation, no need of complex equipment, low technical requirements on the laboratorial conditions and experiment personals, low detection cost, and suitableness for the large-scale development in the basic level and the culture farm; the multi-epitope fusion antigen has a low making cost and is suitable for large-scale application; and has the advantages of high sensitivity and specificity, small batch difference, and high detection result consistence because of the adoption of multi-epitope as a target.

Description

technical field [0001] The invention relates to animal diagnostic medicine, in particular to the diagnosis technique of Mycoplasma bovis pneumoniae. Background technique [0002] Mycoplasma bovis (MB) belongs to the kingdom of prokaryotes, Firmicutes, Mollicula, Mycoplasma, Mycoplasma family, Mycoplasma genus, and is the smallest and simplest prokaryote that can replicate autonomously. In the 1960s, it was first reported that Mycoplasma bovis caused pneumonia and mastitis in cattle. After that, people found that MB could also cause arthritis, keratitis, otitis, reproductive tract inflammation, abortion and other diseases in cattle. According to foreign reports, at least a quarter of the annual economic loss caused by bovine respiratory diseases is caused by Mycoplasma bovis, especially in the cattle transportation link, the incidence rate of the whole herd is as high as 90%, and the duration lasts for more than three months. affect the growth and development of cattle. At ...

AI Technical Summary

Problems solved by technology

For Mycoplasma bovis, a more prominent problem is that due to the relative lag in the basic research of Mycoplasma bovis, the advantage and conserved antigens that induce the body to produce antibodies after infection with this pathogen have not yet been clarified—that is, the rapid induction of antibodies and the The duration of antibody production is long, and the antigen is not easy to mutate during infection

For example, although the reported VSP has strong immunogenicity, it is prone to variation and affects the accuracy of detection.

Therefore, if a specific pathogen protein is prepared as a capture antigen using the traditional technical route, the detection result may not accurately reflect the real infection of the pathogen.

Images