Kit and method for synchronously detecting multiple genital tract pathogens

Abstract

The invention discloses a kit and method for synchronously detecting multiple genital tract pathogens. The kit comprises a nucleic acid reaction solution; the nucleic acid reaction solution comprises a primer and a probe of gonococcus, a primer and a probe of ureaplasma urealyticum, a primer and a probe of chlamydia trachomatis, a primer and a probe of mycoplasma hominis, a primer and a probe of mycoplasma genitalium and a primer and a probe of herpes simplex virus type 2. Forward primers of the pathogens respectively comprise nucleotide sequences shown as SEQ ID NO.1, SEQ ID NO.4, SEQ ID NO.7, SEQ ID NO.10, SEQ ID NO.13 and SEQ ID NO.16, reverse primers of the pathogens respectively comprise nucleotide sequences shown as SEQ ID NO.2, SEQ ID NO.5, SEQ ID NO.8, SEQ ID NO.11, SEQ ID NO.14 and SEQ ID NO.17, and the probes of the pathogens respectively comprise nucleotide sequences shown as SEQ ID NO.3, SEQ ID NO.6, SEQ ID NO.9, SEQ ID NO.12, SEQ ID NO.15 and SEQ ID NO.18. The kit can be used for rapidly, accurately and synchronously detecting gonococcus, ureaplasma urealyticum, chlamydia trachomatis, mycoplasma hominis, mycoplasma genitalium and herpes simplex virus type 2, and is relatively strong in specificity and relatively high in sensitivity.

Description

technical field [0001] The invention belongs to the field of biological kits, and relates to a kit and method for synchronous detection of multiple reproductive tract pathogens, in particular to a kit and method for synchronous detection of six reproductive tract pathogens based on a PCR fluorescent probe method. Background technique [0002] STDs are one of the most serious and widespread infectious diseases that endanger human beings. It not only endangers personal health, but also affects families and future generations. Sexually transmitted diseases can damage human health in many ways. If sexually transmitted diseases cannot be detected and treated thoroughly after infection, they will not only damage human reproductive organs and cause infertility, but some diseases can also damage important organs of the human body such as the heart and brain, and even cause infertility. die. Some diseases are difficult to cure once infected, such as genital warts and genital herpes....

AI Technical Summary

Problems solved by technology

[0008] At present, although there are patent documents that use real-time fluorescent quantitative PCR to quickly detect pathogens related to various reproductive tract infections at the same time, the sensitivity is low, and there is no report that can target the above-mentioned six specific pathogens (ie, Neisseria gonorrhoeae, urea trachomatis, mycoplasma hominis, mycoplasma genitalium, herpes simplex virus type 2) for simultaneous detection

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