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Household intelligent detection reagent kit

A technology of intelligent detection and kits, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of pathogens that are prone to drug resistance and long-term cure, and achieve the effects of low cost, no interference from human factors, and intuitive results

Active Publication Date: 2016-06-15
西安昱子生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Infections caused by different pathogens require symptomatic drug treatment to effectively kill pathogenic bacteria and protect the physiological environment of the reproductive system. Long-term drug abuse will make pathogens prone to drug resistance, resulting in long-term or even more serious consequences

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0030] 1. Preparation of monoclonal antibody against Mycoplasma ascites

[0031] Strains: Mycoplasma hominis and Mycoplasma urolyticum were provided by ATCC Company of the United States. The above strains were used as immunogens to produce monoclonal antibodies.

[0032] Cell line: The sp20 mouse myeloma cell line was provided by the Cell Engineering Research Center of Fourth Military Medical University for cell fusion.

[0033] method:

[0034] Preparation and inactivation of the immunogenic Mycoplasma strains: Add 1ml of the liquid culture medium of Mycoplasma hominis and Mycoplasma urolyticum to the freeze-dried powder of standard strains of Mycoplasma hominis and Mycoplasma urealyticum respectively, after dissolving, pipette the Mycoplasma bacteria respectively The solution was added to 10 bottles of Mycoplasma pneumoniae liquid medium, 10 bottles of Mycoplasma hominis liquid medium, and 10 bottles of Mycoplasma urealyticum liquid medium, and 100 μl was added to each bott...

Embodiment 2

[0145] 1. Preparation of monoclonal antibody against Mycoplasma ascites

[0146] Strains: Mycoplasma hominis and Mycoplasma urolyticum were provided by ATCC Company of the United States. The above strains were used as immunogens to produce monoclonal antibodies.

[0147] Cell line: The sp20 mouse myeloma cell line was provided by the Cell Engineering Research Center of Fourth Military Medical University for cell fusion.

[0148] method:

[0149] Preparation and inactivation of the immunogenic Mycoplasma strains: Add 1ml of the liquid culture medium of Mycoplasma hominis and Mycoplasma urolyticum to the freeze-dried powder of standard strains of Mycoplasma hominis and Mycoplasma urealyticum respectively, after dissolving, pipette the Mycoplasma bacteria respectively The solution was added to 10 bottles of Mycoplasma pneumoniae liquid medium, 10 bottles of Mycoplasma hominis liquid medium, and 10 bottles of Mycoplasma urealyticum liquid medium, and 100 μl was added to each bott...

Embodiment 3

[0260] The household intelligent detection kit is composed of two parts: an electronic reading analysis part and a monoclonal antibody detection reagent. The electronic reading analysis part includes a single-chip microcomputer system, a photoelectric detection system, a battery, a switch K, a liquid crystal display, a probe and Test paper, the composite gold standard detection probe, battery, switch K, and single-chip microcomputer system are sealed in the casing, and the liquid crystal display is installed at the window of the casing; the monoclonal antibody detection reagent includes the monoclonal Antibody, Neisseria gonorrhoeae monoclonal antibody, Candida albicans monoclonal antibody, goat anti-mouse IgG antibody, composite gold-labeled detection probe;

[0261] The test paper comprises a gold standard pad, a nitrocellulose membrane, a sample pad, and an absorbent pad;

[0262] The composite gold-labeled detection probe is coated on the gold-labeled pad, and the composit...

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Abstract

The invention discloses a household intelligent detection reagent kit.The household intelligent detection reagent kit is composed of an electronic reading and analyzing part and a monoclonal antibody detection reagent.The electronic reading and analyzing part comprises a single-chip microcomputer system, a photoelectrical detection system, a battery, a switch K, a liquid crystal displayer, a combined gold-labeled detection probe and test paper.The combined gold-labeled detection probe, the battery, the switch K and the single-chip microcomputer system are sealed in a case, and the liquid crystal displayer is installed at a case window.The monoclonal antibody detection reagent comprises a monoclonal antibody for specific recognition of mycoplasma genitalium, a neisseria gonorrhoeae monoclonal antibody, a candida albicans monoclonal antibody, a goat-anti-mouse IgG antibody and the combined gold-labeled detection probe.The household intelligent detection reagent kit is used for directly detecting infective pathogens based on the monoclonal antibody technology, has the advantages of being high in specificity, free of blood sampling, capable of making an early diagnosis, high in speed and the like, has the automatic detection function, avoids interference of human factors, displays a detection result visually, and is easy and convenient to operate and low in cost.

Description

technical field [0001] The invention relates to a detection technology for gynecological reproductive inflammation, in particular to a miniaturized automatic analysis and diagnosis device for common gynecological infection sources that can be used at home. This kit can differentially diagnose three common pathogens of reproductive tract infection: Mycoplasma, Neisseria gonorrhoeae, and Candida albicans. Background technique [0002] Reproductive inflammation is the most common inflammation of female reproductive organs, and it is a common disease in gynecological clinics. All ages can be affected. [0003] Mainly caused by mycoplasma, bacteria, mold and so on. Normal healthy women, due to anatomical and biochemical characteristics, the vagina has a natural defense function against the invasion of pathogens; when the natural defense function of the vagina is destroyed, pathogens are easy to invade, leading to inflammation, which will further cause physical harm if not treat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
CPCG01N33/56911G01N33/56933
Inventor 高琪黄威权赵锋
Owner 西安昱子生物科技有限公司
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