Method for rapidly detecting urogenital tract mycoplasmas based on RPA

A technology of urogenital tract and detection method, which is applied in the field of rapid detection of urogenital tract Mycoplasma based on RPA, and achieves the effect of instant detection

Inactive Publication Date: 2020-10-13
台州市中心医院
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Problems solved by technology

[0005] The purpose of the present invention is to provide a rapid detection method based on RPA to urogenital mycoplasma, to solve the problem of establishing a fast, sensitive and real-time detection method proposed in the above background technology, so as to facilitate the screening of mycoplasma for women and men of appropriate age The problem

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  • Method for rapidly detecting urogenital tract mycoplasmas based on RPA
  • Method for rapidly detecting urogenital tract mycoplasmas based on RPA
  • Method for rapidly detecting urogenital tract mycoplasmas based on RPA

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Embodiment Construction

[0028] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative work all belong to the protection scope of the present invention.

[0029] The present invention provides a kind of rapid detection method based on RPA to urogenital mycoplasma, establishes a kind of fast, sensitive, real-time detection method, can effectively carry out the screening of mycoplasma to school-age women and male, please refer to figure 1 ,

[0030] This RPA-based rapid detection method for urogenital mycoplasma comprises the following steps:

[0031] S1: Design and screening of primers and probes: Accordin...

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Abstract

The invention discloses a method for rapidly detecting urogenital tract mycoplasmas based on RPA in the technical field of mycoplasma. The method for rapidly detecting urogenital tract mycoplasmas based on RPA comprises the following steps: S1, designing and screening primers and probes: according to a 16SrRNA gene and a gap gene of MH, strictly designing five pairs of primers by using an oligo 5software according to an amplification kit analysis and design manual and performing bLAST verification. The kit is used for screening the optimal RPA primer. According to the present invention, the same MH positive specimen is subjected to RPA amplification with the five pairs of primers respectively, and each 50 [mu]l of a reaction system contains 29.5 [mu]l of a primer-free rehydration buffer solution. The method can detect mycoplasmas rapidly, sensitively and immediately, and can effectively perform mycoplasma screening on women and males of the right age.

Description

technical field [0001] The invention relates to the technical field of mycoplasma, in particular to a rapid detection method for urogenital mycoplasma based on RPA. Background technique [0002] Mycoplasma belongs to the sunella class, can replicate itself, is the smallest prokaryotic organism without cell walls, and has extremely high requirements for cultivation. z10 including Ureaplasma urealyticum (UU) and Mycoplasma hominis (MH), which are common commensal microorganisms and are opportunistic pathogens that often cause infections of the urogenital tract and external genital tract. z11 Studies have shown that both Ureaplasma urealyticum and Mycoplasma hominis have a high prevalence rate in China in recent years, and the total positive rate of Mycoplasma in females is as high as 54.5%. z20 Among them, Mycoplasma hominis can colonize the human oral cavity, respiratory tract and genitourinary tract. In addition to causing genital infections (urethritis, vaginitis, cervicit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/6844C12Q1/6804C12Q1/04C12R1/35
CPCC12Q1/689C12Q1/6844C12Q1/6804C12Q2521/507C12Q2522/101C12Q2565/625C12Q2563/131
Inventor 王毅超李招云陈晓英杨合慧朱韬谢姣贵
Owner 台州市中心医院
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