Preparation method for multiple mycoplasma monoclonal antibodies and multi-linked immunoassay reagent

Abstract

The invention relates to a preparation method for multiple mycoplasma monoclonal antibodies and a multi-linked immunoassay reagent. The preparation method is characterized by separately immunizing mice with mycoplasma pneumoniae, mycoplasma hominis and ureaplasma urealyticum for cell fusion and cloning to obtain three monoclonal antibodies with high specificity and high potency. A mycoplasma multi-linked immunoassay reagent is prepared by mixing the three monoclonal antibodies according to a certain ratio, is used for detecting the diseases infected by the three mycoplasmas, and can quickly and accurately detect mycoplasma diseases; and sampling without phlebotomizing does not bring pain to patients, thus providing patients with timely and effective treatment. The multi-linked immunoassay reagent does not generate harmful waste gas or waste water during preparation and use processes, and also does not bring any injury to patients and operators, thereby being an environment-friendly, safe, accurate and sensitive biological preparation.

Description

technical field [0001] The invention relates to preparation methods and applications of various monoclonal antibodies. Background technique [0002] Mycoplasma pneumoniae is an important pathogen of respiratory tract and lung infection in humans, especially children, accounting for 30% to 40% of the population with respiratory tract and lung infection. Ureaplasma urealyticum (Ureaplasma urealyticum) and Mycoplasma hominis are important pathogens of urogenital tract infection, accounting for 35% to 45% of the population with urogenital tract infection. Mycoplasma is the smallest prokaryotic microorganism without a cell wall. Because mycoplasma has no cell wall, there are obvious differences between the treatment of mycoplasma infection and the treatment of bacterial and viral infections. Only early and accurate differential diagnosis can timely and effective treatment of the disease be carried out. [0003] At present, the detection methods for mycoplasma infection in my co...

AI Technical Summary

Problems solved by technology

The disadvantage is that it generally takes 24 hours to produce results, which brings inconvenience to doctors and patients for timely treatment.

[0004] The immunoassay is a method of using the monoclonal antibody of mycoplasma to find the antigen in the secretion of the infection site. This method is simple and quick to operate, the result is accurate, and the whole process does not require expensive equipment. New search, so far, there are no commercial and patent reports of mycoplasma immunoassay reagents prepared with mycoplasma monoclonal antibodies in my country