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Immunodetection kit for mycoplasma pneumoniae antibody IgG, and preparation method and use method thereof

A Mycoplasma pneumoniae and immune detection technology, applied in the field of immune analysis, can solve the problems of ELISA unsatisfactory, shorten patient waiting time, and long time-consuming, so as to reduce detection background interference and non-specific binding, improve intensity and sensitivity, and improve detection speed effect

Pending Publication Date: 2020-06-05
JIANGSU INST OF NUCLEAR MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The current ELISA and CLA are easy to operate and can quantitatively detect MP-IgG, but it takes a long time, the entire reaction time takes more than 1 hour, and ELISA cannot meet the needs of on-the-spot detection, so if the reagent sensitivity and quantitative technical requirements can be guaranteed At the same time, if a rapid, single-serve detection reagent can be provided, it will help clinicians to diagnose quickly and shorten the waiting time of patients

Method used

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  • Immunodetection kit for mycoplasma pneumoniae antibody IgG, and preparation method and use method thereof
  • Immunodetection kit for mycoplasma pneumoniae antibody IgG, and preparation method and use method thereof
  • Immunodetection kit for mycoplasma pneumoniae antibody IgG, and preparation method and use method thereof

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Embodiment 1

[0036] The test strip for detecting MP-IgG includes a bottom plate, and absorbent paper, NC film and sample pad that are sequentially pasted on the bottom plate along the length direction of the bottom plate; wherein, the NC film e is pasted on the bottom plate f The middle part; between the absorbent paper d, the NC film e and the sample pad a, in turn and only in contact with the adjacent part and partially overlap; the NC film is provided with T lines b sprayed with MP antigen at intervals , and the C line c sprayed with the secondary antibody; wherein, the T line b is set close to the sample pad a, and the C line c is set close to the absorbent paper d.

[0037] The polymer nanospheres contain europium ions, and the setting of the polymer nanospheres containing europium ions reduces the quenching of rare earth ions in the sample solution and improves the fluorescence intensity emitted by rare earth ions, thus not only improving the detection accuracy, but also It is conven...

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Abstract

The invention discloses an immunodetection kit for mycoplasma pneumoniae antibody IgG, which comprises a fluorescence immunochromatography test strip, a microsphere solution and a sample diluent, thefluorescence immunochromatography test strip comprises absorbent paper, a chromatography membrane, a sample pad and a supporting bottom plate, a detection line and a quality control line are arrangedon the chromatography membrane, and the detection line is coated with MP antigen; in the microsphere solution, microspheres of the microsphere solution are microspheres coupled with anti-human IgG antibodies. By adopting the immunochromatography test strip, the detection rate is increased. Quantitative detection is realized by adopting a fluorescent substance. The structure of thetest strip is changed on the basis of an existing test strip, and a combination pad is removed; different detection steps are adopted, so that the intensity and the sensitivity of specific fluorescence signals are remarkably improved, the measurement range is enlarged, the measurement accuracy is improved, and the detection background noise is reduced.

Description

technical field [0001] The invention belongs to the technical field of immune analysis, and in particular relates to an immunodetection kit for IgG of mycoplasma pneumoniae antibody, a preparation method and a use method thereof. Background technique [0002] Mycoplasma pneumoniae (MP), a cell-wallless prokaryotic cell, can bind to the mannosamine pyruvate receptors of host respiratory mucosal epithelial cells and produce toxins, leading to pneumonia. Serological epidemiological studies have shown that in China, 20.7-38.9% of community-acquired pneumonia patients are caused by MP, ranking first among the pathogens of community-acquired pneumonia in adults. In addition to respiratory symptoms caused by MP, extrapulmonary manifestations such as meningitis, myocarditis, hemolytic anemia, and thrombocytopenic purpura may occur in 20-25% of cases. Timely and effective diagnosis can effectively control the exacerbation of MP infection and reduce the mortality rate. Therefore, th...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/58G01N33/569G01N33/558
CPCG01N33/6854G01N33/582G01N33/587G01N33/56933G01N33/558G01N2469/20Y02A50/30
Inventor 张艺胡志刚周彬郭明明杨润琳谢敏浩张珏范俊
Owner JIANGSU INST OF NUCLEAR MEDICINE
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