Detection system capable of curing sexually transmitted infection pathogens and a kit and application thereof

Abstract

The invention relates to a detection system capable of curing sexually transmitted infection pathogens and a kit and application thereof. The detection system comprises six pairs of primers for detecting treponema pallidum, trichomonad vaginalis, chlamydia trachomatis, neisseria gonorrhoeae, ureaplasma urealyticum and mycoplasma hominis, one pair of human DNA internal reference primers and one pair of system quality control internal reference primers. According to the detection system capable of curing the sexually transmitted infection pathogens and the kit thereof, the steps of conventional culture and the like are not needed, synchronous identification and semi-quantitative analysis of six pathogens difficult to culture can be directly carried out on a genital tract secretion sample in the same reaction system, and the detection system and the kit have the advantages of high specificity, high sensitivity, good repeatability, rapidness and accuracy; a comprehensive, accurate and low-cost etiological diagnosis basis can be provided for clinic and patients in the first time, and an important reference is provided for individualized medication and accurate medical treatment.

Description

technical field [0001] The invention relates to a multiple gene detection product and a detection system used in the product, belonging to the field of biotechnology. Background technique [0002] Sexually transmitted infections (STIs) are one of the more common infectious diseases clinically. They refer to diseases transmitted through sexual contact. The main lesion is in the reproductive system. It can also cause infertility, and even involve the nervous, cardiovascular, skeletal and other systems, causing local or systemic lesions. [0003] Curable pathogens that cause STIs mainly include Treponema pallidum, Neisseria gonorrhoeae, Mycoplasma, Chlamydia, Trichomonas vaginalis, etc. Among them, Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Trichomonas vaginalis (TV) and Treponema pallidum (TP) are the four most common pathogens of curable sexually transmitted infections, and since 1995, the World Health Organization (WHO) conducts regular assessments of the glob...

AI Technical Summary

Problems solved by technology

[0006] In summary, the current detection and diagnosis methods for common curable sexually transmitted infection difficult-to-culture pathogens cannot meet clinical needs, and a detection technology and method that can simultaneously and quantitatively detect the above-mentioned several common sexually transmitted infection difficult-to-culture pathogens is urgently needed. So as to help patients and clinicians timely and comprehensively screen high-risk sexually transmitted pathogens, discover potential infections, and take timely treatment

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