Urogenital tract infection pathogen multi-detection primer group and detection device comprising same

Abstract

The invention relates to a urogenital tract infection pathogen multi-detection primer group and a detection device comprising the same. The primer group comprises a bottom piece and a cover piece, a first reagent storage groove, a second reagent storage groove and a connection passage are arranged on the bottom piece, the tail end of the second reagent storage groove is connected with an equalization passage, an exhaust system is arranged on the inner side of the equalization passage, and the first reagent storage groove, the connection passage, the second reagent storage groove and the equalization passage are connected with the exhaust system; equalization holes are formed along the equalization passage, and a sealing hole, a second buffer hole and a detection hole which are connected are formed in the radial direction of the equalization holes. One or multiple of Chlamydia trachomatis primer group, a gonococcus primer group, a ureaplasma urealyticum primer group, mycoplasma hominisprimer group and mycoplasma genitalium primer group and an internal reference primer group are contained in the detection holes respectively; an amplification reagent is contained in the second reagent storage groove or the detection holes. By the urogenital tract infection pathogen multi-detection primer group and the detection device, multi urogenital tract infection pathogen detection can be performed, single-sample multi-index simultaneous detection can be realized, and detection flux is increased.

Description

technical field [0001] The invention relates to a multiple detection primer set for urogenital tract infection pathogens and a detection device containing the primer set, belonging to the field of biotechnology. Background technique [0002] Current research has found that Neisseria lindens, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, and Mycoplasma genitalium are pathogens commonly found in urogenital tract infections, which are associated with various urogenital tract inflammations and can lead to infertility Infertility affects the health of mother and child. These pathogens often present as recessive infection, and some studies have reported the existence of mixed infection. This brings great trouble to clinical diagnosis. The current laboratory diagnostic methods mainly include microscopic observation of pathogens, culture inspection, serum immunological detection and genetic diagnosis. Among them, microscopic examination and culture methods hav...

AI Technical Summary

Problems solved by technology

This brings great trouble to the clinical diagnosis

The current laboratory diagnostic methods mainly include microscopic observation of pathogens, culture inspection, serum immunological detection and genetic diagnosis. Among them, microscopic examination and culture methods have the best specificity, but poor sensitivity. High requirements on experimental conditions; long detection period, some pathogens are extremely difficult to cultivate and cannot be quality controlled; the detection rate of patients with mixed infections is low; the sensitivity of immunological detection is low, and pathogen infection has a window period, which i

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